Development of micro-biosensors

生物传感器是一种新型的分析系统，可用于临床检测、工业过程控制和环境监测。最近，Karube教授的团队利用硅制造技术成功地实现了生物传感器的小型化和大规模生产。该项目旨在促进与法国和德国团体的合作研究。我们特别关注微生物传感器及其应用的发展。Tamiya留在布伦瑞克工业大学，研究了Schmidt教授小组新合成的人造脂质包覆压电晶体的气味传感器。田宫博士研究了稳定气味传感器的可能性。他和马蒂教授讨论过。佩皮尼昂大学关于农药传感器的研究。Marty教授的博士生将在Karube教授的实验室待1年半来推进这个项目。横山留在了保罗萨巴蒂尔大学的康塔特教授实验室。Comtat教授研究了电化学NAD回收系统，并将其应用于脱氢酶的生物传感器。横山博士利用固定化乳酸脱氢酶和脱氢酶的微型Pt电极制造了测定乳酸的微生物传感器。这是本次合作项目支持的实际产品之一。Suzuki研究了用于微生物传感器整合的固定化酶。Karube教授实验室使用的光交联聚合物可用于固定酶在布伦瑞克工业大学Schmidt教授实验室生产的多打印电极上。里昂大学的Coulet教授使用了一种简单的固定方法。只有浸泡在酶溶液中，膜和酶之间才能形成共价键。久保教授与贡比涅大学的Thomas教授进行了讨论，Thomas教授对控制生物反应器的传感器很感兴趣。小型化、集成化的生物传感器是监测生物反应器的有力工具。久保教授参观了佩皮尼昂大学马蒂教授的实验室，并讨论了用于环境分析的生物传感器。二氧化碳传感器是制造法国杀虫剂传感器所必需的。日本集团向法方提供二氧化碳传感器，以支持法方集团。Seki Madea与里昂大学的Coulet教授合作。他制作了基于LB膜的ISFET生物传感器。ISFET是一种非常有用的微生物传感器。LB技术可能是固定化生物材料的有力工具。佐藤和梶原也参与了这个项目。他们访问了贡比涅大学、保罗萨巴提大学和佩皮尼昂大学，以调查基于热探测的新型生物传感器的研究。Karube与Thomas教授和Schmidt教授讨论了这个项目的推广。他们的结论是，为了取得成果，这个项目应该继续下去。少

Biosensor is a novel analytical system which is available for clinical testing, industrial process control and environmental monitoring. Recently Prof. Karube's group has succeeded miniaturization and mass production of biosensors using silicon fabrication technology. This project aims at promotion of cooperational researches with French and Germany groups. We especially focus on development of micro-biosensors and their applications.Prof. Tamiya stayed at Braunschweig Institute Technology and investigated odorant sensor using piezoelectric crystal coated with artificial lipid which newly was synthesized by prof. Schmidt group. Dr. Tamiya examined possiblity of stable odorant sensor. He discussed with prof. Marty. Perpignan Univ. about pesticide sensor. Prof. Marty's doctor student will stay for 1 and half year at Prof. Karube's lab to promote this project.Dr. Yokoyama stayed at Prof. Comtat lab in Paul Sabatier University. Prof. Comtat has studied electrochemical NAD recyclng system a … More nd applied to biosensors using dehydrogenases. Dr. Yokoyama fabricated microbiosensors for determination of lactic acid using a miro Pt electrode immobilized lactate dehydrogenased and diaphorase. This ia one of actual products supported by this cooperational project.Dr. Suzuki investigated researches about immobilization of enzymes for integration of micro-biosensors. Photocrosslinking polymer used in prof. Karube lab is useful for immobilization of enzymes on multi-printed electrodes which are produced by Prof. Schmidt lab in Braunschweig Institute Technology. Prof. Coulet in Lyon Univ. used a simple immobilization method. Only immersion in enzyme solution gives covalent bonding between membrane and enzyme. Prof. Kubo discussed with Prof. Thomas in Compiegne Univ. who was interested in sensors for control of bioreactors. Minituarized and integrated bosensors are powerful tools for monitoring of bioreactors. Prof. Kubo visited Prof. Marty lab in Perpignan Univ. and discussed about biosensors for environmental analyses. Carbon dioxide sensor is requried for fabrication of French pecicide sensors. Japanese group supply carbon dioxide sensors to French side in order to support French group.Dr. Seki Madea colloboration with Prof. Coulet in Lyon University. He fabricated ISFET biosensor based on LB membrane. ISFET is one of useful transducers for micro-biosensors. LB techniques may be powerful tool for immobilization of biomaterials.Prof. Sato and Kajiwara also took parts in this project. They visited Compiegne Univ. Paul Sabatie Univ. and Perpignan Univ. in order to investigate researches about novel biosensors based on thermal detection.Prof. Karube discussed with Prof. Thomas and Prof. Schmidt about promotion of this project. They concluded that this project should be continued for making fruitful results. Less