New Mechod on Evaluating Fibrinolytic Activity using Calormitry

使用量热法评价纤溶活性的新方法

基本信息

  • 批准号:
    60870079
  • 负责人:
  • 金额:
    $ 1.79万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
  • 财政年份:
    1985
  • 资助国家:
    日本
  • 起止时间:
    1985 至 1987
  • 项目状态:
    已结题

项目摘要

A novel method using calormetry on the evaluation of activity of fibrinolytic enzyme was developed. LKB microcaolrimeter equipped by flow-throug-cell was used. The protocol of the method was as follows: An enzyme and an excess amount of the substrate were mixed. The solution, in which the fibrinolytic reaction was in the steady state, was led to a thermodetector. The heat produced in unit time was measured. First, the reaction heat in two component system was measuted. Plasmin was used as a fibrinolytic enzyme. The reaction heat for fibrinogen, fibrin and casein as substrates of plasmin were measured. As fibrinogen showed the highest reaction heat, fibrinogen was adoptted as the substrate. Next, 3 component system i.e. urokinase, plasminogen and fibrinogen, was studied. Because of the practical 0 heat of the reaction from plasmininogen to plasmin, the heat observed in this system can be regraded as that of the reaction from fibrinogen to FDP by Plasmin. A linear relationship between UK concentration and observed heat was obtaind within the UK concentration of 0.5-20 unit/ml. Thus the method on evaluation of fibrinolitic activity using calorimetry was established. The methods using vescoelasticity and light transmittance were also studied.
开发了一种使用量热法评估纤溶酶活性的新方法。使用配有流通池的LKB微量热量计。该方法的方案如下:将酶和过量的底物混合。将纤维蛋白溶解反应处于稳定状态的溶液引入热探测器。测量单位时间内产生的热量。首先,测量二组分体系中的反应热。纤溶酶用作纤维蛋白溶解酶。测量作为纤溶酶底物的纤维蛋白原、纤维蛋白和酪蛋白的反应热。由于纤维蛋白原显示出最高的反应热,因此采用纤维蛋白原作为底物。接下来,研究了三组分系统,即尿激酶、纤溶酶原和纤维蛋白原。由于从纤溶酶原到纤溶酶的反应热量实际上为 0,因此在该系统中观察到的热量可以重新分级为通过纤溶酶从纤维蛋白原到 FDP 的反应热量。 UK浓度在0.5-20单位/ml范围内,得到UK浓度与观察到的热量之间的线性关系。由此建立了量热法评价纤溶活性的方法。还研究了利用粘弹性和透光率的方法。

项目成果

期刊论文数量(14)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
中川輝昭,安田晶子,鳥居塚和生,足立伊左雄,上野雅晴,堀越勇: 病院薬学. 12. 369-372 (1986)
Teruaki Nakakawa、Akiko Yasuda、Kazuo Torizuka、Isao Adachi、Masaharu Ueno、Isamu Horikoshi:医院药理学。 12. 369-372 (1986)
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    0
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M.Ueno,M.Kimura,T.Nakagawa,Yasui,and I.Horikoshi.: Drug Interations. 11. 25-32 (1987)
M.Ueno、M.Kimura、T.Nakakawa、Yasui 和 I.Horikoshi.:药物相互作用。
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    0
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  • 通讯作者:
T. Nakagawa, A. Yasute, K. Toriizuka, I. Adachi, M. Ueno, and I. Horikoshi: "Measutement of Urokinase Activity Using Thrombelastgraph and Stability of Urokinase Preparation in Solution" Hospital Pharmacy. 12. 369-373 (1986)
T. Nakakawa、A. Yasute、K. Toriizuka、I. Adachi、M. Ueno 和 I. Horikoshi:“使用 Thrombelastgraph 测量尿激酶活性以及溶液中尿激酶制剂的稳定性”医院药房。
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    0
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  • 通讯作者:
M. Ueno, T. Nakagawa, Mn. Saito, and I. Horikoshi: "%development of the Mechod of Fibrinolytic Activity"
M. Ueno,T. Nakakawa,Mn。
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  • 影响因子:
    0
  • 作者:
  • 通讯作者:
中川輝昭,安田晶子,鳥居塚和生,足立伊左雄,上野雅晴,堀越勇: 病院薬学. 12. 369-373 (1986)
Teruaki Nakakawa、Akiko Yasuda、Kazuo Torizuka、Isao Adachi、Masaharu Ueno、Isamu Horikoshi:医院药理学。 12. 369-373 (1986)。
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UENO Masaharu其他文献

UENO Masaharu的其他文献

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{{ truncateString('UENO Masaharu', 18)}}的其他基金

Efficient synthesis of functional molecules by multicomponent tandem coupling reaction and application for flow synthesis
多组分串联偶联反应高效合成功能分子及其在流动合成中的应用
  • 批准号:
    19K05479
  • 财政年份:
    2019
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of the Continuous Microflow Reaction and Multi StepProcess
连续微流反应和多步工艺的发展
  • 批准号:
    23750099
  • 财政年份:
    2011
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Development of Catalyst Immobilized Micro Reactor for Combinatorial Multi Step Reactions
组合多步反应催化剂固定微反应器的研制
  • 批准号:
    21750091
  • 财政年份:
    2009
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Construction of influenza virosome and its development to preparation of vaccine
流感病毒体的构建及其疫苗制备的进展
  • 批准号:
    20590033
  • 财政年份:
    2008
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Studies on intermembrane protein transfer and establishment of preparation method of artificial membrane vaccine
人工膜疫苗膜间蛋白转移研究及制备方法的建立
  • 批准号:
    15590039
  • 财政年份:
    2003
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of New Preparation Method of Artificial Membrane Vaccine Using Spontaneous Transfer of Membrane Protein
利用膜蛋白自发转移制备人工膜疫苗新方法的开发
  • 批准号:
    07672311
  • 财政年份:
    1995
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Pilot Production of Artificial Membrane Vaccine
人工膜疫苗中试生产
  • 批准号:
    05807202
  • 财政年份:
    1993
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
Studies on the Factors Dominating Liposome Size, Size Distribution and Permeability
脂质体粒径、粒径分布及渗透性影响因素的研究
  • 批准号:
    60460218
  • 财政年份:
    1985
  • 资助金额:
    $ 1.79万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)

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Elucidation of the synovial pathology in osteoarthritis by focusing on the involvement of fibrinolytic activity and development of pain.
通过关注纤溶活性和疼痛发展的参与来阐明骨关节炎的滑膜病理学。
  • 批准号:
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纤溶活性降低的纯合纤溶酶原枥木突变小鼠的产生和表征
  • 批准号:
    25860801
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    2013
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Local fibrinolytic activity : an index of biological malignancy in digestive tract Cancers
局部纤溶活性:消化道癌症生物恶性肿瘤的指标
  • 批准号:
    04670776
  • 财政年份:
    1992
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EFFECT OF TRENTAL IN PLATELET AGGREGATION, FIBRINOLYTIC ACTIVITY
TRENTAL 对血小板聚集、纤溶活性的影响
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