Automated high-performance apparatus for quantitative immunoblot analysis: Manufacture trial and its application to cell biochemistry

1.建立定量测定免疫印迹的基本条件(1984)。(1)用过氧化物酶建立了蛋白质在5 ng~10 ng范围内的线性关系。(2)高速数据处理实现了快速扫描(3 cm/s)和1M字节的内存存储，足以进行定量分析。用于细胞内分析的测试机和基础实验的发展(1985)。浓度与吸光度在0~2.2范围内呈线性关系，获得了最佳的灵敏度。完成了一台测试机和细胞内分析的实用研究(1986)。关于calain和calastatin在不同组织中的生化和免疫组织化学分布研究进展，并用免疫印迹法估计这些蛋白的数量变化。

1. Establishment of fundamental conditions for quantitative determination of immunoblots (1984). (1) A linear relationship in the range of 5 ng to 10 <mu> g of proteins was established using peroxidase. (2) High speed data processing enabled rapid scanning (3 cm/sec) and 1 M byte memory storage sufficed quantitative analysis.2. Development of a testing machine and the fundamental experiment for applications to intracellular analysis (1985). Concentration vs. absorbance was linear over the range of optical density of 0 to 2.2 and the best sensitivity was obtained.3. The completion of a testing machine and practical studies on intracellular analysis (1986). Biochemical and immunohistochemical distribution studies in various tissues with regard to calpain and calpastatin were advanced, and quantitative variation of these proteins was estimated by immunoblotting.