Automated high-performance apparatus for quantitative immunoblot analysis: Manufacture trial and its application to cell biochemistry

用于定量免疫印迹分析的自动化高性能装置：制造试验及其在细胞生物化学中的应用

• 批准号: 59870011
• 负责人: MURACHI Takashi
• 金额: $ 11.07万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-59870011/
• 关键词: protein; qualitative analysis; microquantitative determination; densitometer; data processing; immunoblotting; multiple staining; 細胞内成分分析; イムノブロット; ウエスタンブロット; 細胞生化学

1. Establishment of fundamental conditions for quantitative determination of immunoblots (1984). (1) A linear relationship in the range of 5 ng to 10 <mu> g of proteins was established using peroxidase. (2) High speed data processing enabled rapid scanning (3 cm/sec) and 1 M byte memory storage sufficed quantitative analysis.2. Development of a testing machine and the fundamental experiment for applications to intracellular analysis (1985). Concentration vs. absorbance was linear over the range of optical density of 0 to 2.2 and the best sensitivity was obtained.3. The completion of a testing machine and practical studies on intracellular analysis (1986). Biochemical and immunohistochemical distribution studies in various tissues with regard to calpain and calpastatin were advanced, and quantitative variation of these proteins was estimated by immunoblotting.

1.建立定量测定免疫印迹的基本条件(1984)。(1)用过氧化物酶建立了蛋白质在5 ng~10 ng范围内的线性关系。(2)高速数据处理实现了快速扫描(3 cm/s)和1M字节的内存存储，足以进行定量分析。用于细胞内分析的测试机和基础实验的发展(1985)。浓度与吸光度在0~2.2范围内呈线性关系，获得了最佳的灵敏度。完成了一台测试机和细胞内分析的实用研究(1986)。关于calain和calastatin在不同组织中的生化和免疫组织化学分布研究进展，并用免疫印迹法估计这些蛋白的数量变化。

项目成果

信原美子: 日本臨床化学会年会記録. 25. 90 (1985)

Yoshiko Nobuhara：日本临床化学学会年会记录 25. 90 (1985)。

村地孝: 病態生理. 6. 133-139 (1987)

村落隆：病理生理学。6。133-139（1987）

MURACHI Takashi: "The Biological Role of Rroteinases and Their Inhibitors in Skin." University of Tokyo Press, 10 (1985)

MURACHI Takashi：“蛋白质酶及其抑制剂在皮肤中的生物学作用。”

高野恵美子: 生化学. 57. 772 (1985)

高野惠美子：生物化学 57. 772 (1985)

NOBUHARA, Yoshiko: "Protein determination by immunoblotting" Proceedings of the 25th Annual Meeting of Japan Society of Clinical Chemistry. 25. 90 (1985)

NOBUHARA, Yoshiko：“免疫印迹法测定蛋白质”第 25 届日本临床化学学会年会论文集。