Pilot Production of Artificial Membrane Vaccine

人工膜疫苗中试生产

• 批准号: 05807202
• 负责人: UENO Masaharu
• 金额: $ 1.15万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05807202/
• 关键词: artificial membrane vaccine; MDP derivative; mixed vesicle; sodium cholate; detergent; influenza antigen; antigen protein; reconstitution; インフルエンザウイルス; 膜; B30-MDP; 界面活性剤除去法; 準弾性光散乱; 電子常磁性共鳴

A pilot production of artificial membrane vaccine was studied. First, we started a study on formation and property of mixed vesicles constituted of amphiphilic MDP derivatives and phosphatidylcholine. MDP (muramyl dipeptide) is the smallest unit of peptide glycan which possessed cell-mediated immunity and adjuvant activity. Mixed vesicles were prepared by detergent removal method. Mixed vesicles containing less than 40% of MDP derivatives with 2 hydrocarbon chains were unilamellar with 200 nm of diameter. Over 60% oligolamellar vesicles or fibriform structure apperared. The size of the vesicles containing a MDP derivative with a hydrocarbon chain was decreased with increasing MDP derivative content. Next we studied on reconstitution of influenza antigen protein on the vesicle membrane. The efficiency of the reconstitution of the antigen protein was the best when the vesicles were prepared by detergent removal method with sodium cholate as a surfactant.

研究了人工膜疫苗的中试生产。首先，我们开始研究由两亲性MDP衍生物和磷脂酰胆碱组成的混合囊泡的形成和性质。 MDP（胞壁酰二肽）是肽聚糖的最小单位，具有细胞介导的免疫和佐剂活性。采用去垢剂去除法制备混合囊泡。含有少于 40% 具有 2 个烃链的 MDP 衍生物的混合囊泡是单层的，直径为 200 nm。 60%以上出现寡片状囊泡或纤维状结构。含有具有烃链的MDP衍生物的囊泡的尺寸随着MDP衍生物含量的增加而减小。接下来我们研究了囊泡膜上流感抗原蛋白的重建。以胆酸钠为表面活性剂的去垢剂去除法制备囊泡时，抗原蛋白的重构效率最佳。

项目成果

Masaharu UENO,Chika MATSMOTO: "FORMATION AND PROPERTY OF MIXED VESICLES OF PHOSPHATIDYLCHCLINE AND MDP DERIVATIVES" Chem.Pharm.Bull.,. 41(11). 2066-2068 (1993)

Masaharu UENO、Chika MATSMOTO：“磷脂酰胆碱和 MDP 衍生物的混合囊泡的形成和性质”Chem.Pharm.Bull.,.

Masaharu UENO Chika MATSMOTO: "FORMATION AND PROPERTY OF MIXED VESICLES OF PHOSPHATIDYLCHOLINE AND MDP DERIVATIVES" Chem.Pharm.Bull.,. 41(11). 2066-2068 (1993)

Masaharu UENO Chika MATSMOTO：“磷脂酰胆碱和 MDP 衍生物的混合囊泡的形成和性质” Chem.Pharm.Bull.,.

Masaharu UENO and Chika MATSUMOYO: "FORMATION AND PROPERTY OF MIXED VESICLES OF PHOSPHATIDYLCHOLINE AND MDP DERIVATIVES" Chem.Pharm.Bull.41(11). 2066 (1993)

Masaharu UENO 和 Chika MATSUMOYO：“磷脂酰胆碱和 MDP 衍生物混合囊泡的形成和性质”Chem.Pharm.Bull.41(11)。

M.Ueno,C.Matsumoto: "Formation and Property of Mixed Micelles of Phosphatidylcholine and MDP Derivatives" Chem.Pharm.Bull.41. 2066-2068 (1993)

M.Ueno，C.Matsumoto：“磷脂酰胆碱和 MDP 衍生物混合胶束的形成和性质”Chem.Pharm.Bull.41。