Possible common mechanism for hot-and cold-resistance and gene analysis in FOK rat developed by us.

对FOK大鼠的生理特性和基因分析进行了研究，以探索耐热和耐冷的共同机制。(1)生理特性的变化。脂肪堆积FOK大鼠在3个月大时轻度肥胖，18个月时肥胖。B.进一步的血脂谱研究再次证实，FOK大鼠的甘油三酯水平很低。花生四烯酸含量增加，磷脂含量增加高达20%。3.3月龄和18个月龄时血糖值正常；4.热选择对FOK大鼠生长性状的可能影响。它们的脸很短，很像熊猫，体型比正常的FOK大鼠小。它们都是不育的。(Ii)霍乱基因变异的分析。FOK大鼠下丘脑的cDNA微阵列分析首先利用应激相关序列分析下丘脑中几个基因的增加或减少。第二次使用全基因微阵列进行分析，但还没有进行聚类分析。B.用于表型测量的QTL分析方法得到了很大的改进。与国家生物资源计划的350个基因标记的鉴定达成了一致。C.FOK大鼠作为冷冻受精卵被安置到国家生物资源计划中。

Physiological characteristics and gene analysis were studied in the FOK rat, for surveying common mechanisms for heat-and cold tolerance.(I) Changes in physiological characteristicsA. fat accumulationThe FOK rat was slightly obese at the age of 3 months and obese at 18 months.B. Further studies on blood lipid profileIt was reconfirmed that triglyceride was largely low in the FOK rat. Arachidonic acid increasedin it.Phospholipid increased up to 20%. DHA increased in phospholipid as those in the case of cold adaptation.C. Blood glucose value was normal at the age of 3months and 18 months.D. Possible effect of heat selection on trait of the FOK ratsSeveral FOK rats were abnormal. They has short face which was panda-like.Their body size was smaller than those in normal FOK rats. They were infertile.(II) Analysis of changes in genes in the FOK ratA. cDNA Microarray analysis in hypothalams in the FOK ratfirst analysis using a stress-related arrayseveral genes increased or decreased in hypothalamus were determined.Second analysis using an array for all genesMicroarray analysis was performed, however, clustering analysis was not yet.B. Quantitative Trait-related Loci Analysis (QTL analysis)Method for phenotype measurement was largely improved.An agreement was made with National Bioresource Project on identification of 350 gene markers.C. The FOK rat was deposiyyed to National Bioresource Project as a freezed oosperm.