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Development of the heat tolerant rat strain and its hybrids for physiological research of adaptaion

用于适应生理研究的耐热大鼠品系及其杂交种的开发

基本信息

批准号：
61870011
负责人：
FURUYAMA Fujiya
金额：
$ 2.56万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

The present project is consisted of three parts described below.(1) Development of a heat tolerant rat strain I had already a developing heat tolerant rat colony 3 years ago. Rats in the colony were selected in term of heat tolerance and inbred for further 3 years. The inbred strain has been developed. The inbred strain was most heat tolerant among 20 strains reported in 1982 and 1988. Heat tolerance of F1, F2 and bc between the inbred and ACI rats, which were one of heat intolerant strains, were determined for genetic analyses. Heat tolerance was inherited with polygenic control. Several main genes were suggested to contribute to thermoregulatory mechanisms in a hot environment. It is necessary to purify the inbred for further progress.(2) Development of hybrids Recombinant inbreds (RI) were established. The most RI were abolished because of temporary poor condition for research in the laboratory. Only one RI was remined and used for development of BC to the heat tolerant inbred. The BC is expected to be used as a control strain. Today, i begin to develop many RI for further progress.(3) Physiological research on heat tolerance The more heat tolerant the rat strain was, the more actively rats salivated in a hot environment and the higher the body water economics was. Colonic temperature of the heat tolerant rat was 1゜C lower in thermoneutral zone than the existing strains. Colonic temperature also was set near 40゜C in response to acute heat exposure.

本项目由下述三个部分组成。(1)耐热大鼠品系的开发3年前我已经有了一个正在开发的耐热大鼠群。对殖民地内的大鼠进行耐热性筛选，并进行3年的近交。已培育出近交系。在1982年和1988年报道的20个菌株中，自交系耐热性最强。本研究测定了近交系大鼠与不耐热品系ACI大鼠的F_1、F_2和bc的耐热性，并进行了遗传分析。耐热性遗传受多基因控制。几个主要的基因被认为有助于在热环境中的体温调节机制。为了进一步发展，有必要对近交系进行纯化。(2)杂交种的开发建立了重组自交系（RI）。由于实验室条件暂时较差，大多数研究所被取消。仅一个RI被扩增并用于将BC发育成耐热自交系。BC预期用作对照菌株。今天，我开始发展许多扶轮社为进一步的进展。(3)耐热性的生理学研究耐热性越强的品系大鼠在热环境中唾液分泌越活跃，体内水分经济性越高。在热中性区，耐热大鼠的结肠温度比现有品系低1 ℃。结肠温度也被设置为接近40摄氏度，以应对急性热暴露。