Immunological studies on cerebrospinal fluid eosinophilia and the possible roles of eosinophils in angiostrongyliasis cantonensis

脑脊液嗜酸性粒细胞增多的免疫学研究及其在广州管圆线虫病中的可能作用

• 批准号: 62570170
• 负责人: YOSHIMURA Kentaro
• 金额: $ 1.47万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570170/
• 关键词: Angiostrongylus cantonensis; eosinophil; cerebrospinal fluid; bone marrow culture; eosinophil chemotactic factor of parasite; receptor; superoxide anion; 脱髄; マウス; スーパーオキサイド

The results obtained are summarized as follows: 1. Systemic eosinophilia; (1) Lymphoid cells were harvested from the spleen and cervical lymph-nodes of AF-nu/+ mice infected with Angiostrongylus cantonensis for 12 to 18 days. Three separate intravenous injections of 5 X 10^7 cells (obtained at days 12, 15 and 18) were given to recipient AF-nu/nu mice. These mice provoked significant peripheral eosinophilia from days 6 to 15 after final injection. (2) Mediastinal lymph-node cells were harvested from Wistar rats (permissive host) at 30 days postinfection, and stimulated with the antigen of 1st stage larvae. A conditioned medium thus prepared was found to be effective for in vitro survival of medullary eosinophils. (3) The splenic and cervical lymph-node cells were obtained from ddY mice (nonpermissive host) at 5, 12 and 17 days postinfection, and cultured with young adult worm antigen for harvesting a conditioned medium. This conditioned medium was highly effective in in vitro eosinopoie … More sis or survival of eosinophils. The lymphoid cells from infected mice can also release such a factor(s) into media without antigenic stimulation.2. Local eosinophilia; (1) An eosinophil chemotactic factor (ECF) derived from the young adult worms of A. cantonensis was resistant to both heating and pronase digestion, but sensitive to periodate treatment, with an approximate molecular weight of 16900. (2) Guinea-pig eosinophils possess receptors for ECFs of both young adult worms and 1st-stage larvae, whereas rat eosinophils have receptors for ECF of only Lst-stage larvae. (3) Eosinophil receptors for ECF of young adult worms were destroyed by pronase digestion but resynthesized during 24 hrs of cell cultivation.3. Roles of cerebrospinal fluid (CSF) eosinophils; (1) Degranulative changes were noted in CSF eosinophils from infected guinea-pigs. (2) CSF eosinophils stimulated by young adult worm antigen generated O_2 ・ These findings are suggestive of the possible association of eosinophils with the killing of intracranial worms in nonpermissive hosts. (3) Demyelinative changes and the partial loss of Purkinje cells were noted in the cerebellar tissues of infected mice, suggesting possible involvement of CSF eosinophils in the damages of central nervous system.4. Possible roles of eosinophils in the protective immunity in permissive rat host; Rats (ACI, AUG and Wistar) received a sensitizing infection developed significant protective immunity against challenge infection at 6 weeks later. Young abult worms transferred into the peritoneal cavity of these sensitized rats were found infiltrated predominantly with eosinophils and most of the eosinophils were degranulative. Less

主要研究结果如下：1.全身性嗜酸性粒细胞增多症;（1）从感染广州管圆线虫12 ~ 18天的AF-nu/+小鼠的脾和颈淋巴结中分离类淋巴细胞。将5 × 10^7个细胞（在第12、15和18天获得）三次单独静脉注射给受体AF-nu/nu小鼠。这些小鼠在末次注射后第6 - 15天引起显著的外周嗜酸性粒细胞增多。(2)在感染后30天，从Wistar大鼠（许可宿主）收获纵隔淋巴结细胞，并用第1期幼虫抗原刺激。由此制备的条件培养基被认为是有效的髓嗜酸性粒细胞在体外生存。(3)在感染后5、12和17天，从ddY小鼠（非允许宿主）获得脾和颈淋巴结细胞，并与年轻成虫抗原一起培养以收获条件培养基。该条件培养基在体外培养中对嗜酸性粒细胞的生成具有高效作用 ...更多信息 或嗜酸性粒细胞存活。来自感染小鼠的淋巴样细胞也可以在没有抗原刺激的情况下将这样的因子释放到培养基中。局部嗜酸性粒细胞：（1）嗜酸性粒细胞趋化因子（ECF）来源于A. cantonensis对加热和链霉蛋白酶消化都有抗性，但对高碘酸盐处理敏感，分子量约为16900。(2)豚鼠嗜酸性粒细胞具有年轻蠕虫和第1阶段的幼虫的ECF受体，而大鼠嗜酸性粒细胞只有LST-阶段的幼虫的ECF受体。(3)幼蠕虫ECF的嗜酸性粒细胞受体被链霉蛋白酶消化破坏，但在24小时的细胞培养过程中重新合成。脑脊液（CSF）嗜酸性粒细胞的作用：（1）感染豚鼠的CSF嗜酸性粒细胞出现脱颗粒改变。(2)脑脊液嗜酸性粒细胞在幼成虫抗原刺激下产生O_2。这些结果提示嗜酸性粒细胞可能与杀死非许可宿主颅内蠕虫有关。(3)感染后小鼠小脑组织出现脱髓鞘改变和部分浦肯野细胞丢失，提示脑脊液嗜酸性粒细胞可能参与了中枢神经系统的损害.嗜酸性粒细胞在宿主保护性免疫中的可能作用：大鼠（ACI，AUG和Wistar）接受致敏感染后，在6周后对攻击感染产生了显著的保护性免疫。转移到致敏大鼠腹腔的幼蠕虫主要由嗜酸性粒细胞浸润，且大多数嗜酸性粒细胞脱颗粒。少

项目成果

Yoshimura,K.;Sugaya,H.;Kawamura,K.;Kumagai,M.: Parasite Immunology. 10. 411-424 (1988)

Yoshimura,K.；Sugaya,H.；Kawamura,K.；Kumagai,M.：寄生虫免疫学。

菅谷博子,吉村堅太郎,川村公一,石田和人,熊谷正広,石郷岡清基,山下恵子: 寄生虫学雑誌. 36. 34 (1987)

Hiroko Sugaya、Kentaro Yoshimura、Koichi Kawamura、Kazuto Ishida、Masahiro Kumagai、Kiyoshiki Ishigooka、Keiko Yamashita：寄生虫学杂志 36. 34 (1987)。

Yoshimura, K.; Ishida, K.; Ishigooka, S.; Sugaya, H.; Kumagai, M.: "Protective Immunity against Angiostrongylus cantonensis in rats following sensitizing infections" Parasitology Research.

吉村，K.；

Yoshimura, K., Sugaya, H., Kawamura, K. & Kumagai, M.: Parasite Immunology. 10. (1988)

吉村，K.，菅谷，H.，川村，K.

Sugaya,H.;Yoshimura,K.: Parasite immunology. 10. (1988)

Sugaya，H.；Yoshimura，K.：寄生虫免疫学。