Pilot Production of Artificial Membrane Vaccine

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A pilot production of artificial membrane vaccine was studied. First, we started a study on formation and property of mixed vesicles constituted of amphiphilic MDP derivatives and phosphatidylcholine. MDP (muramyl dipeptide) is the smallest unit of peptide glycan which possessed cell-mediated immunity and adjuvant activity. Mixed vesicles were prepared by detergent removal method. Mixed vesicles containing less than 40% of MDP derivatives with 2 hydrocarbon chains were unilamellar with 200 nm of diameter. Over 60% oligolamellar vesicles or fibriform structure apperared. The size of the vesicles containing a MDP derivative with a hydrocarbon chain was decreased with increasing MDP derivative content. Next we studied on reconstitution of influenza antigen protein on the vesicle membrane. The efficiency of the reconstitution of the antigen protein was the best when the vesicles were prepared by detergent removal method with sodium cholate as a surfactant.