权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Establishment and application of the laboratory colony of Clethrionomys rufocanus bedfordiae susceptible to Echinococcus multilocularis for the antagonistic competion among taeniid species

多房棘球绦虫易感红毛密菌实验室种群的建立及其在绦虫类间拮抗竞争中的应用

基本信息

批准号：
61880009
负责人：
KAMIYA Masao
金额：
$ 6.98万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

Echinococcosis, also known as hydatid disease, is one of the most important cestode of man, domestic and wild animals. It is widely distributed in the world and recent information points to a spread of the disease into area previously free. In japan alveolar hydatid disease, caused by Echinococcus multilocularis, has further been reported in many hitherto Znon-endemicZ areas in Hokkaido in the past year, bringing the total number of endemic administrative districts to 121 in Hokkaido out of 212 in April 1988. The final goal of this project is the biological control of this parasite by the application of antagonistic competition between E. multilocularis and a closely related cestode in the laboratory-reared Clethrionomys rufocanus badfordiae.Four strains of E. multilocularis and 4 species with 8 strains of taeniid cestodes, i. e., Taenia taeniaeformis, T. crassiceps, T. polyacantha and T. hydatigena, have been isolated and maintained by serial passage in laboratory animals for this pur … More pose.The laoboratory colony of C. rufocanus bedfordiae has been established under the conditions of a temperature of 22 2゜c, a humidity of 55 5%, a ventilation of 10-15 times/hour with fresh air and 14 hours of lighting. The results showed that the gestation period was 18-22 days, the pregnancy rate was ca. 35%, the litter size averaged 5.1 1.6 and the weaning rate was 70-90%. The polymorphism of mitochondrial DNA among the populations and their serum isotypic immuno- globulines were caracterized.Antagonistic competion between closely related taeniid cestodes has been observed first by using the rat system, i. e., the strain of T. taeniaeformis -C. rufocanus origin which is resistant to rat can suppress completely the challenged infection of the rat susceptible strain in Wistar rats. Then, it has been confirmed that the number of established cycts of E. nultilocularis in the liver of C. rufocanus previously infected with C. rufocanus strain of T. taeniaeforis and of E. multilocularis alone show significant difference, i. e,. 16.9 4.4 and 35.0 15.0(p<0.05) for 2 weeks interval and 25.7 11.1 and 46.0 17.7(p<0.01) for 3 weeks interval of challenged infection, respectively. Less

棘球蚴病又称包虫病，是人类、家畜和野生动物最重要的绦虫之一。它在世界上广泛分布，最近的信息表明，该疾病已蔓延到以前没有的地区。在日本，由多房棘球绦虫引起的泡状棘球蚴病在过去一年中在北海道的许多迄今为止非地方病地区进一步报道，使得1988年4月北海道的212个行政区中的地方病行政区总数达到121个。本项目的最终目标是通过应用E.结果表明，在实验室饲养的红腹水龟（Clethrionomys rufocanus badforamus）中发现了多室水龟（E. multicellularis）和一种密切相关的绦虫。多房绦虫和带绦虫4种8株;例如，带形绦虫（Taenia taeniaeformis）、T. crassiceps，T. polyacantha和T.为了进行这种净化，已经分离并通过在实验室动物中连续传代来维持 ...更多信息 pose. C.在温度22.2 ℃、湿度55.5%、通风10-15次/h、光照14 h的条件下，建立了红腹小蠹的人工养殖场。结果表明，妊娠期18-22天，妊娠率约为100%。平均窝产仔数为5.1 ± 1.6只，断奶率为70- 90%。本文报道了不同带绦虫种群间线粒体DNA的多态性和血清同种型免疫球蛋白的特点。例如，T. taeniaeformis -C.对大鼠具有抗性的红藻能完全抑制Wistar大鼠对大鼠敏感品系的攻毒感染。证实了E. C. rufocanus以前感染过C. rufocanus菌株taeniaeforis和E.多房组差异有显著性，即：e，。16.9攻毒间隔2周分别为4.4和35.0 ± 15.0（p<0.05）;攻毒间隔3周分别为25.7 ± 11.1和46.0 ± 17.7（p<0.01）。少