New Mechod on Evaluating Fibrinolytic Activity using Calormitry

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A novel method using calormetry on the evaluation of activity of fibrinolytic enzyme was developed. LKB microcaolrimeter equipped by flow-throug-cell was used. The protocol of the method was as follows: An enzyme and an excess amount of the substrate were mixed. The solution, in which the fibrinolytic reaction was in the steady state, was led to a thermodetector. The heat produced in unit time was measured. First, the reaction heat in two component system was measuted. Plasmin was used as a fibrinolytic enzyme. The reaction heat for fibrinogen, fibrin and casein as substrates of plasmin were measured. As fibrinogen showed the highest reaction heat, fibrinogen was adoptted as the substrate. Next, 3 component system i.e. urokinase, plasminogen and fibrinogen, was studied. Because of the practical 0 heat of the reaction from plasmininogen to plasmin, the heat observed in this system can be regraded as that of the reaction from fibrinogen to FDP by Plasmin. A linear relationship between UK concentration and observed heat was obtaind within the UK concentration of 0.5-20 unit/ml. Thus the method on evaluation of fibrinolitic activity using calorimetry was established. The methods using vescoelasticity and light transmittance were also studied.