Molecular immunological study on the susceptibility and morbidity of T cell-chimeric mice to Angiostrongylus cantonensis

得到的结果如下：1。对B10同源小鼠的研究表明，小鼠对广州管圆线虫感染的发病率和易感性与H-2复合物无关，而与非H-2背景基因有关，抗原特异性抗体反应与小鼠对广州管圆线虫感染的易感性和发病率之间没有显著关系。2. 一项使用INF-γ敲除小鼠的研究表明，INF-γ本身不影响小鼠感染广东单胞菌的结果。3. C.B-17系小鼠嗜酸性细胞生成功能有缺陷，因此不能杀死广州蛔虫颅内蠕虫。这可能与C.B-17株骨髓细胞中β链mRNA的表达受损有关。4. 用C57BL/6与cb -17小鼠的骨髓嵌合小鼠进行的研究表明，骨髓来源的细胞主要参与广东a.b -17感染小鼠的发病表达，而非骨髓来源的细胞也参与更多细胞类型的表达。5. 将经亚致死剂量X射线照射的BALB/c-nu/nu小鼠与同源骨髓细胞重组，再与来源于C57BL/6或BALB/c的胸腺细胞移植，制备T细胞嵌合小鼠。大多数BALB/c-T细胞嵌合小鼠在感染广东单胞菌前死亡，感染BALB/c-T细胞嵌合小鼠在感染后也很快死亡。与nu/nu小鼠相比，假嵌合小鼠显示出蠕虫发育受损，并且在感染广东a.c anensis后也表现出明显的体重减轻和高死亡率，尽管它们产生的蠕虫负担与nu/nu或+/+小鼠相似。这些数据表明，BALB/c的T细胞与感染后的发病率有关，而C57BL/6的T细胞有助于改善发病率。6. 检测cb -17和C57BL/6小鼠F1、F2和回交后代的易感性、嗜酸性粒细胞反应和发病率。这些反应被发现由多个基因控制，并且在蠕虫恢复与脑脊液中嗜酸性粒细胞水平之间存在弱正相关。7. 感染BALB/c小鼠的脾CD4^+T细胞对几种22 ~ 56 kD的蛋白抗原表现出增殖反应。这表明CD4^+T细胞的这些反应是导致发病的原因。少

The results obtained are as follows : 1. A study using B10 congenic mice indicated that murine morbidity and susceptibility to Angiostrongylus cantonensis infection are not linked to the H-2 complex but rather to non-H-2 background genes and also that there is no significant relationship between antigen-specific antibody responses and murine susceptibility or morbidity to A.cantonensis infection. 2. A study using INF-γ knockout mice suggested that INF-γ itself did not affect the outcome of A.cantonensis infection in mice. 3. C.B-17 strain mice have the defective eosinopoiesis and are thereby incapable of killing intracranial worms of A.cantonensis. This is probably due to the impaired expression of β-chain mRNA in bone marrow cells of C.B-17 strain. 4. A study using bone marrow chimeric mice between C.B-17 and C57BL/6 mice suggests that the bone marrow derived cells are primarily involved in the expression of morbidity in A.cantonensis infected C.B-17 mice but non-bone marrow derived c … More ell types are also concerned partly. 5. T cell-chimeric mice were produced by the procedure that BALB/c-nu/nu mice which had been irradiated with sublethal dose of X ray and additionally treated with anti-Thy-1.2 antibody were reconstituted with homologous bone marrow cells and then transferred with thymus cells derived from C57BL/6 or BALB/c. A majority of BALB/c-T cell-chimeric mice died before A.cantonensis infection and the infected BALB/c-T cell-chimeric mice also died soon after infection. Sham chimeric mice showed the impaired worm development when compared with nu/nu mice, and also exhibited marked body weight loss and high mortality after A.cantonensis infection although they yielded a similar level of worm burdens as nu/nu or +/+ mice. These data suggest that T cells of BALB/c are associated with morbidity after infection whereas T cells from C57BL/6 contribute to improving morbidity. 6. F1, F2 and back-cross progenies between C.B-17 and C57BL/6 mice were examined for susceptibility, eosinophil responses and morbidity. These responses were found to be controlled by multiple genes, and a weak positive correlation was recognized between worm recovery and the level of eosinophils in cerebrospinal fluid. 7. Splenic CD4^+T cells from infected BALB/c mice showed a proliferative response against several protein antigens ranging from 22 to 56 kD.This suggests that these responses by CD4^+T cells are responsible for inducing morbidity. Less