The mechanism of eosinophil accumulation in the cerebrospinal fluid of Angiostrongylus cantonensis infected hosts

广州管圆线虫感染宿主脑脊液中嗜酸性粒细胞积聚的机制

• 批准号: 04670223
• 负责人: YOSHIMURA Kentaro
• 金额: $ 1.34万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1992
• 资助国家: 日本
• 起止时间: 1992 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-04670223/
• 关键词: Angiostrongylus cantonensis; Mesocestoides corti; eosinophils; cerebrospinal fluid; nude mice; blood-brain barrier; eosinophil chemotactic factor; 好酸球遊走因子

Results obtained are summatrized as follows : 1. Eosinophil accumulation in nonpermissive host (mice) : Cerebrospinal fluid (CSF) eosinophils collected from AF heterozygous (nu/+) mice infected with Angiostrongylus cantonensis (A.c) for 20 days or peritoneal eosinophils harvested from the peritoneal cavity of nu/+ mice infected with Mesocestoides corti (M.c) for 20 days were iv rtansferred into uninfected, normal nu/nu mice or into those infected with A.c for 17 days. Eosinophil accumulation in CSF occurred in only A.c-infected nu/nu mice transferred with eosinophils. A.c-infected nu/nu mice with CSF eosinophilia showed significantly low intracranial worm recovery. A combined injection of spleen cells from A.c-infected nu/+ mice along with CSF eosinophils caused the suppression of eosinophil accumulation in the CSF of A.c-infected nu/nu mice. Transfers of fluorochrome-labeled eosinophils from CSF or peritoneal cavity indicated that almost all eosinophils accumulated in the CSF of the recipient nu/nu mice were transferred-eosinophils themselves. These data suggest that the pre-existence of intracranial worms in the recipient mice is a prersquisite for CSF eosinophil accumulation and also that CSF eosinophilia is involved in the killing of intracranial worms. 2. Eosinophil accumulation in permissive host (rats) : Intraventricular injection of A.c-young adult worm (YA) antigen, A.c-egg antigen, or M.c antigen was performed in rats with peripheral eosinophilia due to (1)A.c infection for 40-43 days, (2) M.c infection for 20-23 days and (3) A.c-adult worm infection for 22-23 days following intrapulmonary transfers of YA.Monitoring of CSF eosinophilia in these rat groups indicated that CSF eosinophil accumulation occurred in A.c-infected rats following injection with A.c-egg or M.c antigen but not with YA antigen. These findings coincide with in vitro chemotactic activity of A.c-egg and M.c antigens against rat-eosinophils. It seems likely that the induction of eosinophi

所得结果如下：1.非许可宿主（小鼠）中嗜酸性粒细胞蓄积：从感染广州管圆线虫的AF杂合子（nu/+）小鼠收集的脑脊液（CSF）嗜酸性粒细胞（A. c）20天，或从感染科氏中囊线虫的nu/+小鼠的腹膜腔收获的腹膜嗜酸性粒细胞（M. c）感染20天的小鼠静脉转移到未感染的正常nu/nu小鼠或A. c感染17天的小鼠中。CSF中嗜酸性粒细胞蓄积仅发生在嗜酸性粒细胞转移的A. c感染nu/nu小鼠中。具有CSF嗜酸性粒细胞增多的A. c感染的nu/nu小鼠显示出显著低的颅内蠕虫恢复。将A. c感染的nu/+小鼠的脾细胞与CSF嗜酸性粒细胞联合注射沿着，可抑制A. c感染的nu/nu小鼠CSF中的嗜酸性粒细胞蓄积。荧光染料标记的嗜酸性粒细胞从CSF或腹膜腔的转移表明，几乎所有的嗜酸性粒细胞聚集在受体nu/nu小鼠的CSF中的转移嗜酸性粒细胞本身。这些数据表明，颅内蠕虫在受体小鼠中的预先存在是CSF嗜酸性粒细胞积聚的先决条件，并且CSF嗜酸性粒细胞参与颅内蠕虫的杀死。2.允许宿主（大鼠）中嗜酸性粒细胞蓄积：在患有外周嗜酸性粒细胞增多症的大鼠中进行A. c-青年成虫（YA）抗原、A. c-卵抗原或M. c抗原的脑室内注射，所述外周嗜酸性粒细胞增多症是由于（1）A.c感染40-43天，（2）M. c感染20-23天;（3）A. c-成虫感染22- 24天。肺内转移YA后23天。对这些大鼠组中CSF嗜酸性粒细胞的监测表明，在注射A.c-后，A. c感染的大鼠中发生了CSF嗜酸性粒细胞积聚卵或M. c抗原，但不与YA抗原。这些发现与A. c-卵和M. c抗原对大鼠嗜酸性粒细胞的体外趋化活性一致。似乎嗜酸性粒细胞的诱导

项目成果

Yoshimura, K.& Sugaya, H.: "The mechanism of eosinophil accumulation in the cerebrospinal fulid of mice infected with Angiostrongylus cantonensis."

吉村，K.

Ishida, K & Yoshimura, K.: "Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis" Parasite Immunol.14. 633-644 (1992)

石田，K

Yoshimura, K.: "The mechanism of parasite killing by eosinophils in parasitic infections(in Japanese with English abstract)" Jpn.J.Clin.Med.51. 657-663 (1993)

Yoshimura, K.：“寄生虫感染中嗜酸性粒细胞杀死寄生虫的机制（日文，英文摘要）”Jpn.J.Clin.Med.51。

Ishida,Kazuto: "Characterization of monoclonal antibodies against eosinophil chemotactic factors from young adult worms of Angiostrongylus cantonensis." Parasite Immunology. 14. 633-644 (1992)

Ishida，Kazuto：“针对来自广州管圆线虫幼年成虫的嗜酸性粒细胞趋化因子的单克隆抗体的表征。”

Yoshimura, K., Sugaya, H., Ishida, K., Khan, W.I., Abe, T.& Uno, K.: "The effect of cyclosporin A on Angiostrongylus cantonensis infection and eosinophilia in mice" Int.J.Parasitol.23. 997-1003 (1993)

吉村，K.，菅谷，H.，石田，K.，可汗，W.I.，阿部，T.