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Possible common mechanism for hot-and cold-resistance and gene analysis in FOK rat developed by us.

我们开发的FOK大鼠抗冷热可能的共同机制及基因分析。

基本信息

批准号：
16590176
负责人：
FURUYAMA Fujiya
金额：
$ 2.37万
依托单位：
Nagoya City University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590176/
关键词：
adaptation to environment global warming heat resistance lipid metabolism FOK rat thermoregulation DHA model animal 環境順応遺伝的モデル動物脂肪代謝熱産生

项目摘要

Physiological characteristics and gene analysis were studied in the FOK rat, for surveying common mechanisms for heat-and cold tolerance.(I) Changes in physiological characteristicsA. fat accumulationThe FOK rat was slightly obese at the age of 3 months and obese at 18 months.B. Further studies on blood lipid profileIt was reconfirmed that triglyceride was largely low in the FOK rat. Arachidonic acid increasedin it.Phospholipid increased up to 20%. DHA increased in phospholipid as those in the case of cold adaptation.C. Blood glucose value was normal at the age of 3months and 18 months.D. Possible effect of heat selection on trait of the FOK ratsSeveral FOK rats were abnormal. They has short face which was panda-like.Their body size was smaller than those in normal FOK rats. They were infertile.(II) Analysis of changes in genes in the FOK ratA. cDNA Microarray analysis in hypothalams in the FOK ratfirst analysis using a stress-related arrayseveral genes increased or decreased in hypothalamus were determined.Second analysis using an array for all genesMicroarray analysis was performed, however, clustering analysis was not yet.B. Quantitative Trait-related Loci Analysis (QTL analysis)Method for phenotype measurement was largely improved.An agreement was made with National Bioresource Project on identification of 350 gene markers.C. The FOK rat was deposiyyed to National Bioresource Project as a freezed oosperm.

研究FOK大鼠的生理特征和基因分析，以探讨耐热和耐寒的常见机制。(I)生理特性的变化A.脂肪积累FOK大鼠在3个月龄时轻度肥胖，在18个月龄时肥胖。血脂谱的进一步研究再次证实FOK大鼠的甘油三酯水平很低。花生四烯酸含量增加，磷脂含量增加20%。与冷适应时一样，DHA的磷脂含量增加. 3月龄和18月龄时血糖值正常。热选择对FOK大鼠性状的可能影响其面部短而似大熊猫，体型较正常FOK大鼠小。她们不能生育。(II)FOK大鼠基因变化分析A. FOK大鼠下丘脑cDNA微阵列分析第一次使用应激相关芯片分析，确定了下丘脑中几个基因的增加或减少，第二次使用所有基因的芯片分析进行了微阵列分析，但尚未进行聚类分析。与国家生物资源计划合作鉴定了350个基因标记，并在此基础上，进一步完善了水稻表型测定的QTL分析方法。FOK大鼠作为冷冻卵子保存在国家生物资源计划中。

项目成果

期刊论文数量（44）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Environmental enrichment brings a beneficial effect on beam walking and enhances the migration of doublecortin-positive cells following striatal lesions in rats

DOI：
10.1016/j.neuroscience.2006.10.038
发表时间：
2007-02-09
期刊：
NEUROSCIENCE
影响因子：
3.3
作者：
Urakawa, S.;Hida, H.;Nishino, H.
通讯作者：
Nishino, H.

RESPONSES OF THE INBRED FOK RAT TO AMBIENT HEAT AND COLD-In pursuit of common mechanisms to endure various stresses

近交系 FOK 大鼠对环境热和冷的反应——寻求承受各种应激的共同机制

DOI：
发表时间：
2005
期刊：
Adaptation Biology and Medicine vol 4 Current Concepts Vol.4
影响因子：
0
作者：
Murao H;Yoshida H;Nakahari T.他;Furuyama F
通讯作者：
Furuyama F

Deficiency of the tensin2 gene in the ICGN mouse : an animal model or congenital nephrotic syndrome.

ICGN 小鼠张力蛋白2基因缺陷：动物模型或先天性肾病综合征。

DOI：
发表时间：
2006
期刊：
Mamm Genome 17(5)
影响因子：
0
作者：
Shimamoto C;Fujiwara S;Kato M;Ito S;Katsu K;Mori H;Nakahari T.;尾仲達史;仲西未佳;Cho AR et al.
通讯作者：
Cho AR et al.

RESPONSES OF THE INBRED FOK RAT TO AMBIENT HEAT AND COLD-In pursuit of common mechanisms to endure various stresses-In : Adaptation Biology and Medicine

近交 FOK 大鼠对环境热和冷的反应 - 寻求承受各种压力的共同机制 - 在：适应生物学和医学