Service Project: Mass spectrometric proteomic and structural proteomics on CRISPR-Cas of archaea and bacteria

以质谱法为基础的蛋白质组学已经成为解决细胞生物学分析问题的核心技术，并已将其范围很好地扩展到结构生物学。它可以从定性和定量上回答不同细胞条件下蛋白质种群的状态。特别是，可以定量评估蛋白质修饰，以及蛋白质-蛋白质，蛋白质-核酸和蛋白质-配体相互作用可以在氨基酸水平上解决和阐明。该z项目概述了SPP2141项目所需的质谱服务，以解决有关CRISP-Cas系统在古细菌和真细菌中的应用的蛋白质组学和结构生物学问题。

Mass spectrometry based proteomics has matured into a central technique to address analytical questions in cell biology and has extended its scope well into structural biology. It can provide answers to the state of protein populations in different cellular conditions both qualitatively and quantitatively. In particular, protein modifications can be assessed quantitatively, as well as protein-protein, protein-nucleic acids, and protein-ligand interactions can be resolved and elucidated at the amino acid level. This Z-project outlines mass spectrometric services provided to the SPP2141 project required to address questions on proteomics and structural biology regarding applications of CRISP-Cas systems in archae and eubacteria.