Development of Piezoelectric Immunosensor for the determination of Pyrogens

本研究旨在建立一种快速、灵敏的热原检测系统，利用压电石英晶体检测鲎试剂与热原之一的内毒素之间的凝胶反应。压电石英晶体被用来监测液体的粘度变化。粘度测量采用晶体等效电路中的电阻和阻抗分析仪测量的晶体谐振频率变化。内毒素的浓度是用该系统测定鲎细胞裂解液凝胶。胶凝时间是通过使用一个近似多项式方程从电阻或共振频率的变化计算。测定的胶凝时间与常规测定结果吻合较好。检出限为1 pg.ml-l。为了将测定时间缩短到小于等于bbb40 min，确定了最大变化率作为内毒素浓度的一个很好的指标。在此基础上，通过试验研制了压电石英晶体凝胶化检测器。用该仪器测定内毒素的结果与此相似。

The present study aims at the development of rapid and sensitive pyrogen assay system, using piezoelectric quartz crystal for the detection of gelation reaction between Limulus amebocyte lysate and endotoxin, one of the Pyrogens.A piezoelectric quartz crystal is used to monitor viscosity changes in a liquid. A resistance, included in the electrical equivalent circuit of the crystal, and the resonance frequency changes of the crystal, measured by an impedance analyzer, are used for the viscosity measurement. Endotoxin concentrations are determined with this system by the gelation of Limulus amebocyte lysate. The gelation time is computed by using an approximation to a polynomial equation from the resistance or the resonance frequency change. the gelation time measured was in good agreement with that obtained conventionally. The detection limit is 1 pg.ml-l. The maximum rate of change of the resistance was confirmed as a good index of endotoxin concentration in order to shorten the measurement time to <less than or equal> 40 min. The changes in the resistance or resonance frequency were also considered as indices of endotoxin concentrBased on these results, the 6-channel gelation detector using piezoelectric quartz crystals was manufactured by way of trial. With this apparatus, the similar results were obtained for the determination of endotoxin.