Computational Methods for a Large Scale Annotation of Mitochondrial Genome

有丝分裂基因组是线粒体的基因组，线粒体是存在于大多数真核细胞中的细胞器。随着现代测序方法的发展，对大量物种进行有丝分裂基因组测序的研究已经成为可能。因此，已知有丝分裂组序列的物种数量正在迅速增长，并以越来越快的速度增长。用现有的计算方法不可能对所有可用的有丝分裂组数据进行全面和自动的分析。对于有丝分裂组的计算注释方法通常只是半自动的，或者只对某些类型的基因或有丝分裂组特征具有足够的质量，并且只对某些分类群具有足够的质量。此外，这些方法不足以快速注释大量的有丝分裂基因组序列。在这个项目中，我们想要为有效和全面的有丝分裂组序列注释建立算法基础，并想要展示所开发的方法如何有助于分析有丝分裂组的进化。我们还想研究开发的方法是否以及如何用于其他小基因组的注释，特别是对叶绿体基因组的注释。

Mitogenomes are the genomes of mitochondria which are organelles that are present in most eukaryotic cells. With modern sequencing methods research has become feasible that involves sequencing of mitogenomes for a large number of species. Therefore, the number of species for which the mitogenome sequence is known is growing rapidly and with increasing pace. A comprehensive and automatic analysis of all available mitogenome data is not possible with existing computational methods. Computational annotation methods for mitogenomes are often only semi-automatic or have a sufficient quality only for some types of genes or mitogenome features and only for certain taxa. Moreover, these methods are not efficient enough for a fast annotation of a large number of mitogenome sequences. In this project we want to develop the algorithmic foundation for an efficient and comprehensive annotation of mitogenome sequences and want to show how the developed methods can help for an analysis of the evolution of mitogenomes. We also want to study if and how the developed methods can be employed for an annotation of other small genomes, in particular for plastid genomes.