Automated high-performance apparatus for quantitative immunoblot analysis: Manufacture trial and its application to cell biochemistry

1. 免疫印迹定量测定基本条件的建立（1984年）。(1)在过氧化物酶作用下，蛋白质在5 ng ~ 10 <mu> g范围内呈线性关系。(2)高速数据处理使快速扫描（3厘米/秒）和1m字节的内存存储足以进行定量分析。研制了用于细胞内分析的试验机和基础实验（1985年）。浓度与吸光度在光密度0 ~ 2.2范围内呈线性关系，灵敏度最高。完成了一台测试机器和细胞内分析的实际研究（1986年）。对calpain和calpastatin在不同组织中的生化和免疫组织化学分布进行了研究，并通过免疫印迹法估计了这些蛋白的定量变化。

1. Establishment of fundamental conditions for quantitative determination of immunoblots (1984). (1) A linear relationship in the range of 5 ng to 10 <mu> g of proteins was established using peroxidase. (2) High speed data processing enabled rapid scanning (3 cm/sec) and 1 M byte memory storage sufficed quantitative analysis.2. Development of a testing machine and the fundamental experiment for applications to intracellular analysis (1985). Concentration vs. absorbance was linear over the range of optical density of 0 to 2.2 and the best sensitivity was obtained.3. The completion of a testing machine and practical studies on intracellular analysis (1986). Biochemical and immunohistochemical distribution studies in various tissues with regard to calpain and calpastatin were advanced, and quantitative variation of these proteins was estimated by immunoblotting.