Research on positron emitting 62-zinc and 62-copper radiopharmaceuticals for Nuclear Medicine.

62-锌和62-铜是核医学中应用非常广泛的正电子发射核素；用62锌的母体制造62铜的发电机也是有道理的。在这项工作中，充分利用每一种金属的固有特性，进行了正电子发射放射性药物和62-铜发生器的开发，如下所述：1)基于62-锌放射性药物用于功能性和肿瘤性胰腺诊断。小鼠和狗的实验结果显示，如果作为62-锌的乙二胺-N，N'-二乙酸酯螯合物注射，62-锌在胰腺中的生物分布具有高度特异性；组织分析显示62-锌与羧肽酶A和b结合，在分泌素和CCK-PZ激素刺激下，62-锌通过胰管排泄增加；胰脏影像学检查显示了62-锌在胰脏内动员的有效性，进一步证明了62-锌螯合物作为外分泌功能诊断剂的适用性。此外，对荷瘤小鼠的筛选显示其在原发性癌诊断中的合理应用。2)探索开发62铜双功能螯合剂。基于以二硫代氨基脲双功能螯合剂99m-锝为金属配位所获得的有趣结果，合成了多种以不同活性胺基和脂肪酸为母体分子的62-铜螯合剂衍生物。它们的62-铜螯合物的稳定性和生物分布，保留了亲本分子的功能，反映在心肌和胰腺的特异性积累中，表明它们在功能诊断中的适用性。此外，有价值的数据是追求双功能的螯合剂的葡萄糖，作为母体分子。3) 62铜发电机的制造实验方法。筛选了62-锌的柱吸附原理及其子体62-铜的分离洗脱条件，为研制新型62-铜发生器奠定了基础。少

The 62-zinc and 62-copper are highly applicable and suitable positron emitting radionuclides in Nuclear Medicine; manufacturing of a 62-copper generator, from its 62-zinc parent is as well plausible. In this work, making the best use of intrinsic characteristics of each of those metals, the development of positron emitting radiopharmaceuticals and 62-copper generator were carried out, as next described:1) BASIS ON 62-ZINC RADIOPHARMACEUTICAL FOR FUNCTIONAL AND NEOPLASTIC PANCREAS DIAGNOSIS. Gathered results in experimental with mouse and dog, showed a highly specific biodistribution of 62-zinc in the pancreas, if injected as a 62-zinc chelate of ethylenediamine-N,N'-diacetate; the tissue analysis revealed the presence of 62-zinc bound to carboxypeptidase A and B. Under hormonal stimulation of secretin and CCK-PZ, an increased 62-zinc excretion through the pancreatic duct was achieved; validity of this 62-zinc mobilization in the pancreas was visualyzed by pancreas imaging studies, demo … More nstrating the applicability of 62-zinc chelate as an exocrine functional diagnostic agent; moreover, a screening with tumor bearing mouse revealed its plausible use for the diagnosis of primary carcinoma.2) SEARCH FOR THE DEVELOPMENT OF 62-COPPER BIFUNCTIONAL CHELATING AGENT. Based on interesting results achieved with 99m-technetium bifunctional chelating agent of dithiosemicarbazone as the metal coordination site, various of its 62-copper chelate derivatives holding diverse active amine groups and fatty acid as parent molecules were synthesized. Their 62-copper chelate stability and biodistribution with the preserved parent molecule functionality were reflected in the specific myocardial and pancreas accumulation, an indication of their applicability in functional diagnosis. Also, valuable data were pursued with a bifunctional chelating agent of glucose, as the parent molecule.3) EXPERIMENTAL APPROACHES ON THE MANUFACTURING OF A 62-COPPER GENERATOR. Screening of the principle governing the column adsorption of 62-zinc and the condition for the separation and elution of its daughter 62-copper set the basis for the development of a new 62-copper generator. Less