Service Project: Mass spectrometric proteomic and structural proteomics on CRISPR-Cas of archaea and bacteria

服务项目：古细菌和细菌的CRISPR-Cas质谱蛋白质组学和结构蛋白质组学

• 批准号: 405974719
• 负责人: Professor Dr. Henning Urlaub
• 金额: --
• 依托单位: Institut für Klinische Chemie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/405974719?language=en
• 关键词: Service; Project; Mass; spectrometric; proteomic

Mass spectrometry based proteomics has matured into a central technique to address analytical questions in cell biology and has extended its scope well into structural biology. It can provide answers to the state of protein populations in different cellular conditions both qualitatively and quantitatively. In particular, protein modifications can be assessed quantitatively, as well as protein-protein, protein-nucleic acids, and protein-ligand interactions can be resolved and elucidated at the amino acid level. This Z-project outlines mass spectrometric services provided to the SPP2141 project required to address questions on proteomics and structural biology regarding applications of CRISP-Cas systems in archae and eubacteria.

基于质谱学的蛋白质组学已经成熟成为解决细胞生物学中的分析问题的中心技术，并将其范围扩展到结构生物学。它可以定性和定量地回答不同细胞条件下蛋白质群体的状态。特别是，可以定量评估蛋白质修饰，也可以在氨基酸水平上解决和阐明蛋白质-蛋白质、蛋白质-核酸和蛋白质-配体之间的相互作用。这一Z-项目概述了为SPP2141项目提供的质谱学服务，这些服务需要解决有关蛋白质组学和结构生物学的问题，这些问题涉及CRISP-CAS系统在古细菌和真细菌中的应用。