Monitoring more than 7 active substances in the brain microdialysate and the application in memory research

脑微透析液中7种以上活性物质的监测及其在记忆研究中的应用

• 批准号: 12558093
• 负责人: KATO Takeshi
• 金额: $ 1.92万
• 依托单位: Yokohama City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12558093/
• 关键词: monoamine; acetylcholine; microdialysis; neuropeptide; glia activation; Alzheimer disease model; HPLC-ECD; high sensitive assay; アルツハイマー病; 治療; マイクロダイアリシス; 電極; コリン; 痴呆症; ラクテート; HPLC; グルタミン酸; 神経伝達物質; 神経ペプチド; 記憶障害; ラット; ラット脳; 微小透析; NMD

1 There are catecholamine, 5HT, Ach, and so on of monoamines. This time, we established how to measure Ach sensitively. The Os-HRP-polymer, which is coated on Au-ring disk, is stable and useful for long time, then the electrode could detect a low amount of Ach in rat brain dialysate. Other monoamine also became possible in this method to measure it without use of the enzyme AchE into the brain perfusate. NE, DA, 5HT and those metabolites could be measured at the same time. Therefore, it actually became possible that more than seven kinds of nerve information products were measured at the same time. The purpose of this research subject could be attained.2.The various model animals of Alzheimer's dementia are proposed. In the present study, the degeneration of Ach neurons in rat NBM was used as the model. The cerebral cortex, hippocampus, and striatum of the rat were taken out on 7 day after NMDA receptor agonist ibotenic acid treatment into NBM. The neuropeptides of the cerebral cortex decreased, and the activation of glia cell was seen, too, those of which changes are seen in the brain of Alzheimer's dementia. This discovery is thought to contribute to the treatment for Alzheimer's dementia very much. As for the change in the neuropeptides found by this research, more research will be necessary to determine the release of the neuropeptides.

1.单胺类有儿茶酚胺、5-HT、Ach等。这一次，我们确定了如何灵敏地测量Ach。将Os-HRP聚合物包埋在金环片上，电极稳定性好，可长期使用，可检测到脑透析液中微量Ach。其他单胺也成为可能，在这种方法中，以测量它不使用酶乙酰胆碱酯酶到脑灌注液。NE、DA、5 HT及其代谢产物可同时测定。因此，实际上可以同时测量七种以上的神经信息产品。本课题的研究目的得以实现。2.提出了阿尔茨海默病痴呆的各种模型动物。本研究以大鼠NBM内Ach神经元的变性为模型。NMDA受体激动剂鹅膏蕈氨酸处理后第7天，取大鼠大脑皮质、海马和纹状体，注入NBM。大脑皮质神经肽减少，胶质细胞活化，这些变化在阿尔茨海默氏痴呆的脑中也有。这一发现被认为对阿尔茨海默氏症的治疗有很大贡献。至于这项研究发现的神经肽的变化，还需要更多的研究来确定神经肽的释放。

项目成果

K.Yamamoto et al.: "Solid-state pH ultramicrosensor based on -----"Anal. Chim. Acta. 480(1). 109-117 (2003)

K.Yamamoto 等人：“基于 ----- 的固态 pH 超微传感器”Anal。

T.Kato, K.Sato, K.Yamamoto, T.Chikuma: "Monitoring Molecules in Neuroscience. Proceedings of the 10th International Conference on In vivo Methods"Y.Kehr, K.Fuxe, U.Ungerstedt, T.Svensson (Karolinska Institute, Stockholm. Sweden). 274-275 (2003)

T.Kato、K.Sato、K.Yamamoto、T.Chikuma：“神经科学中的分子监测。第十届体内方法国际会议论文集”Y.Kehr、K.Fuxe、U.Ungerstedt、T.Svensson（卡罗林斯卡）

T.Chikuma et al.: "Fluorimetric assay for measuring Dns-His-Lys-Arg-His-Lys ---"Anal. Chim. Acta. 457(2). 157-164 (2002)

T.Chikuma 等人：“测量 Dns-His-Lys-Arg-His-Lys 的荧光测定法 ---”Anal。

R.Kurita et al.: "Microfluidic device integrated with pre-reactor ---"Sensors & Actuaors. 87(2). 296-303 (2002)

R.Kurita等人：“与预反应器集成的微流控装置---”传感器

L.Mao, G.Osborne, K.Yamamoto, T.Kato: "Continuous on-line measurement of cerebral hydrogen peroxide using enzyme-modified ring-disk plastic carbon film electrode."Anal.Chem.. 74. 3684-3689 (2002)

L.Mao、G.Osborne、K.Yamamoto、T.Kato：“使用酶改性环盘塑料碳膜电极连续在线测量脑过氧化氢。”Anal.Chem.. 74. 3684-3689（