A micro-ring resonator as a DEP electrode: Bioparticle interaction phenomena and sensing integration issues

作为 DEP 电极的微环谐振器：生物粒子相互作用现象和传感集成问题

• 批准号: 404251289
• 负责人: Dr. Anders Henriksson
• 金额: --
• 依托单位: Fachgebiet Bioverfahrenstechnik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2018
• 资助国家: 德国
• 起止时间: 2017-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/404251289?language=en
• 关键词: micro; ring; resonator; DEP; electrode

Modern trends in bioanalytical research point toward interdisciplinary approaches and a miniaturization and digitalization that allows effective integration with modern microelectronics, and thus enable label-free automated sensors with highly specific molecular interactions. In the proposed project, photonic micro ring resonance sensing and dielectrophoresis (DEP) are fused together into a single microchip and combined with innovative click-based surface chemistry to control and examine molecular interactions and mass transfers. Innovative conducting mirroring resonators with retained optical properties and only a few 10 µm diameter will be used as the DEP electrode that focus the desired bioparticles directly onto the sensor surface. This will be realized thanks to a unique interdisciplinary constellation of BVT and IHP, allowing the production of professional semiconductor technology adapted to biological systems.The proposed portable device may bypass current limitation in biosensing attributed to molecular diffusion, thus allowing faster measurements and the potential to reach so far unattained sensitivities. Micro-ring resonance sensing may in addition allow to monitor the fundamental interaction between the free bioparticles and the electric field as well as to extract its dielectric properties. Further, by exciting the silicon micro-ring with TE and TM polarization it is possible to monitor structural changes of the immobilized probing molecules such as monolayer stretching and bending as well as induced conformational changes.The ability to focus bioparticles onto a single spot of an electrode will also be applied to explore and optimize innovative surface functionalization strategies and new methods for patterned immobilization of bioparticles. Our ambition with this project is not only to develop a new innovative sensor with extraordinary sensitivity but to lay a solid foundation, theoretically and practically aiming to simplify future integration of dielectrophoresis in molecular sensing.

生物分析研究的现代趋势指向跨学科方法和小型化和数字化，允许与现代微电子学有效集成，从而实现具有高度特异性分子相互作用的无标记自动化传感器。在拟议的项目中，光子微环共振传感和介电泳（DEP）融合在一起，形成一个单一的微芯片，并与创新的基于点击的表面化学相结合，以控制和检查分子相互作用和质量转移。创新的导电镜像谐振器具有保留的光学特性，直径仅为几个10 µm，将用作DEP电极，将所需的生物颗粒直接聚焦到传感器表面。这将是实现感谢一个独特的跨学科星座的BVT和IHP，允许生产专业的半导体技术，适应生物systems.The拟议的便携式设备可以绕过电流限制生物传感归因于分子扩散，从而允许更快的测量和潜力，达到迄今为止尚未达到的灵敏度。此外，微环共振感测可以允许监测自由生物颗粒与电场之间的基本相互作用以及提取其介电性质。此外，通过TE和TM极化激发硅微环，可以监测固定化探针分子的结构变化，如单层拉伸和弯曲以及诱导的构象变化。将生物颗粒聚焦到电极的单个点上的能力也将用于探索和优化创新的表面功能化策略和图案化固定生物颗粒的新方法。我们的目标不仅是开发一种具有非凡灵敏度的新型创新传感器，而且在理论和实践上奠定坚实的基础，旨在简化介电电泳在分子传感中的未来集成。