Optimization mechanism of electron transfer proteins

电子传递蛋白的优化机制

• 批准号: 08458214
• 负责人: ITOH Shigeru
• 金额: $ 1.34万
• 依托单位: Okazaki National Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08458214/
• 关键词: protein; electron transfer; photosynthesis; chlorophyll; Quinone; photochemical reaction; photosynthetic bacteria; cyanobacteria; 反応中心; 蛋白質; 進化

Optimization mechanisms of intra-protein electron transfer were studied in four systems below. (1) plant photosystems I and II reaction centers. (2) reaction center complexes of green sulfur bacteria. (3) A new type of photosynthetic purple bacteria that uses Zn-containing bacteriochlorophylls. (4) a new type of cyanobacteria that uses chlorophyll d which absorbs far-red light, In all the photosynthetic organisms thus far known, chlorophylls are known to be essential. We, however, showed (1) proteins function to optimize the reaction environment of chlorophylls through quinone-exchange studies in the plant photosystem I and H reaction centers, (2) Zn- bacteriochlorophyll can replace the function of Mg-bacteriochlorophyll in vivo, and (3) chlorophyll d also supports the oxygenic photosynthesis as efficient as that by chlorophyll a. These results indicate that natural photosynthetic systems have more varieties than ever estimated and that the photosynthesis with new types of chlorophylls were enabled by a minor tuning of the protein structure around chlorophylls or quinones inside the reaction center protein.

在下面的四个系统中研究了蛋白质内电子转移的优化机制。 （1）植物光系统I和II反应中心。 （2）绿硫细菌的反应中心复合物。 （3）一种使用含有Zn的细菌氯叶树的新型光合紫色细菌。 （4）一种使用叶绿素D的新型蓝细菌，它吸收了远红光，在迄今已知的所有光合生物中，叶绿素是必不可少的。 We, however, showed (1) proteins function to optimize the reaction environment of chlorophylls through quinone-exchange studies in the plant photosystem I and H reaction centers, (2) Zn- bacteriochlorophyll can replace the function of Mg-bacteriochlorophyll in vivo, and (3) chlorophyll d also supports the oxygenic photosynthesis as efficient as that by chlorophyll a.这些结果表明，天然光合系统的品种比以往任何时候都要多，并且通过对叶绿素或反应中心蛋白内叶绿素或奎因酮的蛋白质结构进行了较小的调整，具有新型叶绿素的光合作用是可以实现的。

项目成果

Saeki, K.: "Site-specific mutagenesis of Phodobacter capsulatus ferredoxin I,FdxN, that functions in nitrogen fixation-role of extra residues." J.Biol.Chem.271. 31399-31406 (1996)

Saeki, K.：“荚膜光杆菌铁氧还蛋白 I,FdxN 的位点特异性诱变，其在额外残基的固氮作用中发挥作用。”

Baba,K.et al.: "Photoinhibition of photosystem I electron transfer activity in isolated photosystem I preparations with different chlorophyll contents." Photosyntesis Research. 347. 121-130 (1996)

Baba,K.等人：“具有不同叶绿素含量的分离光系统 I 制剂中光系统 I 电子转移活性的光抑制。”

Iwaki, M.: "DELTAG_O dependence of the electron transfer rate in the photosynthetic reaction center of plant photosystem I : Natural optimization of reaction between chlorophyll a (AO) and quinone." J.Phys.Chem.100. 10802-10809 (1996)

Iwaki, M.：“植物光系统 I 光合反应中心电子转移速率的 DELTAG_O 依赖性：叶绿素 a (AO) 和醌之间反应的自然优化。”

Itoh,S.: "Dibromothymoquinone (DBMIB) replaces the function of QA at 77K in the isolated Photosystem II Reaction Center (D1-D2-cytochrome b559) complex" Plant and Cell Physiology. 37. 833-839 (1996)

Itoh,S.：“二溴百里醌 (DBMIB) 在分离的光系统 II 反应中心 (D1-D2-细胞色素 b559) 复合物中取代了 77K 的 QA 功能”《植物和细胞生理学》。

Dzuba,S.A.et al.: "Electron spin echo of spin-polarized radical pairs in the intact and quinone-reconstituted plant photosystem I reaction centers." Chemical Physics Letters. 264. 238-244 (1997)

Dzuba,S.A.等人：“完整和醌重建的植物光系统 I 反应中心中自旋极化自由基对的电子自旋回波。”