Identification of the active domain of insect antibacterial proteins and production of new antibacterial peptides.

昆虫抗菌蛋白活性结构域的鉴定及新型抗菌肽的生产。

• 批准号: 07557152
• 负责人: NATORI Shunji
• 金额: $ 6.02万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557152/
• 关键词: antibacterial peptide; insect; MRSA; defense; active domain; alpha-helix; α-ヘリックス; 活性ドメイン

An antibacterial peptide, KLKLLLLLKLK-NH_2 (L5) showed significant chemotherapeutic activity in MRSA-infected mice in vivo. The antibacterial mechanisms of L5 were investigated. L5 was shown to have the activity to release the proton gradient using the bacterial membrane. The strength of the activity of L5 was between CCCP and DNP.And L5 was also shown to inhibit the activity of signal peptidase. Thus these effects of L5 seem to be the main reason of the antibacterial activity. Recently we found that L5 activated the human neutrophil, resulting the production of superoxide. Neither KLKLLLLKLK-NH_2 (L4), nor KLKLLLKLK-NH_2 (L3) has the activity at all, indicating that the number of Leu is important for activating neutrophil.

抗菌肽KLKLLLLLKLK-NH_2（L5）对MRSA感染的小鼠具有明显的体内化疗活性。对L5的抗菌机理进行了初步探讨。L5被证明具有使用细菌膜释放质子梯度的活性。L5的活性强度介于CCCP和DNP之间，对信号肽酶也有抑制作用。因此，L5的这些作用似乎是抗菌活性的主要原因。最近，我们发现L5激活人中性粒细胞，导致超氧化物的产生。KLKLLLLKLK-NH_2（L4）和KLKLLLKLK-NH_2（L3）均无此活性，提示Leu的数量对中性粒细胞的活化起重要作用。

项目成果

Yuki Nakajima: "A Novel Protease in the Pupal Yellow Body of Sarcophaga peregrina(Flesh Fly)" J.Biol.Chem.272(38). 23805-23810 (1997)

Yuki Nakajima：“Sarcophaga peregrina（肉蝇）蛹黄体中的一种新型蛋白酶”J.Biol.Chem.272(38)。

Juan Alvarez-Bravo, Shoichiro Kurata and Shunji Natori: "Mode of action of an antibacterial peptide, KLKLLLLLKLK-NH_2" J.Biochem.117. 1312-1316 (1995)

Juan Alvarez-Bravo、Shoichiro Kurata 和 Shunji Natori：“抗菌肽 KLKLLLLLKLK-NH_2 的作用模式”J.Biochem.117。

Yutaka Hirakura: "Selective interaction of synthetic antimicrobial peptides derives from sapecin B with lipid bilayers" J.Biochem.120. 1130-1140 (1996)

Yutaka Hirakura：“源自 sapecin B 的合成抗菌肽与脂质双层的选择性相互作用”J.Biochem.120。

Takahiro Ito, Qihong Xu, Hideaki Takeuchi, Takeo Kubo and Shunji Natori: "Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II" FEBS Lett.385. 21-24 (1996)

Takahiro Ito、Qihong Xu、Hideaki Takeuchi、Takeo Kubo 和 Shunji Natori：“小鼠睾丸特异性转录延伸因子 S-II 基因的精母细胞特异性表达”FEBS Lett.385。

Shunji Natori and Takeo Kubo: "Role of lectins in development and morphogen in insects" New Direction in Invertebrate Immunology. (1997)

Shunji Natori 和 Takeo Kubo：“凝集素在昆虫发育和形态发生素中的作用”无脊椎动物免疫学新方向。