The mechanism of eosinophil accumulation in the cerebrospinal fluid of Angiostrongylus cantonensis infected hosts

所得结果如下：1.非许可宿主（小鼠）中嗜酸性粒细胞蓄积：从感染广州管圆线虫的AF杂合子（nu/+）小鼠收集的脑脊液（CSF）嗜酸性粒细胞（A.c）20天或从感染科尔蒂中棘虫的nu/+小鼠的腹腔中收获腹膜嗜酸性粒细胞（M. c）感染20天的小鼠静脉转移到未感染的正常nu/nu小鼠或A. c感染17天的小鼠中。CSF中嗜酸性粒细胞蓄积仅发生在嗜酸性粒细胞转移的A. c感染nu/nu小鼠中。具有CSF嗜酸性粒细胞增多的A. c感染的nu/nu小鼠显示出显著低的颅内蠕虫恢复。将A. c感染的nu/+小鼠的脾细胞与CSF嗜酸性粒细胞联合注射沿着，可抑制A. c感染的nu/nu小鼠CSF中的嗜酸性粒细胞蓄积。荧光染料标记的嗜酸性粒细胞从CSF或腹膜腔的转移表明，几乎所有的嗜酸性粒细胞聚集在受体nu/nu小鼠的CSF中的转移嗜酸性粒细胞本身。这些数据表明，颅内蠕虫在受体小鼠中的预先存在是CSF嗜酸性粒细胞积聚的先决条件，并且CSF嗜酸性粒细胞参与颅内蠕虫的杀死。2.允许宿主（大鼠）中嗜酸性粒细胞蓄积：在患有外周嗜酸性粒细胞增多症的大鼠中进行A. c-青年成虫（YA）抗原、A. c-卵抗原或M. c抗原的脑室内注射，所述外周嗜酸性粒细胞增多症是由于（1）A.c感染40-43天，（2）M. c感染20-23天;（3）A. c-成虫感染22- 24天。在这些大鼠组中监测CSF嗜酸性粒细胞增多表明，在用A.c.注射后，在A.c.感染的大鼠中发生CSF嗜酸性粒细胞积聚。卵或M. c抗原，但不与YA抗原。这些发现与A. c-卵和M. c抗原对大鼠嗜酸性粒细胞的体外趋化活性一致。似乎嗜酸性粒细胞的诱导

Results obtained are summatrized as follows : 1. Eosinophil accumulation in nonpermissive host (mice) : Cerebrospinal fluid (CSF) eosinophils collected from AF heterozygous (nu/+) mice infected with Angiostrongylus cantonensis (A.c) for 20 days or peritoneal eosinophils harvested from the peritoneal cavity of nu/+ mice infected with Mesocestoides corti (M.c) for 20 days were iv rtansferred into uninfected, normal nu/nu mice or into those infected with A.c for 17 days. Eosinophil accumulation in CSF occurred in only A.c-infected nu/nu mice transferred with eosinophils. A.c-infected nu/nu mice with CSF eosinophilia showed significantly low intracranial worm recovery. A combined injection of spleen cells from A.c-infected nu/+ mice along with CSF eosinophils caused the suppression of eosinophil accumulation in the CSF of A.c-infected nu/nu mice. Transfers of fluorochrome-labeled eosinophils from CSF or peritoneal cavity indicated that almost all eosinophils accumulated in the CSF of the recipient nu/nu mice were transferred-eosinophils themselves. These data suggest that the pre-existence of intracranial worms in the recipient mice is a prersquisite for CSF eosinophil accumulation and also that CSF eosinophilia is involved in the killing of intracranial worms. 2. Eosinophil accumulation in permissive host (rats) : Intraventricular injection of A.c-young adult worm (YA) antigen, A.c-egg antigen, or M.c antigen was performed in rats with peripheral eosinophilia due to (1)A.c infection for 40-43 days, (2) M.c infection for 20-23 days and (3) A.c-adult worm infection for 22-23 days following intrapulmonary transfers of YA.Monitoring of CSF eosinophilia in these rat groups indicated that CSF eosinophil accumulation occurred in A.c-infected rats following injection with A.c-egg or M.c antigen but not with YA antigen. These findings coincide with in vitro chemotactic activity of A.c-egg and M.c antigens against rat-eosinophils. It seems likely that the induction of eosinophi