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Practicability Studies on Continuous Monitoring of BloodConstituents

血液成分连续监测的实用性研究

基本信息

批准号：
60304085
负责人：
MURACHI Takashi
金额：
$ 6.46万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1987
项目状态：
已结题

项目摘要

Diagnosis of diseases is heavily dependent on the data from clinical laboratories, particularly those on the changes in the levels of biochemical constituents of the blood. The traditional way of access of these data is to collect blood samples from a patient at certain time intervals and to evaluate them together afterwards: this is to be called "discontinuous" method. In order to access the pathological changes more closely bound to the changing states of a patient, "continuous" monitoring of these blood constituents must be the method of better choice. We organized a group of co-operative studies for exploring the feasibility of applying biochemical analysis methods to such continuous monitoring. During the three-year term, 1985-1987, each one of us extended their own research related to clinical analysis, analytic method, data processing, etc., and in addition we have had group meetings twice every year and discussed on the mutually common problems. The followings are the major results obtained.(1) A bioreactor system for analysis which can be utilized for the continuous monitoring has been investigated. A unique chemiluminometric method for the determination of ammonia and urea has thus been developed. (2) Several biosensor systems such as a biosensor for beta-hydroxybutyrate, optical biosenor, etc., have been developed. (3) The practicability of continuous monitoring for the bed-side use or for the use during surgical operation has been discussed in a joint meeting on "Construction of Information for Diagnosis: Its hard- and Soft-wares," held in Tokyo on Februaty 5, 1988.

疾病的诊断在很大程度上依赖于临床实验室的数据，特别是血液生化成分水平变化的数据。获取这些数据的传统方法是在一定的时间间隔从患者采集血液样本，然后一起评估它们：这被称为“不连续”方法。为了更密切地了解与患者状态变化密切相关的病理变化，“连续”监测这些血液成分必须是更好的选择方法。我们组织了一组合作研究，探索将生化分析方法应用于这种连续监测的可行性。在1985年至1987年的三年任期内，我们每个人都拓展了自己在临床分析、分析方法、数据处理等方面的研究，此外每年还举行两次小组会议，讨论共同的问题。主要研究结果如下：(1)研究了一种可用于连续监测的分析生物反应器系统。因此开发了一种用于测定氨和尿素的独特的化学发光方法。 (2)开发了β-羟基丁酸生物传感器、光学生物传感器等多种生物传感器系统。 (3) 1988年2月5日在东京召开的“诊断信息建设：其硬件和软件”联席会议上讨论了床边使用或外科手术期间使用连续监测的实用性。