Development of High Performance Biosensors

光生物传感器的发展：对光电极系统的基本性能进行了评价，并对其小型化及应用于临床或食品过程监测的可能性进行了探讨。对固定化酶(过氧化物酶)的检出限进行了估算，发现该光电极系统至少可以检测到4个皮摩尔的固定化酶。认为可以用平板型或雪崩型光电二极管代替光电倍增管作为光电探测器，从而使光电系统的小型化及其在临床和食品过程监测中的应用成为可能。高性能免疫传感器的开发：对于艾滋病或肝炎的诊断，需要高性能的免疫生物传感器。丹尼尔·托马斯教授一直在研究抗独特型催化抗体。因此，我们尝试将我们的微型器件作为传感器和它们的催化抗体相结合来开发高性能的…更多的Ce免疫生物传感器。在他们的实验室里，我们学习了如何制作和表征抗独特型催化抗体，以应用于免疫生物传感器。介质型生物传感器的发展：为了减少共存物质对反应的影响，需要介质型生物传感器。莫里斯·康塔特教授一直致力于光谱电化学方法的研究。用同步光谱分析和电化学方法研究了二茂铁羧酸(FC)修饰葡萄糖氧化酶的电极反应。分析表明，葡萄糖氧化酶通过二茂铁将电子转移到电极上。环境监测传感器的研制：本研究旨在构建快速、高灵敏的除草剂传感系统。乙酰胆碱酯酶和叶绿体可以作为对具有抑制作用的除草剂的良好传感装置。利用含有乙酰羟基酸合成酶基因的重组大肠杆菌建立了高效的乙酰羟基酸合成酶高产体系，用于磺脲类药物的检测。较少

Development of Photo Biosensor :Fundamental performance of the optrode system was evaluated, and the possibility of its miniaturization and application to clinical use or food process monitoring was investigated. The detection limit of the immobilized enzyme (peroxidase) was estimated and it was found that at least 4 pico moles of the immobilized enzyme could be detected by the optrode system. It was considered that a plate type or avalanche type photodiode could be used as a photodetector instead of the photomultiplier so that miniaturization of the optrode system and its application to clinical use or food process monitoring would be possible.Development of High Performance Immunosensor :For the diagnosis of AIDS or hepatitis, high performance immuno-biosensors are required. Prof.Daniel Thomas has been researching the anti-idiotypic catalytic antibody. Therefore, we have examined combining our micro-devices as the transducers and their catalytic antibody to develop the high performan … More ce immuno-biosensors. At their laboratory, we learned how to make and characterize the anti-idiotypic catalytic antibody for the application of the immuno-biosensors.Development of Mediator Type Biosensor :For the reduction of the effect of coexisting matters on the response, mediator type biosensors are required. Prof.Maurice Comtat has been working on spectroelectrochemical method. Electrode reaction of ferrocenecarboxylic acid (fc) modified glucose oxidase was characterized by simultaneous spectroscopic and electrochemical analysis. Electron transfer from glucose oxidase to the electrode via ferrocene was suggested from the analysis.Development of Environmental Monitoring Sensor :This study aimed at the fabrication of rapid and highly-sensitive herbicide sensing system. Acetylcholine esterase and chloroplast could be good sensing devices for herbicides which have inhibitory effects to those compounds. An efficient over production system of acetohydroxyacid synthase by using recombinant E.coli harboring its gene was also established to detect sulfonylureas. Less