Reconstitution of synthesized quinones to photosynthetic reaction center I

合成醌重构光合反应中心I

• 批准号: 01490028
• 负责人: ITOH Shigeru
• 金额: $ 4.22万
• 依托单位: Okazaki National Research Institutes
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01490028/
• 关键词: photosynthesis; photosystem; electron transfer; quinone; vitamin K1; herbicide; plant; energy transduction; 反応中心; 光合成反応中心; ビタミンKー1; 葉緑体; クロロフィル; 人工光合成

We have developed a method to extract and reconstitute phylloquinone which tightly binds to the plant photosynthetic photosystem I reaction center complex. The quinone, which has been known to function as vitamin K1 when fed by animal, is shown to function as the secondary electron acceptor in the photosystem I photochemistry in plant. This has made clear that all the reaction center complexes of photosynthetic organisms, from photosynthetic purple and green bacteria to higher green plants, use quinone as the secondary electron acceptor. This gives a new basis for the study of evolution of photosynthetic system as discussed in this study.After extracting phylloquinone from photosystem I reaction center, we introduced various quinone and analog compounds. They almost fully substituted for phylloquinone. Relation between the physicochemical properties and structure of these compounds and their electron transfer ability at the phylloquinone binding site in the reaction center was studied. It isoindicated that the photosynthetic reaction center proteins give unique new reaction environments for the introduced compounds. This system can also be used to test the reaction of artificial compounds in proteinaceous environments and enables the reactions impossible in solution.Structure and function of iron-sulfur clusters which function as the tertiary electron acceptors in photosystem I reaction center were also studied by cryogenic EPR.

我们已经开发了一种提取和重建phylloquinone的方法，该方法与植物光合光系统I反应中心建筑群紧密结合。奎因酮被动物喂养时已被称为维生素K1，在植物中光系统I光化学中充当二级电子受体。这清楚地表明，从光合紫色和绿色细菌到较高的绿色植物的光合生物的所有反应中心络合物，都使用喹酮作为二级电子受体。如本研究中所讨论的那样，这为光合系统的演变提供了新的基础。从光系统I反应中心提取phylloquinone后，我们引入了各种喹酮和模拟化合物。它们几乎完全代替phylloquinone。研究了这些化合物的物理化学特性与结构之间的关系及其在反应中心的phylloquinone结合位点上的电子转移能力。同样指示的是，光合反应中心蛋白为引入化合物提供了独特的新反应环境。该系统还可以用于测试蛋白质环境中人造化合物的反应，并使溶液中不可能做出反应。铁硫簇的结构和功能，这些簇充当光系统I反应中心中第三级电子受体的作用。

项目成果

Ohoka,H.,Itoh,S.,Saeki,K.,Takahashi,Y.and Matsubara,H.: "F_A/F_b protein fron the spinach photosystem I complex:Isolation in a native state and some properties of the iron-sulfur clusters." Plant & Cell Physiology.32. 11-17 (1991)

Ohoka，H.，Itoh，S.，Saeki，K.，Takahashi，Y. 和 Matsubara，H.：“菠菜光系统 I 复合体的 F_A/F_b 蛋白：天然状态下的分离和铁硫的一些特性

Hoshina,S.,Sakurai,R.,Kunishima,N.,Wada,K.and Itoh,S.: "Selective destruction of ironーsulfur centers by heat/ethylene glycol treatment and isolation of photosystem I core complex." Biochimica et Biophysica Acata. 1015. 61-68 (1990)

Hoshina, S.、Sakurai, R.、Kunishima, N.、Wada, K. 和 Itoh, S.：“通过热/乙二醇处理和光系统 I 核心复合物的分离选择性破坏铁硫中心。”生物物理学阿卡塔。1015。61-68（1990）

Takahashi,Y.,Satoh,Ki.Itoh,S.: "Silicomolybdate substitute for the function of a primary electron acceptor and stabilizes charge separatation in the photosystem II reaction center complex." FEBS Letters.255. 1333-1338 (1989)

Takahashi,Y.,Satoh,Ki.Itoh,S.：“硅钼酸盐替代主要电子受体的功能并稳定光系统 II 反应中心复合物中的电荷分离。”

S. Itoh and M. Iwaki: "Full replacement of the function of the secondary electron acceptor phylloquinone (=vitamin K_1) by non-quinone-carbonyl compounds in green plant photosystem I photosynthetic reaction centers." Biochemistry. 30. 5340-5346 (1991)

S. Itoh 和 M. Iwaki：“绿色植物光系统 I 光合反应中心中的二次电子受体叶绿醌（=维生素 K_1）的功能被非醌羰基化合物完全替代。”

Itoh,S.,Iwaki,M.: "New herbicide-binding site in the photosynthetic electron-transport chain.Competitive herbicide binding at the photosystem I phylloquinone-(vitamin K1)-binding site" FEBS Lett.250. 441-447 (1989)

Itoh,S.,Iwaki,M.：“光合电子传输链中的新除草剂结合位点。光系统 I 叶绿醌-（维生素 K1）-结合位点上的竞争性除草剂结合”FEBS Lett.250。