The mechanism of eosinophil accumulation in the cerebrospinal fluid of Angiostrongylus cantonensis infected hosts

所得结果总结如下：1。非允许宿主（小鼠）嗜酸性粒细胞的积累：从感染广东管圆线虫（A.c） 20天的AF杂合（nu/+）小鼠的脑脊液（CSF）中收集嗜酸性粒细胞，或从感染corti (M.c) 20天的nu/+小鼠的腹腔中收集嗜酸性粒细胞，iv转移到未感染的正常nu/nu小鼠或感染A.c 17天的小鼠中。只有嗜酸性粒细胞转移的a.c感染的nu/nu小鼠脑脊液中出现了嗜酸性粒细胞的积累。a.c感染的脑脊液嗜酸性粒细胞增多的nu/nu小鼠颅内蠕虫恢复明显低。将a.c感染的nu/+小鼠脾脏细胞与脑脊液嗜酸性粒细胞联合注射，可抑制a.c感染的nu/nu小鼠脑脊液中嗜酸性粒细胞的积累。从脑脊液或腹腔中转移荧光标记的嗜酸性粒细胞表明，受体nu/nu小鼠脑脊液中积累的几乎所有嗜酸性粒细胞本身都被转移。这些数据表明，颅内蠕虫在受体小鼠体内的预先存在是脑脊液嗜酸性粒细胞积累的先决条件，并且脑脊液嗜酸性粒细胞参与了颅内蠕虫的杀伤。2. 在容许宿主（大鼠）中嗜酸性粒细胞积累：对(1)A.c感染40-43天，(2)M.c感染20-23天，(3)A.c成虫感染22-23天的外周嗜酸性粒细胞增多的大鼠进行脑室内注射A.c-幼成虫（YA）抗原，A.c卵抗原或M.c抗原。对各组大鼠脑脊液嗜酸性粒细胞的监测表明，注射a.c卵或M.c抗原后，a.c感染大鼠的脑脊液嗜酸性粒细胞出现积累，而不注射YA抗原。这些发现与a.c卵和M.c抗原对大鼠嗜酸性粒细胞的体外趋化活性一致。似乎有可能是嗜酸性粒细胞的诱导

Results obtained are summatrized as follows : 1. Eosinophil accumulation in nonpermissive host (mice) : Cerebrospinal fluid (CSF) eosinophils collected from AF heterozygous (nu/+) mice infected with Angiostrongylus cantonensis (A.c) for 20 days or peritoneal eosinophils harvested from the peritoneal cavity of nu/+ mice infected with Mesocestoides corti (M.c) for 20 days were iv rtansferred into uninfected, normal nu/nu mice or into those infected with A.c for 17 days. Eosinophil accumulation in CSF occurred in only A.c-infected nu/nu mice transferred with eosinophils. A.c-infected nu/nu mice with CSF eosinophilia showed significantly low intracranial worm recovery. A combined injection of spleen cells from A.c-infected nu/+ mice along with CSF eosinophils caused the suppression of eosinophil accumulation in the CSF of A.c-infected nu/nu mice. Transfers of fluorochrome-labeled eosinophils from CSF or peritoneal cavity indicated that almost all eosinophils accumulated in the CSF of the recipient nu/nu mice were transferred-eosinophils themselves. These data suggest that the pre-existence of intracranial worms in the recipient mice is a prersquisite for CSF eosinophil accumulation and also that CSF eosinophilia is involved in the killing of intracranial worms. 2. Eosinophil accumulation in permissive host (rats) : Intraventricular injection of A.c-young adult worm (YA) antigen, A.c-egg antigen, or M.c antigen was performed in rats with peripheral eosinophilia due to (1)A.c infection for 40-43 days, (2) M.c infection for 20-23 days and (3) A.c-adult worm infection for 22-23 days following intrapulmonary transfers of YA.Monitoring of CSF eosinophilia in these rat groups indicated that CSF eosinophil accumulation occurred in A.c-infected rats following injection with A.c-egg or M.c antigen but not with YA antigen. These findings coincide with in vitro chemotactic activity of A.c-egg and M.c antigens against rat-eosinophils. It seems likely that the induction of eosinophi