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Single molecule imaging of architectural proteins during zebrafish embryo development

斑马鱼胚胎发育过程中结构蛋白的单分子成像

基本信息

批准号：
422780363
负责人：
Professor Dr. Christof Gebhardt
金额：
--
依托单位：
Institut für Biophysik
依托单位国家：
德国
项目类别：
Priority Programmes
财政年份：
2019
资助国家：
德国
起止时间：
2018-12-31 至 2023-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/422780363?language=en
关键词：
Single molecule imaging architectural proteins

项目摘要

The organization of chromatin in the nucleus of a cell is closely interlinked with functions such as gene expression, replication and DNA repair. In interphase, two important levels of organization exist within chromosome territories, separation of chromatin in active and inactive compartments and a network of self-associating chromatin loops. These loops are involved in transcription regulation by controlling the interaction between enhancer and promoter regions of chromatin. They arise and dissolve dynamically through the interplay of architectural proteins and their cofactors. How binding of architectural proteins to chromatin evolves in the course of early embryo development to mediate chromatin architecture is unclear.In this project, we want to study the evolution of interactions between architectural proteins and chromatin during early embryogenesis. In many model organisms such as Drosophila and zebrafish, this developmental period embraces the activation of zygotic transcription. We plan to record the kinetic behaviour of single architectural proteins by single molecule fluorescence imaging in live, developing zebrafish embryos during the time period of transcription activation. Thus we expect to obtain new insights into the functional interplay of chromatin architecture, gene transcription and architectural proteins.

细胞核中染色质的组织与基因表达、复制和DNA修复等功能密切相关。在间期，染色体区域内存在两个重要的组织水平，活性和非活性区室中的染色质分离以及自缔合染色质环网络。这些环通过控制染色质的增强子和启动子区域之间的相互作用参与转录调节。它们通过结构蛋白及其辅因子的相互作用动态地产生和溶解。在早期胚胎发育过程中，结构蛋白与染色质的结合如何演变以介导染色质结构尚不清楚。在本项目中，我们希望研究在早期胚胎发育过程中结构蛋白与染色质之间相互作用的演变。在许多模式生物，如果蝇和斑马鱼，这个发育期包括合子转录的激活。我们计划通过单分子荧光成像在活的、发育中的斑马鱼胚胎中记录转录激活期间单个结构蛋白的动力学行为。因此，我们希望获得新的见解染色质结构，基因转录和建筑蛋白质的功能相互作用。