Complement-mediated crosstalk between tubular and interstitial cells in renal transplantation - focus on the lectin-activated pathway (C02)

肾移植中补体介导的肾小管细胞和间质细胞之间的串扰 - 关注凝集素激活途径（C02）

• 批准号: 415865457
• 金额: --
• 依托单位: Pathologisches Institut
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/415865457?language=en
• 关键词: Complement; mediated; crosstalk; between; tubular

In this project, we will comprehensively characterize the activation of the complement pathway, in particular of the lectin pathway, during renal transplantation, with specific focus on the crosstalk of renal tubular epithelial cells and inflammatory cells. For this, we will use human renal biopsies and renal tissue from experimental studies in rats. Using either an inhibitory antibody directed against the activator of the lectin-activated complement pathway MASP-2, or a C5-blocker inhibiting the terminal route of all complement pathways, we aim to target complement activation to prevent graft deterioration and graft loss. In addition, interaction of various complement factors with renal tubular cells will be investigated in vitro.

在这个项目中，我们将全面表征肾移植过程中补体途径，特别是凝集素途径的激活，特别关注肾小管上皮细胞和炎症细胞的串扰。为此，我们将使用人肾活检和大鼠实验研究中的肾组织。使用针对凝集素活化的补体途径MASP-2的活化剂的抑制性抗体或抑制所有补体途径的末端途径的C5阻断剂，我们的目标是靶向补体活化以防止移植物劣化和移植物损失。此外，将在体外研究各种补体因子与肾小管细胞的相互作用。