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Analysis of Neural Circuit by Means of Automatic Trail Recording System

自动轨迹记录系统分析神经回路

基本信息

批准号：
01480021
负责人：
HISADA Mituhiko
金额：
$ 1.28万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Male cricket Gryllus bimaculatus sings his calling song to attract the conspecific female to facilitate their mating. To clarify the neural circuit for female cricket's approaching behavior in response to male's calling song, we have attempted to define the connections of central neurones using the combination of ordinary intracellular recording techniques with a newly devised open loop Kramer's sphere.Motor neurnes which innervate hind leg muscles were identified both physiologically and morphologically in the metathoracic ganglion of the female crickets. The relative position of cell bodies of about 30 leg motor neurones were mapped. Both spiking and nonspiking local interneurnes were found to affect the activity of leg motor neurones. Some nonspiking local interneurnes seemed to receive inputs from auditory descending interneurones. The preliminary study which records these central neurones intracellularly during female's approaching behavior was performed.The observations of attracted behavior of female crickets were performed with an automatic trail recording system. The system (Kramer's sphere) consists of an air floated plastic sphere which serves as a treadmill. A cricket to be tested is placed on the top of sphere. Rotation of the sphere is monitored by a non-contact photo-sensor placed on the opposite pole of the sphere to the animal. The photo-sensor detects the change in albedo of the sphere due to the ralation along the x and y axes of right angle to the later reconstruction of the traces. The sensor converts the albedo change and direction of the rotation into a set of digital recording with a sequence detector circuit. A personal computer automatically read and store the data acquired in the form of a file. Direction of the orientation in angle and approaching behavior for the distance and the speed are calculated and displayed for the evaluation.

雄性蟋蟀会用歌声来吸引同种的雌性蟋蟀进行交配。为了阐明雌蟋蟀对雄蟋蟀鸣叫的接近行为的神经回路，我们尝试用普通的细胞内记录技术和新设计的开环克雷默球相结合的方法来确定中枢神经元的连接，从生理和形态学上鉴定了支配后肢肌肉的运动神经元。绘制了约30个下肢运动神经元胞体的相对位置图。尖峰和nonspiking本地interneurnes被发现影响腿部运动神经元的活动。一些nonspiking本地interneurones似乎收到的听觉下行interneurones的输入。本研究对雌蟋蟀接近行为过程中的这些中枢神经元进行了细胞内记录的初步研究，并利用自动跟踪记录系统对雌蟋蟀的引诱行为进行了观察。该系统（克雷默的球）包括一个空气浮动的塑料球，作为跑步机。一只待测试的蟋蟀被放置在球体的顶部。球体的旋转由放置在球体与动物相反的极上的非接触式光电传感器监测。光电传感器检测由于与稍后的轨迹重建成直角的沿着x和y轴的关系而引起的球体的曲率变化。该传感器通过序列检测器电路将旋转方向和转速的变化转换为一组数字记录。个人计算机自动读取并以文件的形式存储采集的数据。计算并显示用于评估的角度方向和距离和速度的接近行为。