Development of New Preparation Method of Artificial Membrane Vaccine Using Spontaneous Transfer of Membrane Protein

利用膜蛋白自发转移制备人工膜疫苗新方法的开发

• 批准号: 07672311
• 负责人: UENO Masaharu
• 金额: $ 1.47万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672311/
• 关键词: artificial membrane vaccine; membrane protein; influenza virus; liposomes; protein transfer; zeta potential; DMPC; membrane fluidity; 人口膜ワクチン; CVI細胞; SUV; ζポテンシャル; 表面構造; DMPC; 膜蛋白質

Spontaneous transfer of protein from either influenza virus-infected or uninfected cells to dimyristoylphosphatidylcholine (DMPC) liposomes was examined. The amount of transferred protein of liposomes incubated with influenza virus-infected cells (I-lipo) was more than that of liposomes incubated with uninfected cells (U-lipo). The ratio of the amount of spontaneous transfer protein of I-lipo to that of U-lipo increased in proportion to the diameter of liposomes except for small unilamellar vesicles. The amounts of transferred protein of negative-charged liposomes composed of DMPC and stearic acid or dicetylphosohate were larger than that of neutral DMPC liposomes. There was a correlation between zeta potential of liposomes and the contents of transferred protein. Membrane fluidity, however, was appeared not to be responsible for the enhancing effect of negative charged liposomes on spontaneous protein transfer.Therefore, we concluded that membrane surface structure based on electrostatic condition of liposomes will play an important role in the spontaneous protein transfer from cell membrane to recipient vesicles.

研究了蛋白质从流感病毒感染或未感染细胞到二肉豆蔻酰磷脂酰胆碱（DMPC）脂质体的自发转移。与流感病毒感染细胞孵育的脂质体(I-lipo)的转移蛋白量多于与未感染细胞孵育的脂质体(U-lipo)。除小单层囊泡外，I-lipo 与 U-lipo 的自发转移蛋白量之比与脂质体直径成比例增加。由DMPC与硬脂酸或二十六烷基磷酸组成的带负电的脂质体的转移蛋白量比中性DMPC脂质体的转移量大。脂质体的zeta电位与转移蛋白的含量存在相关性。然而，膜的流动性似乎并不是负电荷脂质体对自发蛋白质转移的增强作用的原因。因此，我们得出结论，基于脂质体静电条件的膜表面结构将在蛋白质从细胞膜到受体囊泡的自发转移中发挥重要作用。

项目成果

K. Kogure, O. Okuda T. Itoh K. Hayashi M. Ueno: "Development of Membraue Fusible Drug Cavrier from Erythroevte by the Spontanous Transtev of Uiral Fusion Protein from Inflneuza Uivas-Intected cells" Biol. Pharm. Bull.19 (in press). (1996)

K. Kogure、O. Okuda T. Itoh K. Hayashi M. Ueno：“通过 Inflneuza Uivas 感染细胞的 Uiral 融合蛋白的自发 Transtev 开发来自 Erythroevte 的 Membraue Fusible Drug Cavrier” Biol。

K.Kogure. M.Itoh,C.Nakamura.K.Hayashi,M.Ueno: "Progress in Drug Derivery system V ed by S.Hirota" Biomedical Research Foundation (Tokyo), 182 (1996)

K.小暮。

K.Kogure, M.Itoh, C.Nakamura, K.Hayashi and M.Ueno: "Effect of Memmbrane Surface Structure on Spontaneous Transfer of Protein from Membrane of Influenza Virus-Infected Cells to Liposomes" Progress in Drug Delivery System V, ed by S.Hirota, p71-74, Biomedi

K.Kogure、M.Itoh、C.Nakamura、K.Hayashi 和 M.Ueno：“膜表面结构对蛋白质从流感病毒感染细胞膜到脂质体自发转移的影响”药物输送系统 V 的进展，编辑

K.Kogure,M.Itoh,U.Kayashi,M.Ueno: "Spontaneous Transter of Viral Protein from Membrane of Intluenza Virus-Intecfed Cells to Liposomes is Dependent on the Diameter of Receiver" Biol. Pharm. Bull.18. 1168-1170 (1995)

K.Kogure、M.Itoh、U.Kayashi、M.Ueno：“病毒蛋白从流感病毒感染细胞膜到脂质体的自发转移取决于接收器的直径”Biol。

K. Kogure, M. Itoh, C. Nakamura K. Hayasi, M. Ueno: "Spoutaneous Transter of Vival Protein from Membrane of Influenza Vivus-Intected Cells to Liposomes in Dependent on the Pianeter of Receiver" Biol. Pharm. Bull.18 (8). 1168-1170 (1995)

K. Kogure、M. Itoh、C. Nakamura K. Hayasi、M. Ueno：“活体蛋白从流感病毒感染细胞膜自发转移到脂质体，取决于接受者的 Pianeter” Biol。