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Immunological study on the mechanisms of local eosinophil infiltrations in helminthic infection.

蠕虫感染局部嗜酸性粒细胞浸润机制的免疫学研究。

基本信息

批准号：
01480169
负责人：
YOSHIMURA Kentaro
金额：
$ 4.22万
依托单位：
Akita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1991
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480169/
关键词：
Angiostrongylus cantonensis eosinophil eosinophil chemotactic factor cerebrospinal fluid monoclonal antibody hypodense eosinophil affinity chromatography chemotactic assay 間接蛍光抗体法 Mesocestoides corti ELISA Western blotting レセプタ-

项目摘要

This study deals with the possible mechanisms of local eosinophil infiltrations in Angiostrongylus cantonensis infections with special emphasis on parasite-derived eosinophil chemotactic factors and their receptors. The results obtained are sunearized as follows : Guinea-pig-eosinophils possess receptor (s) against eosinophil chemotactic factor (s) (ECF) derived from the young adult wores of A. cantonensis (YA), whereas rateosinophils are devoid of the receptor or, if any, the numbers of the receptor are few. The receptor is proteinaceous and reproducible. Hybridomas prepared from spleen cells of mice igmunized with partially Purified ECF-YA produced 2 kinds of sonoclonal antibodies (mabs) recognizing either 16.1 kD or 85 kD component in YA-whole wore extract. These mAbs were IgGl isotype. Both mAbs werespecifically reactive to ECF-YA, but failed to react to the respective whole worm extracts of A. cantonensis first stage larvae, Metastrangylus apri, Fasciola sp. and Spirometra erinace … More i plerocercoids. Several mAbs against 16.1 kD and 85 kD components all recognized the same corresponding epitopes on 16.1 kD and 85 kD antigens. Both anti-16.1 kD and anti-85 kD mAbs were capable of inhibiting eosinophil chemotactic activity of YA-whole worm extract but the former mAb gave a stronger inhibitory effect. The combination of both mAbs showed a stronger inhibitory effect than either mAb alone. Neither mAbs inhibited the chemotactic activity of ECFs derived from other helminth parasites. An affinity chromatography with anti-16.1 kD mAb succeeded in isolating a 16.1 kD antigen from YA-whole worm extracts. The ECF-YA was localized in the intestinal cytoplasm of the young adult worms. The ECF-YA can be discharged from the worms as an excretory and secretory product. Eosinophils in the cerebrospinal fluid (CSF) of mice infected with A. cantonensis were hypodense, while blood eosinophils from the same animals were normodense. Blood eosinophilia induced by the previous infection with other helminth parasite (Mesocestoides corti) failed to lead to CSF eosinophilia in subsequent A. cantonensis infection. CSF eosinophilia in A. cantonensis infection is probably provoked by ECF-YA and/or cytokines produced by lymphocytes sensitized with A. cantonensis antigens. Less

本研究探讨了广东管圆线虫感染中局部嗜酸性粒细胞浸润的可能机制，特别强调了寄生虫来源的嗜酸性趋化因子及其受体。结果表明：豚鼠嗜酸性粒细胞具有来自广东A. （YA）幼体的嗜酸性趋化因子（ECF）受体，而嗜酸性粒细胞缺乏该受体，或者即使有，受体的数量也很少。受体是蛋白质的，可再生的。用部分纯化的ECF-YA免疫小鼠脾细胞制备杂交瘤，产生2种识别ya全提取物中16.1 kD和85 kD成分的单抗。这些单克隆抗体为IgGl同型。两种单克隆抗体对ECF-YA均有特异性反应，但对广东按蚊1期幼虫全虫提取物、apri Metastrangylus apri、Fasciola sp.和Spirometra erinace均无反应。一些针对16.1 kD和85 kD成分的单克隆抗体都能识别16.1 kD和85 kD抗原上相同的对应表位。抗16.1 kD单抗和抗85 kD单抗均能抑制ya -全虫提取物的嗜酸性粒细胞趋化活性，但前者的抑制作用更强。两种单抗联合使用比单独使用任何一种单抗具有更强的抑制作用。两种单抗均不能抑制来自其他寄生虫的ecf的趋化活性。用抗16.1 kD mAb亲和层析成功地从ya全虫提取物中分离出16.1 kD抗原。ECF-YA定位于成虫的肠细胞质中。ECF-YA可以作为排泄和分泌产物从蠕虫体内排出。感染广东棘球绦虫小鼠脑脊液中嗜酸性粒细胞呈低密度，而感染广东棘球绦虫小鼠血中嗜酸性粒细胞呈正密度。先前感染其他寄生虫（中介虫）引起的血液嗜酸性粒细胞增多，在随后的广东按蚊感染中没有导致脑脊液嗜酸性粒细胞增多。广东棘球绦虫感染的脑脊液嗜酸性粒细胞增多可能是由ECF-YA和/或被广东棘球绦虫抗原致敏的淋巴细胞产生的细胞因子引起的。少