confocal STimulated Emission Depletion (STED) microscope system

共焦 ST 模拟发射损耗 (STED) 显微镜系统

• 批准号: 427466144
• 金额: --
• 依托单位: Christian-Albrechts-Universität zu Kiel
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/427466144?language=en
• 关键词: confocal; STimulated; Emission; Depletion; STED

In the Anatomical Institute are currently localized microscopes equipped with epifluorescence technology only. For the scientific progress of groups from the anatomical, biochemical and physiological departments a high demand on confocal microscopes with high resolution technology exists. Such a new technology is the STED-Stimulated Emission Depletion. It permits imaging with a resolution of low as 30 nm, and belongs to the high resolution technology with increasing demand. It enables the applicants to perform fluorescence imaging in extremely high quality of many subcellular structures and proteins and permits to solve many cell biological questions. The laser configuration of the applied STED microscope enables the imaging of up to eight different fluorochromes at the same time including its exact excitation, which permits versatile applications. At the university of Kiel no super resolution microscope exists. At the same time, a high demand across departments exists not only to support existing funded projects (f. e. SFB877 “Proteolysis as a Regulatory Event in Pathophysiology”, GRK2154 “Materials 4 Brain“ and various project fundings) but also to initiate new funded projects.

在解剖学研究所，目前只有局部显微镜配备了落射荧光技术。对于来自解剖学，生物化学和生理学部门的群体的科学进步，对具有高分辨率技术的共聚焦显微镜存在很高的需求。这种新技术是STED-受激发射损耗。它允许低至30 nm的分辨率成像，属于需求不断增长的高分辨率技术。它使申请人能够对许多亚细胞结构和蛋白质进行极高质量的荧光成像，并允许解决许多细胞生物学问题。应用STED显微镜的激光配置能够同时成像多达八种不同的荧光染料，包括其精确激发，这允许多功能应用。在基尔大学没有超分辨率显微镜存在。与此同时，各部门的高需求不仅是支持现有的资助项目（f）。e. SFB 877“Proteolysis as a Regulatory Event in Pathophysiology”、GRK 2154“Materials 4 Brain”和各种项目资助），而且还启动新的资助项目。