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Regulation of Gonadotrophins by the Non-Steroidal Substance Produced in the Ovary

卵巢产生的非甾体物质对促性腺激素的调节

基本信息

批准号：
60304036
负责人：
YOKOYAMA Akira
金额：
$ 4.22万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Co-operative Research (A)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60304036/
关键词：
Theca membrane Granulosa layer Inhibin Catecholamine Ovulation Oviposition Follicular growth 減数分裂

项目摘要

The purpose of this research is two fold: 1) To purify the non-steroidal substance produced in the ovary and to determine the biochemical and biological characteristics of this dubstance in regulating the secretion of gonadotrophins. 2) To compare the role of the substance in the follicular growing process and/or in ovulation in the mammal with that in the bird. Dr. Kosaku Tanaka extracted a heat labile, small molecular weight substance from the ruptured follicle in the chicken. This substance named "oviposition inducing factor (OOIF) was found to induce the release of arginine vasotocin. Dr. Shimada showed that prostaglandin contents in the thecal layer of the lst largest follicle reached maximum level at the time of ovulation. Dr. Yokoyama, in collaboration with Dr. Katuhide Tanaka, found that catecholamine contents increased before LH surge. Based on this finding, Dr. Tanaka incubated the granulosa cells with norepinephrine and/or LH and suggested the possibility that norepinephrine in the follicle of the chicken increased LH receptors in granulosa cells. Dr. Sato tried to purify 2 substances: The meiosis inhibiting substance and the ovum degeneration preventing substance. The latter substance that had been extracted from the follicular fluid of bovine follicles was found in the fraction of glycosaminoglycans. Dr. Sasamoto worked on the relationship between inhibin production and steroid production in the ovary and demonstrated that estrogen disappeared more quickly than inhibin after hypophysectomy in the rat.Two years are not long enough to accomplish two purposes. Although we are not able to deal with the 2nd purpose raised above, I am sure, our works on the ovarian substance make a great contribution to the endocrinological control of the gonads in birds and mammals.

本研究的目的有二：1）纯化卵巢产生的非甾体物质，并确定其调节促性腺激素分泌的生化和生物学特性。2)比较该物质在哺乳动物和鸟类卵泡生长过程和/或排卵中的作用。Kosaku Tanaka博士从鸡的破裂卵泡中提取了一种热不稳定的小分子量物质。这种物质被命名为产卵诱导因子（OOIF），它能诱导精氨酸催产素的释放。Shimada博士指出，第一个最大卵泡的卵泡膜层中的前列腺素含量在排卵时达到最高水平。Yokoyama博士与Katuhide Tanaka博士合作发现，在LH激增之前，儿茶酚胺含量增加。基于这一发现，Tanaka博士将颗粒细胞与去甲肾上腺素和/或LH一起孵育，并提出鸡卵泡中的去甲肾上腺素可能增加颗粒细胞中的LH受体。佐藤博士试图纯化两种物质：减数分裂抑制物质和卵子退化防止物质。从牛卵泡的卵泡液中提取的后一种物质存在于糖胺聚糖的部分中。Sasamoto博士研究了卵巢中雌激素产生和类固醇产生之间的关系，并证明了在大鼠垂体切除术后，雌激素比雌激素消失得更快。虽然我们不能处理上面提到的第二个目的，但我确信，我们对卵巢物质的研究对鸟类和哺乳动物性腺的内分泌控制做出了巨大贡献。