Analysis of the receptor of an antibacterial peptide on human neutrophils.

人中性粒细胞上抗菌肽受体的分析。

• 批准号: 08458192
• 负责人: NATORI Shunji
• 金额: $ 4.74万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458192/
• 关键词: antibacterial peptide; insect; neutrophil; superoxide; receptor; pertussis toxin; GTP-binding protein; バクテリア; サ-ベシンB

An antibacterial peptide, KLKLLLLLKLK-NH_2 (L5) showed significant chemotherapeutic activity in MRSA-infected mice in vivo. We found that L5 activated the human neutrophil, resulting the production of superoxide. The binding protein with a molecular mass of 55 kDa was purified from membrane proteins of human neutrophil using affinity column chromatography conjugated with L5. The pertussis toxzin inhibited the activation of L5 to the neutrophil, suggesting that the signal of L5 into the neutrophil is mediated via GTP-binding protein. The binding protein with a molecular mass of 55 kDa is expected to be the target molecule to search for effective drugs for some infectious diseases.

抗菌肽 KLKLLLLLKLK-NH_2 (L5) 在 MRSA 感染的小鼠体内表现出显着的化疗活性。我们发现 L5 激活人类中性粒细胞，导致超氧化物的产生。使用与L5缀合的亲和柱层析从人中性粒细胞的膜蛋白中纯化出分子量为55 kDa的结合蛋白。百日咳毒素抑制 L5 对中性粒细胞的激活，表明 L5 进入中性粒细胞的信号是通过 GTP 结合蛋白介导的。分子量为55 kDa的结合蛋白有望成为寻找治疗某些传染病有效药物的靶分子。

项目成果

Yuki, Nakajima: "A Novel Protease in the Pupal Yellow Body of Sarcophaga pergrina(Flesh Fly)" J.Biol.Chem.272(38). 23805-23810 (1997)

Yuki, Nakajima：“Sarcophaga pergrina（肉蝇）蛹黄体中的一种新型蛋白酶”J.Biol.Chem.272(38)。

Kiyoshi Kawasaki: "Presence of the Periplaneta Lectin-related Protein Family in the American Cockroach Periplaneta americana" Insect Biochem.Molec.Biol.26. 355-364 (1996)

Kiyoshi Kawasaki：“美洲大蠊凝集素相关蛋白家族在美洲大蠊中的存在”Insect Biochem.Molec.Biol.26。

Yuki Nakajima, Juan Alvarez-Bravo, Jang-Hyun Cho, Ko-ichi Homma, Shiro Kanegasaki and Shunji Natori: "Chemotherapeutic activity of synthetic antimicrobial peptides : Correlation between chemotherapeutic acitvity and neutrophil-activating activity" FEBS Le

Yuki Nakajima、Juan Alvarez-Bravo、Jang-Hyun Cho、Ko-ichi Homma、Shiro Kanegasaki 和 Shunji Natori：“合成抗菌肽的化疗活性：化疗活性与中性粒细胞激活活性之间的相关性” FEBS Le

Takahiro Ito: "Spermatocyte-specific expression of the gene for mouse testis-specific transcription elongation factor S-II." FEBS Letters. 385. 21-24 (1996)

Takahiro Ito：“小鼠睾丸特异性转录延伸因子 S-II 基因的精母细胞特异性表达。”

Yuki Nakajima: "A Novel Protease in the Pupal Yellow Body of Sarcophaga peregrina(Flesh Fly)" J.Biol.Chem.272(38). 23805-23810 (1997)

Yuki Nakajima：“Sarcophaga peregrina（肉蝇）蛹黄体中的一种新型蛋白酶”J.Biol.Chem.272(38)。