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Hepatic microcirculation and cellular energy level -Visualization of energy dependent processes in vivo-

肝脏微循环和细胞能量水平 -体内能量依赖过程的可视化-

基本信息

批准号：
60480210
负责人：
KAMADA Takenobu
金额：
$ 4.03万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60480210/
关键词：
hepatic microcirculation cellular energy level bile secretion in vivo epifluorescent microscope プロペディウムイオダイド FITC 蛍光顕微鏡

项目摘要

Cellular function needs energy expenditure, so that it depends on energy production. The energy conservation process usually needs the oxygen and the substrates which are supplied through microcirculation in tissues. Thus, the hepatic microcirculatory disturbance results in hepatic dysfunction. The pathogenesis and development of liver disease have been investigated from the aspect of hepatic microcirculation. In this study the relation of hepatic microcirculation with the cellular energy level was studied by visualizing the energy dependent biochemical processes using in vivo epifluorescent microscope system. The findings were as follows: 1)The bile secretion into bile duct in rats depended on regional hepatic tissue oxygenation and hepatic oxygen consumption analyzed by organ-tissue reflectance spectrophotometry. 2)Low concentration of ethanol increased the hepatic 02 consumption, while high concentration of ethanol suppressed the oxygen consumption which was associated with reductio … More n of respiratory chain cytochromes, and resulted in the decline of hepatic bile secretion. The bile secretion was correlated lineally with the hepatic energy charge (the adenine nucleotide being measured with high performance liquid chromatography). 3)In vivo epifluorescent microscope system with SIT camera and video processor was introduced to analyze the transport of fluorescent-Na from periportal to pericentral sinusoids and hepatocytes and from hepatocytes to bile canaliculi. In normal liver a homogeneous uptake by hepatocytes and polygonal appearance of bile canalicular network were observed, while in CC14-treated rats, a heterogeneous uptake of FITC and its secretion were observed, depending on cell damage and also microcirculatory disorders.In conclusion, visualization of energy-dependent processes in the liver in vivo such as bile secretion could be achieved with in vivo and in vitro epifluorescent and spectrophotometric microscope system with SIT camera and video processor. This technique was useful to visualize the physiological, pathological and biochemical processes occurring in the liver. Less

细胞的功能需要能量的消耗，因此它依赖于能量的产生。能量守恒过程通常需要通过组织内的微循环提供氧气和底物。因此，肝脏微循环障碍导致肝功能障碍。本文从肝脏微循环的角度探讨了肝病的发病机制和发展过程。本研究利用体内荧光显微镜系统对能量依赖性生化过程进行可视化观察，研究了肝脏微循环与细胞能量水平的关系。结果表明：1)大鼠胆汁分泌进入胆管依赖于肝组织局部氧合，用器官-组织反射分光光度法分析肝脏耗氧量。2)低浓度乙醇增加了肝脏02消耗，高浓度乙醇抑制了氧气消耗，呼吸链细胞色素减少，导致肝脏胆汁分泌下降。胆汁分泌与肝脏能量电荷呈线性相关（用高效液相色谱法测定腺嘌呤核苷酸）。3)采用带有SIT相机和视频处理器的体内荧光显微镜系统，分析荧光na从门静脉周围到中央窦周和肝细胞以及从肝细胞到胆管的转运。在正常肝脏中，观察到肝细胞的均匀摄取和胆小管网络的多边形外观，而在cc14治疗的大鼠中，观察到FITC的异质摄取及其分泌，这取决于细胞损伤和微循环障碍。综上所述，利用带有SIT相机和视频处理器的体内和体外荧光和分光光度显微镜系统，可以实现肝脏体内能量依赖过程（如胆汁分泌）的可视化。该技术有助于可视化肝脏中发生的生理、病理和生化过程。少