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Neuroscience research of the brain and its simulation: continuous measurements of monoamine contents in various brain regions under the unanesthetized conditions

脑神经科学研究及其模拟：非麻醉条件下连续测量脑各区单胺含量

基本信息

批准号：
61490012
负责人：
KATO Takeshi
金额：
$ 2.88万
依托单位：
Graduate School at Nagatsuta, Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Monoamine neurons in the brain might be related to the regulation of memory, learning, locomotion and other behaviours. Measurement of the monoamine contents in the brain are very important to know the phsiological activities of-the monoamine neurons. Previously, the content in the brain regions were determined by in vitro assay methods after killing the animals at an appropriate time. Since Killing the animals is not a good physiological condition, the data obtained by these methods do not show the real neuronal activities of the monoamines. In the present studies, we have developed 2 unique methods, in vivo voltammetry and brain dialysis, to determine the in vivo activities under the freely moving conditions.A newly developed voltammetry could detect dopamine as low as 2 nM in a test tube. Using this analytical system, dihydroxyphenylacetic acid, a metabolite of dopamine, were separated from dopamine.Brain dialysis method is a very useful technique to determine the in vivo release of neurotransmitters in the brain regions. Using this technique, we have found that nigro-striatal dopaminergic nerve terminals in rat striatum are regulated directly by nicotinic receptors and indirectly by muscarinic receptors. we have also established the assay method for the in vivo release of acetylcholine using the brain dialysis.

大脑中的单胺神经元可能与记忆、学习、运动和其他行为的调节有关。大脑中单胺含量的测量对于了解单胺神经元的生理活动非常重要。此前，在适当的时间处死动物后，通过体外测定方法测定大脑区域的含量。由于杀死动物并不是一个良好的生理条件，因此通过这些方法获得的数据并不能显示单胺的真实神经元活动。在目前的研究中，我们开发了两种独特的方法，体内伏安法和脑透析，以确定自由移动条件下的体内活动。新开发的伏安法可以检测试管中低至2 nM的多巴胺。使用该分析系统，将多巴胺的代谢产物二羟基苯乙酸从多巴胺中分离出来。脑透析方法是测定脑区域神经递质体内释放的非常有用的技术。利用这种技术，我们发现大鼠纹状体中的黑质纹状体多巴胺能神经末梢直接受烟碱受体调节，间接受毒蕈碱受体调节。我们还建立了脑透析体内乙酰胆碱释放的测定方法。