Immunological study on the mechanisms of local eosinophil infiltrations in helminthic infection.

本研究探讨广州管圆线虫感染局部嗜酸性粒细胞浸润的可能机制，特别强调寄生虫源性嗜酸性粒细胞趋化因子及其受体。结果表明：豚鼠嗜酸性粒细胞具有抗嗜酸性粒细胞趋化因子（ECF）的受体。而rateosinophil缺乏该受体，或者即使有，受体的数量也很少。该受体是蛋白质性的和可重复的。用部分纯化的ECF-YA免疫小鼠脾细胞制备杂交瘤，产生2种单克隆抗体，分别识别YA全菌提取物中16.1kD和85 kD组分。这些mAb是IgGl同种型。两种单克隆抗体对ECF-YA均呈特异性反应，但对A.广州绦虫Ⅰ期幼虫、鸡后奇线虫、片形吸虫和erinace迭宫绦虫 ...更多信息 i plerocercoids.抗16.1kD和85 kD组分的几种mAb均识别16.1kD和85 kD抗原上相同的相应表位。抗16.1kD和抗85 kD单克隆抗体均能抑制YA-全虫提取物的嗜酸性粒细胞趋化活性，但抗16.1kD单克隆抗体的抑制作用更强。两种mAb的组合显示出比单独的任一mAb更强的抑制作用。这两种单克隆抗体都不能抑制来自其他蠕虫寄生虫的ECFs的趋化活性。用抗16.1kD单克隆抗体进行亲和层析，成功地从YA-全虫提取物中分离出16.1kD抗原。ECF-YA定位于幼蠕虫的肠细胞质中。ECF-YA可以作为排泄和分泌产物从蠕虫中排出。感染A.广东血嗜酸性粒细胞低密度，而来自相同动物的血嗜酸性粒细胞正常密度。由先前感染其他蠕虫寄生虫（Mesocestoides corti）诱导的血液嗜酸性粒细胞增多症在随后的A.广州线虫感染脑脊液嗜酸性粒细胞增多症A.广州毛虫感染可能是由ECF-YA和/或A致敏淋巴细胞产生的细胞因子引起的。广州虫抗原少

This study deals with the possible mechanisms of local eosinophil infiltrations in Angiostrongylus cantonensis infections with special emphasis on parasite-derived eosinophil chemotactic factors and their receptors. The results obtained are sunearized as follows : Guinea-pig-eosinophils possess receptor (s) against eosinophil chemotactic factor (s) (ECF) derived from the young adult wores of A. cantonensis (YA), whereas rateosinophils are devoid of the receptor or, if any, the numbers of the receptor are few. The receptor is proteinaceous and reproducible. Hybridomas prepared from spleen cells of mice igmunized with partially Purified ECF-YA produced 2 kinds of sonoclonal antibodies (mabs) recognizing either 16.1 kD or 85 kD component in YA-whole wore extract. These mAbs were IgGl isotype. Both mAbs werespecifically reactive to ECF-YA, but failed to react to the respective whole worm extracts of A. cantonensis first stage larvae, Metastrangylus apri, Fasciola sp. and Spirometra erinace … More i plerocercoids. Several mAbs against 16.1 kD and 85 kD components all recognized the same corresponding epitopes on 16.1 kD and 85 kD antigens. Both anti-16.1 kD and anti-85 kD mAbs were capable of inhibiting eosinophil chemotactic activity of YA-whole worm extract but the former mAb gave a stronger inhibitory effect. The combination of both mAbs showed a stronger inhibitory effect than either mAb alone. Neither mAbs inhibited the chemotactic activity of ECFs derived from other helminth parasites. An affinity chromatography with anti-16.1 kD mAb succeeded in isolating a 16.1 kD antigen from YA-whole worm extracts. The ECF-YA was localized in the intestinal cytoplasm of the young adult worms. The ECF-YA can be discharged from the worms as an excretory and secretory product. Eosinophils in the cerebrospinal fluid (CSF) of mice infected with A. cantonensis were hypodense, while blood eosinophils from the same animals were normodense. Blood eosinophilia induced by the previous infection with other helminth parasite (Mesocestoides corti) failed to lead to CSF eosinophilia in subsequent A. cantonensis infection. CSF eosinophilia in A. cantonensis infection is probably provoked by ECF-YA and/or cytokines produced by lymphocytes sensitized with A. cantonensis antigens. Less