Histo pathological and clinical application of placental alkaline phostaphase as a tumor maker for primary intracranial germinoma.

胎盘碱性磷酸化作为原发性颅内生殖细胞瘤标记物的组织病理学和临床应用。

• 批准号: 61570694
• 负责人: HIRATA Toshifumi
• 金额: $ 0.96万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570694/
• 关键词: placental alkaline phosphatase (PLAP); germinoma; immunohistochemistory; enzyme-linked immunoabsorbent assay(ESISA); ELISA(enzyme-linked immunoabsorbent assay); 胚腫(germinoma); ELISA(enzyme-linked immunoaborbent assay); 胎盤アルカリフオスファターゼ(PLAP); 胎盤アルカリ

1. Indirect immunoperoxidase ataining was carried out on 23 primary intracranial germ-cell tumors (17 germinomas, 1 embryonal carcinoma, 1 yolk sac tumor, 3 teratomas, and 1 teratoma with embryonal carcinoma) and on 6 primary pineal non-germ-cell tumors (1 pineocytoma, 2 pineoblastomas, 2 astrocytomas, and 1 glioblastoma). Thirteen of 17 intracranial germinomas (76.5%) showed positive staining for placental alkaline phosphatase (PLAP) mainly on the tumor cell membrane. In 6 primary intractanial non-germinomatous germ-cell tumors, there was weak positive staining indicating the presence of PLAP only a few cells of one embryonal carcinoma, and in some glandular epithelial cells of one teratoma; This staining was limited to the cytoplasm. None of the other 6 pineal non-germ-cell tumors showed any positive PLAP reaction.2. Eighty-five (29 intracranial and 56 testicular) germ-cell tumors were studied immunohistochemically. Germinoma (seminoma) component was seen in 52 cases. Forty-eight of … More these cases (92.3%) contained many PLAP-positive tumor cells. Teratoma component was seen in 20 cases. In the 20 cases, 5 (25.0%) were found to have some PLAP-positive glandular epithelial cells. Embryonal carcinoma component was seen in 14 cases. Four of these cases (28.6%) contained some of PLAP-positive tumor cells. Yolk sac tumor component was seen 12 cases. Only one of the cases (8.3%) had several tumor cells showing a positive reaction for PLAP. Choriocarcinoma component was seen in 4 cases. In the 4 cases, 3(75.0%) were found to have a small number of syncytiotrophoblastic tumor cells showing a positive PLAP reaction.3. A sensitive enzyme-limked immunoabsorbent assay (ELISA) for assessing PLAP was established and used in an examination of PLAP measurement in varous brain tumorpatiens. The PLAP levels in serum of 47 control adult male individuals and in the CSF of 20 non-prehnant patients of subarachnoid hemorrhage were measured. The upper limits of the above samples were 0.29 and 0.11 IU/L in the serum and the CSF, respectively. All of the PLAP levels in sera of 68 patients with various brain tumors expect germinoma (2 teratomas, 1 yolk sac tumor with immture teratoma, 1 metastatic choriocarcinoma from the testis, 14 glioblastomas, 18 astrocytomas, 19 meningiomas, and 13 neurinomas) were 0.29 IU/L or less. Five of 6 germinoma patients had elevated PLAP levels in their serum (0.52 to 3.78 IU/L) and 4 of 5 also had elevated LPAP levels in their CSF (0.83 to 9.83 IU/L). PLAP levels in the intratumoral cyst fluid of 2 germinomas were very high (603 and 863 IU/L).4. These studies strongly suggests that PLAP is a useful tumor marker for primary in intracranial germinoma not only histopathologically but also clinically. Less

1.对23例原发性颅内生殖细胞肿瘤（17例生殖细胞瘤、1例胚胎癌、1例卵黄囊肿瘤、3例畸胎瘤、1例畸胎瘤伴胚胎癌）和6例原发性松果体非生殖细胞肿瘤（1例松果体细胞瘤、2例松果体母细胞瘤、2例星形细胞瘤、 和 1 例胶质母细胞瘤）。 17 例颅内生殖细胞瘤中有 13 例 (76.5%) 主要在肿瘤细胞膜上显示胎盘碱性磷酸酶 (PLAP) 阳性染色。在6例原发性颅内非生殖细胞瘤中，有弱阳性染色表明PLAP仅存在于1例胚胎癌的少数细胞中，以及1例畸胎瘤的部分腺上皮细胞中存在；这种染色仅限于细胞质。其余6例松果体非​​生殖细胞肿瘤均未出现PLAP阳性反应。2．对 85 例（29 例颅内肿瘤和 56 例睾丸肿瘤）生殖细胞肿瘤进行了免疫组织化学研究。 52 例中可见生殖细胞瘤（精原细胞瘤）成分。其中 48 个病例 (92.3%) 含有许多 PLAP 阳性肿瘤细胞。 20例可见畸胎瘤成分。 20例中，5例（25.0%）发现有部分PLAP阳性腺上皮细胞。 14例可见胚胎癌成分。其中 4 例 (28.6%) 含有一些 PLAP 阳性肿瘤细胞。可见卵黄囊瘤成分12例。只有 1 例 (8.3%) 的多个肿瘤细胞显示 PLAP 阳性反应。 4例可见绒毛膜癌成分。 4例中，3例（75.0%）有少量合体滋养细胞肿瘤细胞呈PLAP反应阳性。 3．建立了一种用于评估 PLAP 的灵敏酶联免疫吸附测定（ELISA），并用于检查各种脑肿瘤患者的 PLAP 测量。测量了 47 名对照成年男性个体的血清和 20 名非孕期蛛网膜下腔出血患者脑脊液中的 PLAP 水平。上述样品血清和脑脊液中的上限分别为0.29和0.11 IU/L。 68 例各种脑肿瘤（预期生殖细胞瘤）（2 例畸胎瘤、1 例伴未成熟畸胎瘤的卵黄囊肿瘤、1 例睾丸转移性绒毛膜癌、14 例胶质母细胞瘤、18 例星形细胞瘤、19 例脑膜瘤和 13 例神经鞘瘤）血清中的所有 PLAP 水平均为 0.29 IU/L 或更低。 6 名生殖细胞瘤患者中有 5 名血清中的 PLAP 水平升高（0.52 至 3.78 IU/L），5 名患者中的 4 名脑脊液中 LPAP 水平也升高（0.83 至 9.83 IU/L）。 2例生殖细胞瘤瘤内囊液中PLAP水平很高（603和863 IU/L）。4．这些研究强烈表明，PLAP 不仅在组织病理学上而且在临床上都是颅内原发性生殖细胞瘤的有用肿瘤标志物。较少的

项目成果

JUN SHINODA et al.: Jounal of Neurosurgery. 63. 733-739 (1985)

JUN SHINODA 等人：神经外科杂志。

Jun Shinoda et al: "Immunohistochemicl study of primary intracranial germinomas and testicular seminomas.-Special reference to placental alkaline phosphatase-" Shinkei Kenkyu No Shinpo. 31. 127-140 (1987)

Jun Shinoda 等人：“原发性颅内生殖细胞瘤和睾丸精原细胞瘤的免疫组织化学研究。-特别参考胎盘碱性磷酸酶-” Shinkei Kenkyu No Shinpo。

篠田淳 他: 第4回日本脳腫瘍病理研究会講演集. 4. 111-118 (1987)

Jun Shinoda 等：第四届日本脑肿瘤病理学研究组论文集。4. 111-118 (1987)。

Jun Shinoda,et al.: J Neurosurg.68. 710-720 (1988)

Jun Shinoda 等人：J Neurosurg.68。

篠田淳: 第4回日本脳腫瘍病理研究会講演集. 4. 111-118 (1987)

Jun Shinoda：第四届日本脑肿瘤病理学研究组论文集。4. 111-118 (1987)。