Automated high-performance apparatus for quantitative immunoblot analysis: Manufacture trial and its application to cell biochemistry

1.免疫印迹定量测定基本条件的建立（1984）。(1)使用过氧化物酶在5 ng至10 g蛋白质范围内建立线性关系<mu>。(2)高速数据处理使快速扫描（3cm/sec）成为可能，1 M字节的内存足以进行定量分析.开发试验机和应用于细胞内分析的基础实验（1985年）。浓度与吸光度在光密度0 ~ 2.2范围内呈线性关系，灵敏度最高.完成细胞内分析的试验机和实际研究（1986年）。生物化学和免疫组织化学分布在各种组织中的钙蛋白酶和钙蛋白酶抑制剂的研究进展，这些蛋白质的定量变化估计免疫印迹。

1. Establishment of fundamental conditions for quantitative determination of immunoblots (1984). (1) A linear relationship in the range of 5 ng to 10 <mu> g of proteins was established using peroxidase. (2) High speed data processing enabled rapid scanning (3 cm/sec) and 1 M byte memory storage sufficed quantitative analysis.2. Development of a testing machine and the fundamental experiment for applications to intracellular analysis (1985). Concentration vs. absorbance was linear over the range of optical density of 0 to 2.2 and the best sensitivity was obtained.3. The completion of a testing machine and practical studies on intracellular analysis (1986). Biochemical and immunohistochemical distribution studies in various tissues with regard to calpain and calpastatin were advanced, and quantitative variation of these proteins was estimated by immunoblotting.