Antigen-antibody system associated with non-A, non-B hepatitis.

试图建立与非甲、非乙型肝炎相关的抗原抗体系统。首先，我们纯化抗原，与非甲、非乙肝炎患者的恢复期血清反应，这些患者来自输血后的非甲、非乙肝炎患者的肝脏。该抗原被命名为AN6520抗原，并通过EIA建立了该抗原与抗体的测定体系。抗体在非甲、非乙型肝炎恢复期明显高流行。然而，进一步的研究发现这种抗原-抗体系统与非甲、非乙型肝炎没有直接关系。另一种尝试是用EB病毒转化的方法从恢复期黑猩猩身上克隆产生非a、非b型肝炎相关抗体的b淋巴细胞。我们建立了单克隆抗体定向微管聚集体，该聚集体被认为对非a、非b感染的黑猩猩具有特异性。抗原的纯化和检测系统的建立正在进行中。

An attempt was made to establish the antigen-antibody system associated with non-A, non-B hepatitis. First we purified antigen reacted with convalescent serum of the non-A, non-B hepatitis patients from the liver of a patient posttransfusion non-A, non-B hepatitis. This antigen designated as AN6520 antigen and established assay system of this antigen and antibody by EIA. Hi-prevalance of antibody in the convalescent srea of non-A, non-B hepatitis was evident. However, further investigation disclosed this antigen-antibody system does not dierctly associated with non-A, non-B hepatitis. The other attempt to clone the B-lymphocyte producing the antibody associated with non-A, non-B hepatitis from convalescent chimpanzee was made by EB virus transformation method. We established monoclonal antibody directed microtubular aggregate which is thought as specific for non-A, non-B infected chimpanzee. Purification of the antigen and establishment of assay system are ongoing.