Analysis of Sarcophaga lectin receptor on the surface of murine macrophages

用单抗分析了小鼠巨噬细胞表面Sarcophaga凝集素受体的结构。凝胶过滤发现该受体的相对分子质量为460 kDa。SDS-聚丙烯酰胺凝胶电泳法显示该受体由170 kDa和110 kDa两个亚基组成。结果表明，该凝集素可能是由每个亚基上的两个分子组成的异构体。因此，G蛋白参与了肉噬菌凝集素激活巨噬细胞的过程。

The structure of Sarcophaga lectin receptor on the surface of murine macrophages was analyzed using monoclonal antibodies. This receptor was found by gel filteration to have a molecular weight of 460 kDa. SDS-polyacrylamide gel electrophoresis showed that this receptor consists of two subunits of 170 kDa and 110 kDa Results indicated that it is probably a heterotetramer of two molecules of each subunit.It was shown that the level of cyclic AMP decreases significantly, on treatment of murine macrophages with this lectin. Therefore, it was suggested that G-protein is involved in the process of the activation of macrophages by Sarcophaga lectin.