Defense mechanism of insect (Sarcophaga peregrina)

昆虫（Sarcophaga peregrina）的防御机制

• 批准号: 04102006
• 负责人: NATORI Shunji
• 金额: $ 112万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Specially Promoted Research
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-04102006/
• 关键词: Sarcophaga; Defense system; Development; Antibacterial protein; Sapecin; Imaginal disc; Ptassium channel; Proteinase; 個体発生; カリウムチンネル; プロテア-ゼ; 転写因子; Sarcophagaレクチン; レクチンリセプター

Insect have developed a unique defense system against various outside stimuli such as microbial infection and integmental injury. We have been studying various defense proteins of Sarcophaga peregrina (the flesh fly). These proteins include a lectin and several antibacterial proteins. Throughout these studies, we came to the conclusion that there are two important novel aspects of these defense proteins. One is that a single defense protein probably plays at least two roles in defense and development. Insects probably have the flexibility to use one defense protein in multiple ways. The other interesting aspect is the phylogenetical significance of the insect defense system. Insect defense system is possibly a prototype of the mammalian defense system. Except these two important aspects, we obtained various important findings on insect immnity. Most of these findings have been published.

昆虫对外界的各种刺激，如微生物感染、表皮损伤等，形成了独特的防御系统。我们一直在研究各种防御蛋白的Sarcophaga peregrina（肉蝇）。这些蛋白质包括凝集素和几种抗菌蛋白。通过这些研究，我们得出结论，这些防御蛋白有两个重要的新方面。一个是单个防御蛋白可能在防御和发育中至少起两种作用。昆虫可能具有以多种方式使用一种防御蛋白的灵活性。另一个有趣的方面是昆虫防御系统的遗传学意义。昆虫防御系统可能是哺乳动物防御系统的原型。除了这两个重要方面，我们还获得了许多关于昆虫免疫力的重要发现。这些研究结果大部分已经发表。

项目成果

T.Nakanishi, A.Nakano, K.Nomura, K.Sekimizu and S.Natori: "Purification, gene cloning, and gene disruption of the transcription elongation factor S-II in Saccharomyces cerevisiae." J.Biol.Chem.267. 13200-13204 (1992)

T.Nakanishi、A.Nakano、K.Nomura、K.Sekimizu 和 S.Natori：“酿酒酵母转录延伸因子 S-II 的纯化、基因克隆和基因破坏。”

N.Takahashi, S.Kurata and S.Natori: "Molecular cloning of cDNA for the 29kDa proteinase participating in decomposition of the larval fat body during metamorphosis of Sarcophaga peregrina (flesh fly)." FEBS Letts.334. 153-157 (1993)

N.Takahashi、S.Kurata 和 S.Natori：“29kDa 蛋白酶的 cDNA 分子克隆，该蛋白酶参与游隼（肉蝇）变态过程中幼虫脂肪体的分解。”

Masafumi Shimoda: "Inhibition of the Ca^<2+>-activated K^+-channel by sapecin B、an insect antibacterial protein" FEBS Letts.339. 59-62 (1994)

Masafumi Shimoda：“昆虫抗菌蛋白 sapecin B 对 Ca^2+-激活的 K^+-通道的抑制”FEBS Letts.339 (1994)。

Katsushige Yamada: "Characterization of the antibacterial peptide derived from sapecin B、anantibacterial protein of Sarcophaga peregrina(flesh fly)" Biochem.J.298. 623-628 (1994)

Katsushige Yamada：“来自 sapecin B 的抗菌肽的表征，sapecin B 是游隼（肉蝇）的抗菌蛋白”Biochem.J.298（1994）。

Chalk R: "Purification of an insect defensin from the mosquito, Ades aeqypti" Insect Biochem. Mol. Biol.24. 403-410 (1994)

Chalk R：“从蚊子 Ades aeqypti 中纯化昆虫防御素”Insect Biochem。