口腔癌の頸部リンパ節転移、肺転移に対する磁性微粒子を用いた磁場誘導組織内温熱療法

磁粒子磁场诱导组织内热疗治疗口腔癌颈淋巴结转移和肺转移

• 批准号: 16659550
• 负责人: 藤内 祝
• 金额: $ 1.98万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16659550/
• 关键词: VX7細胞; 転移モデル; Magnetoliposomes (MLs); 口腔癌; 温熱療法; Magnetoliposomes(MLs)

Magnetoliposomes (MLs)を用いた頚部リンパ節転移巣、肺転移巣に対する磁場誘導組織内温熱療法VX7舌癌転移モデルの舌に、粒子径約90nmのMLsをマイクロシリンジポンプを用い、30分かけて注入した。含有マグネタイトは20mgである。前年度の実験結果より、注入後のMLsの臓器移行は、深頚部部リンパ節においては注入後1日目、3日目でマグネタイト量は最大を示した。肺転移巣に関しては、移行マグネタイト量は微量であり、基礎実験における、磁場下での温熱治療効果を認める温度まで加温するためのマグネタイトの集積は肺転移巣には認めなかった。転移モデルの舌へのMLs注入後、1日目に、頚部に対して限局的に高周波磁場を照射した。温度測定は、経皮的に頚部リンパ節に刺入した光フィアバープローブを用いて行った。転移巣内部の温度は磁場照射より10分後に温熱効果のあるとされる42.5度に達し、以後磁場照射中はそれ以上の温度を維持することが可能であった。加熱は原則的に42.5度を示してから30分間施行した。温熱治療施行より1日目に、頚部リンパ節を摘出し本治療法による転移頚部リンパ節に対する治療効果を病理組織学的に検討した。免疫組織科学染色の結果、加温後頚部リンパ節はアポトーシス陽性を示し、一部腫瘍細胞はネクローシスも認めた。一回の加温では、残存腫瘍細胞も存在していた。抗腫瘍効果に対する検討はまだ行っていないが、繰り返し加温により残存腫瘍細胞の消失も十分期待できる。肺転移に対しては、舌へのMLsの注入後1日目のMRI画像において、マグネタイトの肺転移巣への集積を認め、画像検査におけるMLsの有効性が示唆された。肺転移巣への温熱効果に関しては現段階では困難であった。

Magnetoliposomes (MLs) was used to determine the temperature in the tissue of VX7 tongue cancer by using the magnetic field method of tissue temperature measurement. The diameter of the particles was about 90nm MLs and injected into the tissue. It contains the following words: "20mg"." The results of the previous year, the transfer of the MLs device after the injection, and the maximum volume of the first and third days after the injection. The lungs were transferred to the lungs, the lungs were moved, the lungs were moved, the lungs were measured, and the temperature was warmed up by warming the lungs. After the injection of MLs, the high-frequency magnetic radiation of the limited office was observed on 1 day. The temperature is measured and the temperature is measured. The surface of the skin is pierced. After 10 minutes of exposure to the internal temperature magnetic field, the temperature will increase to 42.5 degrees Celsius, and the temperature will be maintained above the temperature during the subsequent magnetic field irradiation. An additional policy of 42.5 degrees indicates that the policy will be implemented within 30 minutes of the clock. Wen Zhi-Zhi carried out the treatment of the disease in the first day, and then extracted the pathological histology of the pathology of the disease. After heating, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue, the results of scientific staining of immune tissue and the results of scientific staining of immune tissue. Once the temperature is heated, there is a cell in which the remaining cells are heated. The results show that the cells are not allowed to disappear and are very much expected to disappear. One day after the injection of lung movement and tongue MLs, the MRI portrait showed that there was a sexual abstinence. The lungs were moved and warmed up so that there was no sign of pain in the body.

项目成果

Heat immunotherapy using magnetic nanoparticles and dendritic cells for T-lymphoma.

• DOI: 10.1263/jbb.100.112
• 发表时间: 2005-07
• 期刊: Journal of bioscience and bioengineering
• 影响因子: 2.8
• 作者: Kouji Tanaka;A. Ito;Takeshi Kobayashi;T. Kawamura;S. Shimada;Kazuhiko Matsumoto;T. Saida;H. Honda

A mechanism of antitumor immunity induced by hyperthermia

热疗诱导抗肿瘤免疫的机制

• 发表时间: 2005
• 期刊: Japanese Journal of Hyperthermic Oncology 21・1
• 作者: Nishigawa;G.;et al.;Akira Ito
• 通讯作者: Akira Ito

Complete regression of hereditary melanoma in a mouse model by repeated hyperthermia using magnetite cationic liposomes

使用磁铁矿阳离子脂质体重复热疗使小鼠模型中的遗传性黑色素瘤完全消退

• 发表时间: 2005
• 期刊: Japanese Journal of Hyperthermic Oncology 21・3
• 作者: Nishigawa;G.;et al.;Akira Ito;Kouji Tanaka;Kouji Tanaka;Akira Ito
• 通讯作者: Akira Ito

Intratumoral injection of immature cells enhances antitumor effect of hyperthermia using magnetic nanoparticles.

肿瘤内注射未成熟细胞可增强磁性纳米粒子热疗的抗肿瘤效果。

• 发表时间: 2005
• 期刊: Int J Cancer 116(4)
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• 通讯作者: Tanaka K.( et al.