The mechanism of eosinophil accumulation in the cerebrospinal fluid of Angiostrongylus cantonensis infected hosts

1.不允许宿主(小鼠)的嗜酸性粒细胞聚集：将感染广州管圆线虫(A.C)20天的AF杂合子(NU/)小鼠的脑脊液(CSF)中的嗜酸性粒细胞或感染介体线虫(M.C)20天的NU/NU/小鼠的腹膜嗜酸性粒细胞静脉注射到未感染的正常NU/NU/NU小鼠或感染A.C感染17天的NU/NU/NU小鼠体内。仅在感染AC的nu/nu小鼠转移了嗜酸性粒细胞后，脑脊液中才有嗜酸性粒细胞聚集。伴有脑脊液嗜酸粒细胞增多症的AC感染的nu/nu小鼠表现出显著的低颅内蠕虫回收率。联合注射来自AC感染的nu/nu小鼠的脾细胞和脑脊液中的嗜酸性粒细胞，可抑制AC感染的nu/nu小鼠脑脊液中的嗜酸性粒细胞聚集。从脑脊液或腹腔转移荧光标记的嗜酸性粒细胞表明，几乎所有聚集在受体nu/nu小鼠脑脊液中的嗜酸性粒细胞都是转移的嗜酸性粒细胞。这些数据表明，在受体小鼠中，颅内蠕虫的预先存在是脑脊液嗜酸性粒细胞聚集的先决条件，而且脑脊液嗜酸性粒细胞参与了杀死颅内蠕虫的过程。2.嗜酸性粒细胞在允许宿主(大鼠)中的聚集：将A.C-幼虫(YA)抗原、A.C-虫卵抗原或M.C抗原注射到(1)A.C感染所致的外周嗜酸性粒细胞增多症大鼠的脑室，持续40-43天，(2)肺炎支原体感染20-23天；(3)感染A.C-成虫22-23天。对各组大鼠脑脊液嗜酸性粒细胞的监测表明，注射A.C-虫卵或M.C抗原后，感染A.C的大鼠脑脊液中可见嗜酸性粒细胞聚集，但注射YA抗原后未见。这些发现与A.C-鸡蛋和M.C抗原对大鼠嗜酸性粒细胞的体外趋化活性相一致。看来，嗜酸性粒细胞的诱导

Results obtained are summatrized as follows : 1. Eosinophil accumulation in nonpermissive host (mice) : Cerebrospinal fluid (CSF) eosinophils collected from AF heterozygous (nu/+) mice infected with Angiostrongylus cantonensis (A.c) for 20 days or peritoneal eosinophils harvested from the peritoneal cavity of nu/+ mice infected with Mesocestoides corti (M.c) for 20 days were iv rtansferred into uninfected, normal nu/nu mice or into those infected with A.c for 17 days. Eosinophil accumulation in CSF occurred in only A.c-infected nu/nu mice transferred with eosinophils. A.c-infected nu/nu mice with CSF eosinophilia showed significantly low intracranial worm recovery. A combined injection of spleen cells from A.c-infected nu/+ mice along with CSF eosinophils caused the suppression of eosinophil accumulation in the CSF of A.c-infected nu/nu mice. Transfers of fluorochrome-labeled eosinophils from CSF or peritoneal cavity indicated that almost all eosinophils accumulated in the CSF of the recipient nu/nu mice were transferred-eosinophils themselves. These data suggest that the pre-existence of intracranial worms in the recipient mice is a prersquisite for CSF eosinophil accumulation and also that CSF eosinophilia is involved in the killing of intracranial worms. 2. Eosinophil accumulation in permissive host (rats) : Intraventricular injection of A.c-young adult worm (YA) antigen, A.c-egg antigen, or M.c antigen was performed in rats with peripheral eosinophilia due to (1)A.c infection for 40-43 days, (2) M.c infection for 20-23 days and (3) A.c-adult worm infection for 22-23 days following intrapulmonary transfers of YA.Monitoring of CSF eosinophilia in these rat groups indicated that CSF eosinophil accumulation occurred in A.c-infected rats following injection with A.c-egg or M.c antigen but not with YA antigen. These findings coincide with in vitro chemotactic activity of A.c-egg and M.c antigens against rat-eosinophils. It seems likely that the induction of eosinophi