歯の移動に伴う歯根膜線維の形態変化と歯槽骨改造過程の関連性

牙齿移动引起的牙周膜纤维形态变化与牙槽骨重塑过程的关系

• 批准号: 08771996
• 负责人: 岡藤 範正
• 金额: $ 0.64万
• 依托单位: Matsumoto Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-08771996/
• 关键词: 歯の移動; 歯根膜線維; 走査電顕; 初期変化; 圧迫側; 牽引側; 骨改造現象

本研究に先立ち,歯の移動に伴う移動後短期間の骨吸収と骨形成開始の様相を,走査電顕を用いて歯槽骨壁の広範囲を観察してきた.今回は,水酸化ナトリウムによって細胞成分を除去した非脱灰標本を用い,歯根膜線維の形状と形態を走査電顕を用いて観察し,歯の移動に伴う歯根膜線維と初期の骨改造現象との関連性について検討を行った.方法として,実験にはWistar系雄性ラット(250g【.+-。】20g)を使用し,上顎右側第一臼歯を,coil-springで初期荷重約10gで近心方向に牽引した.移動開始後より1週間まで経時的に屠殺し,5%グルタールアルデハイドにて固定した.その後,試料は非脱灰の状態で5%水酸化ナトリウム水溶液にて細胞成分を除去し,近遠心,頬舌側方向に割断し,カーボン,金二重蒸着後,走査電子顕微鏡(日本電子JCXA-733型)で観察した.結果として,圧迫側(近心面)では,移動数時間後より歯根膜の圧縮が歯頚側にみられ、経時的に根尖方向にもみられた.また歯根膜線維は屈曲し,歯槽骨壁付近で多数の血管腔を輪状に囲んだものや横断像等が認められた.移動24時間後には,屈曲した歯根膜線維に隣接した歯槽骨壁表面で,浅い吸収窩がみられ始め,この部位では歯根膜線維が断裂している像がみられ,吸収は経時的進行した.牽引側(遠心面)では,移動数時間後より歯槽頂線維束の歯根膜線維の伸展がみられ始め,経時的に根尖部においても伸展がみられた.移動24時間後には,吸収窩の窩底表面に石灰化構造物が次第に沈着しはじめ,これと同時に吸収窩付近の歯槽骨壁において歯根膜線維が確認され,伸展した歯根膜線維束の付近には細網線維と思われる網状構造も観察された.これらの歯根膜の伸展に伴う新しい骨の添加は経時的に増加傾向にあった.これより歯の移動に伴う骨改造は,歯槽骨表層に隣接した歯根膜線維の形態変化に密接な関係があることが示唆された.

In this study, we first set up the exercise. After the movement, the bone suction bone formation began to change in the short time after the movement, and the electrical equipment was used to measure the temperature of the groove bone wall. This time, the cell components of water acidification were used to remove the ash-free label, the shape of the root membrane, and the initial bone remodeling of the root membrane were observed by computer. Methods the male strain of Wistar was 250g [. + -. ] 20g) use the first mortar on the right, and the initial load of the coil-spring is about 10g near the center. After the start-up, the slaughterhouse is moved during the first half of the week, and 5% of it is fixed. After heating, the material is in a non-deashing state, acidified with 5% water, the aqueous solution is free of oxygen, near the center, the direction of the tongue is cut off, and the metal is double steamed, then go to the computer micrometer (Nippon computer JCXA- 733) to observe the temperature. Results the results showed that the pressure was increased, and the apical direction of the root membrane was significantly increased after several times of movement. The root membrane is flexion, and the alveolar bone wall is close to most of the vascular lumen. After 24 hours of movement, the buckling of the root membrane was connected to the surface of the groove bone wall, the surface of the surface of the grooves, the surface of the surface of the grooves, the surface of the grooves and the surface of the grooves. After several times of movement, the root membrane is stretched, and the tip of the root is stretched. After 24 hours of movement, the limed creation on the bottom surface of the machine is used for the first time, and at the same time, it is necessary to make sure that the root membrane is close to the bone wall of the groove. stretch the root film line to make sure that the network is close to the network. When the root membrane is stretched and the new bone is added, the stress is added to the new bone. The surface of the grooved bone is connected to the root membrane of the bone, and the surface of the grooved bone is connected to the root membrane of the bone.