Functional analysis of the novel podocyte-specific gene R3hdml for renal aging and progressive renal disease

新型足细胞特异性基因 R3hdml 对肾脏衰老和进行性肾病的功能分析

• 批准号: 415813655
• 负责人: Privatdozentin Dr. Wibke Bechtel-Walz
• 金额: --
• 依托单位: Universitätsklinikum Freiburg
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/415813655?language=en
• 关键词: Functional; analysis; novel; podocyte; specific

Here we aim to analyze the functional significance of the so far unknown protein R3hdml. R3hdml is a putative serine protease inhibitor that shows a podocyte-specific expression pattern during renal development. It was identified in a finger print expression analysis of murine podocytes and seems to be differentially regulated during renal aging and diabetic nephropathy (according to several GWAS studies). We generated a conditional R3hdml-knockout mouse line that will be further analyzed within this proposal. The animal studies have been accepted by the Regierungspräsidium. Additionally, we aim to investigate the functional relevance of R3hdml in in vitro studies of murine and human podocytes.

在这里，我们的目的是分析迄今未知的蛋白质R3hdml的功能意义。R3hdml是一种假定的丝氨酸蛋白酶抑制剂，在肾脏发育过程中显示足细胞特异性表达模式。它在鼠足细胞的指纹表达分析中被鉴定，并且似乎在肾老化和糖尿病肾病期间被差异调节（根据几项GWAS研究）。我们产生了条件性R3hdml敲除小鼠系，其将在本提案中进一步分析。动物研究已被Regierungspräsidium接受。此外，我们的目的是研究R3hdml在小鼠和人类足细胞的体外研究中的功能相关性。