Development of antibacterial drugs based on insect antibacteria proteins

Sarcotoxin I是一种有效的抗菌蛋白，当用皮下针头刺穿peregrina Sarcophaga幼虫的体壁时，在其血淋巴中产生。我们将该蛋白纯化至均匀性，发现它由三种蛋白组成，称为肌毒素IA， IB和IC，它们具有几乎相同的初级结构。将各组分纯化至均匀性，并测定其氨基酸序列。结果表明，每一种蛋白质由39个氨基酸残基组成，3种蛋白质之间的差异仅为2 - 3个氨基酸残基。肌毒素I的原发部位为细菌膜。在大肠杆菌中加入肌毒素I后，大肠杆菌的膜电位几乎瞬间消失，导致主动转运和ATP生成的丧失。已分离到一个肌毒素IA的cDNA克隆。综上所述，我们得出结论，肌毒素I是一种非常有用的物质，它的应用范围很广，包括抗菌药物。

Sarcotoxin I is a potent antibacterial protein induced in the hemolymph of Sarcophaga peregrina larvae when their body wall was pricked with a hypodermic needle. We purified this protein to homogeneity and found that it consists of three proteins termed sarcotoxin IA, IB and IC having almost identical primary structure. Each component was purified to homogeneity and its amino acid sequence was determined. It was found that each protein consists of 39 amino acid residues and difference among three proteins is in only 2 - 3 amino acid residues.Primary site of sarcotoxin I was shown to be bacterial membrane. On addition of sarcotoxin I to E. coli, mimbrane potential of E. coli disappeared almost instantaneously, resulting in the loss of active transport and ATP generation. A cDNA clone for sarcotoxin IA has been isolated.In summary, we concluded that sarcotoxin I is a very useful substance and there are many applications of this substance including antibacterial drugs.