THE RESEARCH OF THE DIFFERENTIAL HYPOTHERMIA THERAPY FOR BRAIN TUMORS

差异低温治疗脑肿瘤的研究

• 批准号: 56480242
• 负责人: NISHIMOTO Akira
• 金额: $ 2.18万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1981
• 资助国家: 日本
• 起止时间: 1981 至 1983
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-56480242/
• 关键词: DIFFERNTIAL HYPOTHERMIA; BRAIN TUMOR CELLS; ACNU

Effect of Differential Hypothemia on Cultured Brain Tumor Cells1. The effects of combined use of heat with drug were studied by colony forming assay, using a cultured rat glioma cell line (C6). The cells were treated with a nitrosourea derivative ACNU in different thermal conditions.After treament of 43ﾟC for 1 or 2 hours, the surviving fraction reduced to 76% or 28% of non-treatment cells. While with 5mug/ml ACNU,the surviving fraction reduced to 55% or 19% and with 20mug/ml ACNU, 3% or 0.5%, respectively. These results proved that hyperthermialead to marked increase of cytocidal effect of ACNU and suggested the synergestic activity of ACNU and differential hypothermia to achieve greater tumoricidal effect.2. An experimental brain tumor model was established with VX2 tumor cells stereotactically transplanted in the rabbit brain. On the other hand, to investigate the thermal distribution in the normal brain under 915MHz microwave irradiation, the thermal distribution under 915MHz and 2.450MHz microwave irradiation were compared. More uniform and relatively deeper heeting was achieved with 915MHz microwave irradiation than with 2.450MHz. However, it seemed quite difficult for the generator presently used for the 915MHz irradiation, whose maximal generating power is 45W,to keep the brain over 37ﾟC under the systemic hypothermia of 30ﾟC.Therefore, reinforcement of the generator seemed to be necessary. Furthrmore, further heating effect could achieved to fill aluminium oxide between the microwave antenna and the object than air gap. No macro-or microscopically pathologic change was seen after 5-hour continuous heating of the brain under systemic hypothermia of 30ﾟC.3. To evaluate the tumoricidal effect of differential hypothermia, a Rous Sarcoma virus-induced brain tumor model was established in adult monkeys

低低温对培养脑肿瘤细胞的影响[j]。以培养的大鼠胶质瘤细胞系（C6）为实验对象，采用菌落形成实验研究了热药联合使用的作用。用亚硝基脲衍生物ACNU在不同的热条件下处理细胞。处理1或2小时后，存活率下降到76%或28%的非处理细胞。5杯/毫升ACNU时，存活率分别降至55%或19%，20杯/毫升ACNU时，存活率分别降至3%或0.5%。这些结果证明，高温导致ACNU的杀细胞作用明显增强，提示ACNU与差异低温的协同作用可达到更大的杀肿瘤效果。用VX2肿瘤细胞立体定向移植兔脑建立实验性脑肿瘤模型。另一方面，为了研究915MHz微波辐射下正常脑组织的热分布，比较915MHz和2.450MHz微波辐射下的热分布。与2.450MHz相比，915MHz的微波辐射获得了更均匀、更深的覆盖。然而，目前用于915MHz辐射的发电机，其最大发电功率为45W，在30 <s:1>的全身低温下，很难使大脑保持在37 C以上。因此，加强发电机似乎是必要的。此外，在微波天线与物体之间填充氧化铝可以获得比气隙更大的加热效果。在30℃(3)的全身低温下连续加热5小时后，未见宏观或微观病理改变。为了评估差异低温的杀瘤效果，我们在成年猴子身上建立了劳斯肉瘤病毒诱导的脑肿瘤模型

项目成果

A.NISHIMOTO, T.SATOH, S.NAKASONE, et al: "TREATMENT OF MALIGNANT BRAIN TUMORS BY DIFFERENTIAL HYPOTHERMIA." 8TH INTERNATIONAL CONGRESS OF NEUROLOGICAL SURGERY (TRONT) JULY. (1985)

A.NISHIMOTO、T.SATOH、S.NAKASONE 等人：“通过差别低温治疗恶性脑肿瘤。”

K. Tabuchi, A. Nishimoto, K. Matsumoto, T. Satoh, S. Nakasone, T. Fujiwara, H. Ogura: "Establishment of a brain tumor model in adult monkeys" J. Meurosurg.63. 912-916 (1985)

K. Tabuchi、A. Nishimoto、K. Matsumoto、T. Satoh、S. Nakasone、T. Fujiwara、H. Ogura：“成年猴子脑肿瘤模型的建立”J. Meurosurg.63。

仲宗根進: "培養グリオーマ細胞に対する温熱の殺細胞効果に関する研究-特にACNUとの併用効果について-" 岡山医学会雑誌. 99. 1151-1166 (1987)

Susumu Nakasone：“热疗对培养神经胶质瘤细胞的杀细胞作用的研究 - 特别是与 ACNU 联合使用的作用 -”冈山医学会杂志 99. 1151-1166 (1987)。

松本健五,仲宗根進,原田泰弘,田淵和雄,西本詮,: "915MHzマイクロウェーブによる脳加温効果の検討." 癌の臨床. 28. 835-840 (1982)

Kengo Matsumoto、Susumu Nakasone、Yasuhiro Harada、Kazuo Tabuchi、Akira Nishimoto，：“915MHz 微波的大脑变暖效应检查”28. 835-840 (1982)。

K.TABUCHI,AKIRA NISHIMOTO,K MATSUMOTO et al: "ESTABLISHMENT OF A BRAIN-TUMOR MODEL IN ADULT MONKEY" J NEUROSURG. 63. 912-916 (1985)

K.TABUCHI、AKIRA NISHIMOTO、K MATSUMOTO 等人：“成年猴脑肿瘤模型的建立”J NEUROSURG。