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ゲノム・ポストゲノムリソースを利用したトマトの二次代謝産物トランスポーターの解析

利用基因组和后基因组资源分析番茄次生代谢转运蛋白

基本信息

批准号：
18658010
负责人：
白武勝裕
金额：
$ 2.05万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Exploratory Research
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2008
项目状态：
已结题

项目摘要

植物において二次代謝産物の分泌・蓄積やホルモンの輸送に関わるABC輸送体に着目し,トマトのポストゲノムリソースを利用することにより,園芸作物におけるABC輸送体の機能を明らかにすることを目標とした.1, トマトABC輸送体遺伝子のクローニングと生化学的性質の解析かずさDNA研究所のESTリソースから得た10種のABC輸送体cDNAクローンのうち,3種は完全長であったが,7種は5'または3'領域が欠けており,このうちの2種の完全長cDNAをRT-PCRまたはRACE法によりクローニングした.完全長の得られた5種のABC輸送体の器官別およびストレス処理時における遺伝子発現を,リアルタイムPCR法により調べた.その結果,アントシアンなどの二次代謝産物の輸送に関わることが予想されるSlMRP1の発現が成熟果実で顕著に高いこと,テルペノイドなどの二次代謝産物の輸送を担うことが予想されるSlPDR1の発現は果実の成長期間を通して比較的高いこと,また,オーキシンを輸送することが予想されるSlMDR1が着果直後の果実で顕著に高いことが明らかとなった.一方,ワックスの輸送に関わることが予想されるSlWBC1およびSlWBC2の発現はどの器官でも比較的恒常的であった.2,ABC輸送体の形質転換体の作出果実の成長期間を通して比較的発現が高かく,テルペノイドなどの二次代謝産物の輸送を担うことが予想されるSlPDR1の発現を,RNAiにより抑制した形質転換トマトを作出したところ,葉の表面に多量の分泌物が確認された.現在,その二次代謝産物の組成を野生型と比較し,SlPDR1の輸送基質の推定を進めている.

The second generation of plant products is secreted and stored, and the ABC is sent to the body to look at the object. This is the best way to make use of the production of the plant. The plant plant is responsible for the second generation of the plant. The second generation of the plant product is secreted and stored in the second generation of the plant. The second generation of the plant product is secreted and stored in the plant. The second generation of the plant product is secreted and stored in the second generation of the plant. An analysis of the characteristics of biochemistry in the field of biochemistry. The DNA Research Institute has received 10-year-old ABC delivery of cDNA, 3-year-old, 5-year-old, 3-year-old, 3-year-old, 5-year-old, 3-year-old, 3-year-old, 5-year-old, 5-year-old, 3-year-old, 5-year-old, 3-year-old, 5-year-old The fully grown ABC donated organs can be detected during the operation, and the PCR method can be used to detect the temperature. According to the results, the second generation of the product is expected to improve the quality of the mature fruit of the SlMRP1. The results show that the second generation of the product is responsible for the improvement of the quality of the product. The results show that you want to improve the performance of the second generation. The results show that you want to improve the quality of the mature fruit. The results show that you want to improve the long-term performance of the SlPDR1. Please tell me that you want to SlMDR1 the fruit and then you can tell me that it is a good idea. On the other hand, you know, on the other hand, you know, you know, on the other hand, you know, on the other hand, you know, you know, you know RNAi inhibits the appearance of a large amount of secretions on the surface of the body to make sure that it is not clear. At present, the second generation is composed of wild-type antigens, and the SlPDR1 is presumed to be in progress.