Serological diagnosis of non-A, non-B hepatitis.

试图建立一种与非甲、非乙型肝炎相关的抗原抗体检测系统。我们用EV病毒转化法从恢复期的黑猩猩淋巴细胞中获得了单抗。我们在感染非甲、非乙型肝炎病毒的黑猩猩肝脏中发现了相应的抗原，而在感染甲型和乙型肝炎病毒的黑猩猩的肝脏中没有发现相应的抗原，但编码该抗原的cDNA与体细胞基因组杂交，表明该抗原不是病毒基因组编码的。这个日本克隆的丙型肝炎病毒与Chron的克隆序列不同。血液学在NS5区仅占74%。建立了丙型肝炎病毒抗体的EIA检测体系，并采用聚合酶链式反应的方法建立了丙型肝炎病毒基因组的cDNA检测体系。

An attempt was made to establish an antigen antibody assay system associated with non-A, non-B hepatitis. We have established monoclonal antibody from convalescent chimpanzee lymphocytes by EV virus transformation method. We found the corresponding antigen in the chimpanzee liver infected with non-A, non-B hepatitis virus and never found in the liver of the chimpanzees infected hepatitis A and B virus.However, cDNA coding this antigen hybridized with somatic genome, then this antigen was not coded by the viral genome.Then we cloned hepatitis C Genome from Japanese donor blood by immunoscreening and sequenced. Sequence of this Japanese clone of HCV is different from Chiron's clone. Hemology is only 74% in region NS5. We established EIA assay system of the antibody against HCV.And we also established cDNA assay system of HCV genom by PCR method.