Studies on the Factors Dominating Liposome Size, Size Distribution and Permeability

脂质体粒径、粒径分布及渗透性影响因素的研究

• 批准号: 60460218
• 负责人: UENO Masaharu
• 金额: $ 2.75万
• 依托单位: Toyama Medical & Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60460218/
• 关键词: Liposome; Detergent; Mixed Micelle; Diameter; Transient State; Time Factor; アンバライトXAD-2; リポソーム; 準弾性光散乱; 流体力学的半径; フリーズフラクチャー; 粒子径

Phospholipid vesicles, or liposomes were prepared by detergent removel using hydrophobic porous beads, Amberlite XAD-2, or dialysis from detergent-phospholipid mixed micelles. The liposomes formed were found to be mostly unilammellar vesicles. The liposome size was dependent on the methods of detergent removal and a type of detergents. Changes in forms of phospolipid-detergent mixture aggregates with decreasing detergent content-were pursuited by quasi elastic light scattering, gel-exclusion chromatography on Sephacryl S-1000, electron microscopy, and turbidity of the suspension. On the basis of the data, a possible model of the mechanism for transformation of the mixed micelle to liposome was proposed. The model is characterized by a chain-like aggregation entering an intermediate state before liposome formation. The liposome size and its distribution are dominated by the time factor in passing the transient state.

磷脂囊泡或脂质体通过使用疏水多孔珠、Amberlite XAD-2或从洗涤剂-磷脂混合胶束透析除去洗涤剂来制备。发现形成的脂质体主要是单层囊泡。脂质体的大小取决于去污剂的去除方法和去污剂的类型。磷脂洗涤剂混合物聚集体的形式的变化，随着洗涤剂含量的减少，追求准弹性光散射，凝胶排阻色谱Sephacryl S-1000，电子显微镜，和浊度的悬浮液。在此基础上，提出了混合胶束向脂质体转化的可能机理模型。该模型的特点是由一个链状聚集体进入脂质体形成之前的中间状态。脂质体大小及其分布受通过瞬态的时间因素支配。

项目成果

M. Ueno: Biochim. Biophys. Acta.904. 140-144 (1987)

M. Ueno：Biochim。

G.Gregoriadis;M.Ueno 他: "Liposomes as Drug Carriers:Recent Trends and Progress" John Wiley,

G. Gregoriadis；M. Ueno 等人：“脂质体作为药物载体：最新趋势和进展”John Wiley，

M. Ueno: "(review) Medical Application of Liposome" Med. & Pharm. J.22. 2045-2051 (1986)

M. Ueno：“（评论）脂质体的医学应用” Med。

M. Ueno: "Asymmetric Disposition of Detergents within Vesicle Bilayer and Its Effect on Ion Permeation through the Membrane" Biochim. Biophys. Acta.904. 140-144 (1987)

M. Ueno：“囊泡双层内洗涤剂的不对称分布及其对离子渗透通过膜的影响”Biochim。

M. Ueno: Hirokawa. Liposome-preparation, Property, Function, "Method on Bio-Colloidal Chemstry" ed. by S. Shimabayashi et al, (1988)

上野先生：广川。