Development ofrapid cancer diagnosis by the novel electrospray ionization-mass spectrometry

新型电喷雾电离质谱法快速癌症诊断的发展

• 批准号: 23790619
• 负责人: YOSHIMURA Kentaro
• 金额: $ 2.75万
• 依托单位: University of Yamanashi
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23790619/
• 关键词: 質量分析; 学習機械; がん診断; 大気圧; エレクトロスプレー; ベイズ統計学

Probe electrospray ionization (PESI) is a biocompatible ambient ionization technique. We determined the conditions of measurements for lipids in human tissues. More than 3,000 spectra datasets were obtained from specimens of human renal cell carcinoma and hepatocellular carcinoma. These data were used as a training dataset, which serves as a reference for the dPLRM. We input randomly selected test datasets to the dPLRM to get probabilistic result of cancer diagnosis. We achieved over 90% coincidence of diagnosis with those judged by the conventional histopathology.

探针电喷雾电离（PESI）是一种生物相容性的环境电离技术。我们确定了人体组织中脂质的测量条件。从人肾细胞癌和肝细胞癌的标本中获得了3,000多个光谱数据集。这些数据被用作培训数据集，该数据集用作DPLRM的参考。我们将随机选择的测试数据集输入DPLRM，以获得癌症诊断的概率结果。我们与常规组织病理学判断的诊断相结合了90％以上。

项目成果

Direct Analysis of Biomolecules by Probe Electrospray Ionization Mass Spectrometry

通过探针电喷雾电离质谱法直接分析生物分子

• 发表时间: 2011
• 作者: Yoshimura K.;Chen L.C.;Zhan Y.;Hiraoka K.;Takeda S;Mridul Kanti Mandal;Mridul Kanti Mandal
• 通讯作者: Mridul Kanti Mandal

Characterization of ciliary targeting sequence of rat melanin-concentrating hormone receptor 1

• DOI: 10.1016/j.ygcen.2013.02.020
• 发表时间: 2013-07-01
• 期刊: GENERAL AND COMPARATIVE ENDOCRINOLOGY
• 影响因子: 2.7
• 作者: Nagata, Asami;Hamamoto, Akie;Saito, Yumiko
• 通讯作者: Saito, Yumiko

Hedgehog signaling regulates the early stage of myelination by Schwann cells through the primary cilia

Hedgehog 信号通过初级纤毛调节雪旺细胞髓鞘形成的早期阶段

• 发表时间: 2011
• 作者: Yoshimura K.;Takeda S.
• 通讯作者: Takeda S.

Rapid cancer diagnosis using electrospray ionization mass spectrometry

使用电喷雾电离质谱法快速诊断癌症

• 发表时间: 2011
• 作者: Yoshimura K.;Chen L.C.;Mandal M.K.;Hiraoka K.;Takeda S.
• 通讯作者: Takeda S.

Hedgehog signaling facilitates the myelination by Schwann cells through the primary cilia

Hedgehog 信号传导通过初级纤毛促进雪旺细胞的髓鞘形成

• 发表时间: 2011
• 作者: Yoshimura K.;Takeda S
• 通讯作者: Takeda S