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Analysis of neuronal circuitry in generation of insect behavior by simultaneous quantitative recordings of behavioral and neuronal activities.

通过同时定量记录行为和神经元活动来分析昆虫行为生成中的神经元回路。

基本信息

批准号：
03454017
负责人：
HISADA Mituhiko
金额：
$ 3.84万
依托单位：
Science University of Tokyo (1993)Hokkaido University (1991-1992)
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

Orientation and taxic approach by female cricket, Gryllus bimaculatus, placed atop of a spherical treadmill floated by air was recorded via infrared sensors together with its neuronal activity. Visual stimuli were given by projecting various images on the screen surrounding the cricket together with the acoustic stimuli.1. A large dark object presented in medium and far distance attracts the female as a shadow to hide during her approach to the acoustic sound source. A small horizontally oblong rectangle placed with 5 to 6 cm appears to attract the female as a male visual model. Thus, female cricket thus utilizes visual cue(s) in close encounter with her mate.2. Male courtship song has, if fully mature, a basic frequency of 5.7kHz. Five to 6 days are necessary for the full development of the courtship song after his final molt. During this period, the basic frequency becomes gradually higher starting from about 3kHz at the second day of emergence. This rise in frequency apperas to be due to the hardening of chitinous exoskeleton.3. Chitin polymerization during this period was studied by X-ray diffraction and Infrared spectroscopy. This project is yet to be completed.4. Full fledged mating behavior seems to start only after the exoskeletal chitin becomes sufficiently hardened. The courtship song of 5.7kHz basic frequency of a mature male shows stronger attraction than the song of lower basic frequencyf a young male.05. Female sensory system appears to be tuned in the frequency range of this well developed courtship song.6. Further questions remain to be studied such as how this skeletal hardening is coordinated with the behavioral repertoire, and whether the chitin polymerization or water evaporation from the wing is responsible for the frequency rise in courtship song.

通过红外传感器记录了雌性蟋蟀（Gryllus bimaculatus）的定位和趋化方法，并记录了其神经活动。视觉刺激是通过在蟋蟀周围的屏幕上投射各种图像以及声学刺激来给予的。一个巨大的黑色物体出现在中等和较远的距离吸引女性作为一个阴影隐藏在她接近声源。一个小的水平长方形放置5至6厘米似乎吸引女性作为男性的视觉模型。因此，雌性蟋蟀因此利用视觉线索与她的配偶近距离接触。雄性求偶歌的基本频率为5.7kHz。五至六天是必要的充分发展的求爱歌曲后，他的最后蜕皮。在此期间，基频从羽化第二天的约3kHz开始逐渐变高。这种频率的上升似乎是由于几丁质外泌体的硬化。用X射线衍射和红外光谱研究了这一过程中甲壳素的聚合。该项目尚未完成。完全成熟的交配行为似乎只有在外骨骼几丁质变得足够硬化后才开始。成熟雄鸟基频为5.7kHz的求偶歌比低基频的幼鸟求偶歌表现出更强的吸引力。女性感觉系统似乎在这种发达的求爱歌曲的频率范围内进行了调整。6.进一步的问题仍有待研究，如骨骼硬化是如何与行为的剧目，以及是否甲壳素聚合或水蒸发从翅膀是负责频率上升求偶歌。