Analysis of Sarcophaga lectin receptor on the surface of murine macrophages

小鼠巨噬细胞表面Sarcophaga凝集素受体的分析

• 批准号: 61480458
• 负责人: NATORI Shunji
• 金额: $ 3.84万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-61480458/
• 关键词: Sarcophaga lectin; macrophages; receptor; membrane protein; Ehrlich ascites tumor cells; J774.1 cells; モノクローナル抗体; センチニクバエレクチン; J774.1

The structure of Sarcophaga lectin receptor on the surface of murine macrophages was analyzed using monoclonal antibodies. This receptor was found by gel filteration to have a molecular weight of 460 kDa. SDS-polyacrylamide gel electrophoresis showed that this receptor consists of two subunits of 170 kDa and 110 kDa Results indicated that it is probably a heterotetramer of two molecules of each subunit.It was shown that the level of cyclic AMP decreases significantly, on treatment of murine macrophages with this lectin. Therefore, it was suggested that G-protein is involved in the process of the activation of macrophages by Sarcophaga lectin.

用单克隆抗体分析了鼠巨噬细胞表面的麻蝇凝集素受体的结构。通过凝胶过滤发现该受体具有460 kDa的分子量。SDS-聚丙烯酰胺凝胶电泳结果表明，该受体由170 kDa和110 kDa两个亚基组成。结果表明，该受体可能是由每个亚基的两个分子组成的异源四聚体。提示G蛋白参与了麻蝇凝集素激活巨噬细胞的过程。

项目成果

名取俊二: 免疫薬理. 4. 377-381 (1986)

名取俊二：免疫药理学。4. 377-381 (1986)

Shunji,Natori: Alan R. Liss, Inc., New York. Molecular Entomology, 369-378 (1987)

名取俊二：Alan R. Liss, Inc.，纽约。

Kohji Ueno: Journal of Biological Chemistry. 262. 12780-12784 (1987)

Kohji Ueno：生物化学杂志。

Yoshiaki Ohkuma: Cancer Resarch. 46. 3648-3652 (1986)

大隈义明：癌症研究。

名取俊二: 生化学. 58. 1300-1304 (1986)

名取俊二：生物化学 58。1300-1304 (1986)