一酸化窒素による細胞内微量元素分布の変動が肝細胞がん増殖に与える影響に関する研究

一氧化氮改变细胞内微量元素分布对肝癌生长影响的研究

• 批准号: 17659209
• 负责人: 松崎 靖司
• 金额: $ 2.11万
• 依托单位: Tokyo Medical University (2006)University of Tsukuba (2005)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Exploratory Research
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17659209/
• 关键词: 胃がん細胞; 胃粘膜細胞; 一酸化窒素; 微量元素; SOD; Ribonuleotide Reductase; 鉄; がん; NO

消化器由来の培養細胞として、ラット正常胃粘膜由来のRGM-1と、ヒト胃がん由来のMKN-45を用いた。1)各群の細胞数の倍加時間を測定し、一定時間でのBrdUの取り込み量を生体蛍光顕微鏡と画像処理装置を組み合わせた装置で測定しDNA複製速度を比較したところ、正常胃粘膜細胞であるRGM-1と胃がん由来MKN-45細胞との間に有意な差を認めなかった。2)細胞内NO, ONOO-濃度をNO特異的蛍光指示薬DAF2DAおよび活性酸素種蛍光指示薬HPF/APFの組み合わせで得られる細胞の蛍光を上記測定装置によって計測したところ、胃がん細胞由来のMKN-45において、有意に双方の蛍光強度の上昇を認めた。がん細胞において細胞内NO・ONOO-量が上昇していることが確認された。3)細胞内iNOS, SOD発現を抗iNOS,抗Zn/Cu-SODモノクローナル抗体で免疫組織学的に検討したところ、RGM-1ではともに発現が認められなかった。4)核におけるRibonuleotide Reductase(RNR)発現を抗RNRモノクローナル抗体で免疫組織学的染色し確認したが、両細胞間で明らかな差は認められなかった。核分画を抽出して同様の実験を行ったが、有意差を認めなかった。5)ヘマトヘミン-59Feを用い、細胞培養液中に存在する鉄の取り込み量がRGM-1細胞とMKN-45細胞で差があるのか検討したが、鉄取り込み量に有意差は認められなかった。

Cultured cells derived from digestive organs are used in RGM-1 and MKN-45 derived from normal gastric mucosa. 1)The number of cells in each group was measured at a certain time, and the amount of BrdU was measured at a certain time. The speed of DNA replication was measured at a certain time. The normal gastric mucosa cells were compared with the normal gastric mucosa cells. The origin of MKN-45 cells was determined at a certain time. 2)Intracellular NO, ONO-concentration, NO-specific fluorescence indicator DAF2DA, and active acid species fluorescence indicator HPF/APF combination were used to measure the fluorescence intensity of the cells in the stomach and MKN-45 cell origin. NO·ONOO-in the cells increased and was confirmed. 3)Intracellular iNOS, SOD expression Anti-iNOS, anti-Zn/Cu-SOD anti-iNOS, anti-Zn/Cu-Zn/Cu-SOD anti-iNOS, anti-Zn/Cu- 4)Nuclear Ribonuleotide Reductase(RNR) was detected, anti-RNR antibodies were detected, and immunohistochemical staining was performed to confirm the presence of RNR. We're going to have to do something about it. We're going to have to do something about it. 5) The presence of iron in cell culture medium and the concentration of iron in RGM-1 cells and MKN-45 cells were significantly different.

项目成果

Apoptosis and inhibition of the phosphatidylinositol 3-kinase/Akt signaling pathway in the anti-proliferative actions of dehydroepiandrosterone

• DOI: 10.1007/s00535-005-1574-3
• 发表时间: 2005-05-01
• 期刊: JOURNAL OF GASTROENTEROLOGY
• 影响因子: 6.3
• 作者: Jiang, YF;Miyazaki, T;Matsuzaki, Y
• 通讯作者: Matsuzaki, Y

Suppressive effect of ursodeoxycholic acid on type IIA phospholipase A2 expression in HepG2 cells.

熊去氧胆酸对 HepG2 细胞中 IIA 型磷脂酶 A2 表达的抑制作用。

• DOI: 10.1002/hep.20630
• 发表时间: 2005
• 期刊: Hepatology (Baltimore, Md.)
• 作者: Ikegami,Tadashi;Matsuzaki,Yasushi;Fukushima,Sugano;Shoda,Junichi;Olivier,JeanLuc;Bouscarel,Bernard;Tanaka,Naomi
• 通讯作者: Tanaka,Naomi

Role of the nrf-2 gene in protection and repair of gastric mucosa against oxidative stress

• DOI: 10.1163/156856005774423863
• 发表时间: 2006
• 期刊: InflammoPharmacology
• 影响因子: 5.8
• 作者: A. Yanaka;Songhua Zhang;Masafumi Tauchi;Hideo Suzuki;T. Shibahara;Hirofumi Matsui;Akira Nakahara;N. Tanaka;Masayuki Yamamoto

Enhancement of DNA topoisomerase I inhibitor-induced apoptosis by ursodeoxycholic acid

• DOI: 10.1158/1535-7163.mct-05-0107
• 发表时间: 2006-01-01
• 期刊: MOLECULAR CANCER THERAPEUTICS
• 影响因子: 5.7
• 作者: Ikegami, T;Matsuzaki, Y;Bouscarel, B
• 通讯作者: Bouscarel, B

Involvement of integrin‐linked kinase in carbon tetrachloride–induced hepatic fibrosis in rats

• DOI: 10.1002/hep.21315
• 发表时间: 2006-09
• 期刊: Hepatology
• 影响因子: 13.5
• 作者: Yining Zhang;T. Ikegami;A. Honda;T. Miyazaki;B. Bouscarel;M. Rojkind;I. Hyodo;Y. Matsuzaki