TITILE OF PROJECT : THE RESEARCH OF THE DEVELOPING OF SYSTEM OF SUPERE ULTRASHORTWAVE IRRADIATION AND MEASUREMENT OF TEMPERATURE USING DIFFERNTIAL HYPOTHERMIA TREATMENT OF MALIGNANT BRAIN TUMOR

恶性脑肿瘤超短波照射及差温低温测温系统的研制利用2451 MHz微波辐射系统，通过设定目标加热温度，自动控制输出装置和非接触式施加器，研究了加热活性。1)体模：我们进行了照射，涂抹器距离它7厘米，可以加热它的直径20厘米和深度20毫米。2)对10只正常猴进行差式低温治疗。我们可以从大脑表面加热直径4厘米，深度10毫米的白质，温度为37ﾟC.2。采用吸氢清除法测定脑白质距脑表面10 mm处局部脑血流量，为差别低温治疗提供生理学依据。我们的结果是，当加热到37ﾟC时，局部脑血流量比…前增加了约2个小时更多的重新加热。加热后局部脑血流量恢复到加热前的水平。我们对10例病毒诱发的猴脑肿瘤进行了差热治疗。治疗后即刻，患侧以脑表面为中心出现脑水肿，24小时后水肿消退。CT随访5例，3例肿瘤体积缩小，1例肿瘤体积增大。观察差热治疗对大鼠脑组织及血液中ACNU强度的影响。方法：1)对照组，在全身麻醉和常温条件下静脉注射阿昔洛韦(20 mg/只)，2)常规低温治疗，1周后行双侧开颅，用全自动控温微波照射系统和非接触式敷贴器加热同侧猴一侧脑，恒温(37~40ﾟ℃)，给药后立即抽血及脑组织(给药侧、非给药侧)测量浓度：即刻冰冻，用高效液相色谱法和药动学分析法测定ACNU浓度。结果：1)ACNU在血液中的半衰期比常温下的对照组延长了两个小时。2)ACNU在脑组织中的浓度绝对值在给药侧与非给药侧无差异，但一室开放模型分析显示给药侧ACNU的半衰期延长。结论：经差热降温治疗后，ACNU在血液中的浓度与常温相比维持在较高水平，减少了内脏器官的损伤。此外，由于它在床面以接近常温的速度代谢和分解，我们认为两者联合治疗将获得较少的抗肿瘤增强作用。

Developing of system of super ultrashortwave irradiation and measurement of temperature using differential hypothermia treatment of malignant brain tumor1. we studied the activity of heating by the using of the 2451MHz microwave irradiation system with establishment of objective heeting temperature and automatic control of output device and non contact applicator. 1) phantom : we performed iraddiating that applicator was 7cm away from it we can heat it 20cm diameter & 20mm depth. 2) differential hypothermia treatment was performed by 10 normal monkey brians. we can heat white matter 4cm diameter & 10mm depth from the brain surface at the degree of 37ﾟC.2. we measured regional cerebral blood flow of the white matter 10mm depth from the brain surface by the inhalation hydrogen clearance method for physiological basis at the differential hypothermia treatment. we had the result that when heat it at the degree of 37ﾟC regional cerebral blood flow increased about two hold compared with befo … More re heating. And after the heating regional cerebral blood flow came back to before heating.3. we performed differential hypothermia treatment to 10 virally induced monkey brain tumors. The brain of performed side was revealed edema at centering around the brain surface immediately the treatment, but after 24hours edema was extinguished. Follow up-CT was performed to the 5,3 were revealed the decreasing the tumor size, but 1 was revealed the increasing the tumor size.4. We examined the effect of differential hypothermia treatment influence of strength of ACNU in the blood & tissue of brain. method : 1) control ; ACNU (20mg/body) was prescrived (iv) in the general anethsezia and at norotemperature, and drew the blood 2) differntial hypothermia treatment ; After 1 week ago, we performed bilateral craniectomy and heating to the one side brain of the same monkey by the 245GHz microwave irradiation system with automatic control of establishment temperature and applicator of non touch, and keep the temperature (37-40ﾟC) and after prescriving ACNU drew the blood and brain tissue (heeting side, non heeting side) 3) measurement concentration of ACNU : we freezed it immediatry, and measured concetration of ACNU by HPLC and analised pharmacokinetics. result : 1) a half period of ACNU (in the blood) in the differential hypothermia treatment was extended two hold compared with contorol in the normal temperature. 2) there was no differeciation that absolute value of concentration of ACNU in the tissue of the brain among the heeting side and non heeting side, but extending of a half period of ACNU of the heeting side was revealed by the analysis of one compartment open model. conclusion : by the differential hypothermia treatment concetration of ACNU in the blood was keeped high volume compare with in the normal temperature cause of metabolism and excrtio of the ninternal organs was delaid. further for that was metabolized and taken apart to pieces speed of nearly of normal temperature on the heeting side, we consider that combine therapy of two will acquire the increasing effect of antitumor Less