Catecholoamines in urine: mass screening with microelectrode method.

尿液中儿茶酚胺：微电极法大规模筛查。

• 批准号: 60870097
• 负责人: KATO Takeshi
• 金额: $ 2.82万
• 依托单位: Gaduate School at Nagatsuta, Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1985
• 资助国家: 日本
• 起止时间: 1985 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-60870097/
• 关键词: Dopamini; In vitro voltammetry; neuroblastome; Pheocbromcytoma; マススクリーニング; 神経芽細胞腫; 尿; ニューロブラストーマ; ホモバニリン酸; 微小炭素電極; 高速液体クロマトグラフィー; セロトニン; 5HIAA

Determinations of monoamines in human urine are very important to find the patients with neuroblastoma or pheochromcytoma. In our recent research ( Clinical Chemistry, 1982), we inverstigated a method for the determination of monoamines is human urine using high-performance liquid chromatography with electrochemical detection. However, this method spend over 20 min to determine the monoamines for one sample.Recently, we developed a new technique using a carbon fiber electrode to determing dopamine in vitro. The catbon fiber electrode is connected with computer-controled DA/AD system. The sensitivity of the electrod is about 10^<-7>- 10^<-8>M. In the presence of ascorbic acid and dihydroxyphenylacetic acid (D@oPAC), 10^<-7>M of dopamine in the test tube could be detected.Although we applied this new technique to determine the concentration of dopamine in normal human urine, the sensitibity of the electrode was not not enough to determing the dopamine in the urine without any purification of it. Concentration of dopamine in the urine of neutoblastoms'a patinets may be 10 - 100 fold higher than that in the normal urine. Therefore, this new rechnique may be applicable to fins the patinets by a mass screening.

尿中单胺类物质的测定对神经母细胞瘤和嗜铬细胞瘤的发现有重要意义。在我们最近的研究（临床化学，1982年），我们研究了一种方法，用于测定单胺的人尿中使用高效液相色谱电化学检测。但是，这种方法测定一个样品的单胺需要20分钟以上。最近，我们发展了一种新的技术，使用碳纤维电极在体外测定多巴胺。碳纤维电极与计算机控制的DA/AD系统相连。电极的灵敏度约为10^<-7>- 10^<-8>M。在抗坏血酸和二羟苯乙酸（D@oPAC）存在下，<-7>可检测到试管中10 μ M的多巴胺，虽然我们用这种新技术测定了正常人尿中的多巴胺，但由于电极的灵敏度不够，不能直接测定未经纯化的尿中的多巴胺，中性母细胞瘤病人尿中的多巴胺浓度可能比正常人高10 ~ 100倍。因此，这种新的技术可能适用于鳍片的大规模筛选。

项目成果

Kayoko Ishii and Takeshi Kato: "Increase of dopamine turnover in bilateral striata after unilateral injection of haloperidol into substrantia nigra of unrtestrained rats" Brain Research. 359. 260-266 (1985)

Kayoko Ishii 和 Takeshi Kato：“向未受约束的大鼠黑质单侧注射氟哌啶醇后双侧纹状体多巴胺周转增加”大脑研究。

Mann Xu and Takeshi Kato: "Brain dialsys: changes in the activities of dopamine nuerons in rat striatum by perfusion of acetylcholine agonists under freely moving conditions" Neurochemistry Internationals.

Mann Xu 和 Takeshi Kato：“脑透析：在自由移动条件下灌注乙酰胆碱激动剂改变大鼠纹状体中多巴胺神经元的活动”神经化学国际。

加藤 武: 医学のあゆみ. 137. 879-882 (1986)

加藤武：医学史。137。879-882（1986）

Takeshi Kato;Bei Dong;Kayoko Ishii;Hiroyasu Kinemuchi: J.Neurochemistry. 46. 1277-1282 (1986)

加藤武；董北；石井佳代子；Kinemuchi 弘康：J.神经化学。

Hideko Yamamoto and Takeshi Kato: "Effects on brain cholecystokinin-8-sulfate and serotonin in adult rats after neonatal reduction of catechoamines" Neutoshience Research.

Hideko Yamamoto 和 Takeshi Kato：“新生儿儿茶胺减少后对成年大鼠脑胆囊收缩素-8-硫酸盐和血清素的影响”Neutoshience Research。