Confocal laser microscope

共聚焦显微镜是免疫学研究中常用的标准技术，可对不同细胞群和组织进行详细的空间分析。具有多条激光线和快速扫描系统的现代共聚焦激光扫描显微镜可以分析固定和活标本中的细胞动力学、相互作用和形态。在不同的免疫学和神经免疫学研究问题中，共聚焦显微镜被应用于研究中枢神经系统（CNS）及其他系统中的免疫细胞群。用共聚焦显微镜研究CNS中的免疫细胞可以回答有关细胞群的不同方面，例如详细分析神经免疫疾病中的细胞募集或稳态和疾病期间常驻免疫细胞的行为。该领域的最新进展突出了在具有高度复杂性、动态性和可塑性的稳态和疾病期间CNS驻留和相关免疫细胞群体内迄今未探索的异质性。因此，迫切需要在研究所内规划和开发研究项目，以使用多种标记物精确识别细胞群，而且新报告模型的开发需要在一个标本内成像多个参数。

Confocal microscopy is a common standard technique in immunological research and offers a detailed spatial analysis of different cell populations and tissues. Modern confocal laser scanning microscopes with multiple laser lines and fast scanning systems allow the analysis of cell dynamics, interactions and morphology in fixed and living specimens. In different immunological and neuroimmunological research questions, confocal microscopy is applied to study the immune cell populations in the central nervous system (CNS) and beyond. Studying immune cells in the CNS with confocal microscopy allows to answer different aspects about the cell populations, such as a detailed analysis of cell recruitment in neuroimmunological disease or the behaviour of resident immune cells during homeostasis and disease. Recent advances in the field highlight a so far unexplored heterogeneity within the CNS-resident and –associated immune cell populations during homeostasis and disease with high complexity, dynamics, and plasticity. Therefore, there is an urgent need for planned and developing research projects within the institute to identify cell populations precisely with multiple markers, but also the development of new reporter models demands the imaging of multiple parameters within one specimen.