Experimental Infection and Characterization of Epidemic Non-A, Non-B Hepatitis Virus.

尝试建立流行性非甲、非乙型肝炎动物模型。猕猴是一个很好的动物模型，因为如果我们静脉注射足够数量的病毒，几乎所有的猴子都患上了急性肝炎。串行传输实验也取得了成功。我们发现从接种后10天到转氨酶高峰，大量病毒排泄到胆汁中。早期病毒在胆汁中分散，下一阶段病毒颗粒自发聚集，可能是由于分泌IgA所致。我们已经用免疫筛选方法从lambda gt11 cDNA文库中克隆出病毒RNA。我们也用PCR法证实了病毒血症的潜伏期。现在我们正在制备重组肽抗体检测试剂盒。我们也在尝试开发重组疫苗。

An attempt was made to establish the animal model of epidemic non-A, non-B hepatitis. Cynomolgus macaca was a good animal model, because if we inoculate enough amount virus intravenously, almost all monkeys developed acute hepatits. Serial transmission experiments were also done successfully. We have found large amount of virus excrete into bile from 10 days after inoculation until peak of transaminase. In early stage, virus scattered in the bile but next stage virus particles spontaneously aggregated probably due to secretory IgA. We already cloned viral RNA from lambda gt11 cDNA library using immunoscreening method. We also confirmed viremia in incubation stage by PCR method. Now we are preparing antibody assay kit using recombinant peptide. We are attempting to debelop recombinant vaccine too.